ID A0A1B6ZCR1_9SPHN Unreviewed; 932 AA.
AC A0A1B6ZCR1;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=A8B75_09520 {ECO:0000313|EMBL:OAO04260.1};
OS Sphingomonadales bacterium EhC05.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales.
OX NCBI_TaxID=1849171 {ECO:0000313|EMBL:OAO04260.1, ECO:0000313|Proteomes:UP000078455};
RN [1] {ECO:0000313|Proteomes:UP000078455}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EhC05 {ECO:0000313|Proteomes:UP000078455};
RA Rosana A., Orata F., Boucher Y., Case R.;
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAO04260.1}.
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DR EMBL; LXYP01000006; OAO04260.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1B6ZCR1; -.
DR STRING; 1849171.A8B75_09520; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000078455; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022839};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000078455};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 10..273
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 330..520
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 689..892
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
FT COILED 551..578
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 932 AA; 102057 MW; EB6F50F522ABCCE2 CRC64;
MTDISTDTQN HLYLVDGSAY IFRAYHRLPP LTNIHGEPVG AVYGYTTMLW KLADELNKAE
GPTHMAVVLD KSGTSFRNDL YDQYKANRPE APEDLRPQFP MIRDATRAFS LPLIEEENVE
ADDMIASYSK AAVAQGWKVT IVSSDKDLMQ LIEPSDDSLV DMLDTMKNVR MNRATVEEKF
GVGPEKLGDV LGLMGDSSDN IPGVPGIGPK TASKLINEFG DIESVLAAAP DMKKSKMKEN
LIEFAEQARL SRVLVTLKED CPLPDTLDSF LIQDIPENPL REFLSHHGFH SLLKKLGGNG
MATPQDPDVG ENAEAPAAAP HAPVIDRSKY ECVQDMAALE TWIARATAKG VCAVDLETDS
LESVTARVVG VCLATGPNEA CYVPLGHGSG DMFGETPQQI GLEEALAALK PLLEDDAVLK
VGQNLKYDMG VLAQHDMKIT PMDDTLVMSF NLDAGLHGHG MDELSKLHLG HECISFKSLT
GTGKKAIGFA EVPLEKATEY GAEDADVTLR LWEILRLRMA PERATRVYQM VDRPLVPVIA
KMEATGVLVD REELSRLSKT FAEEMESLEK QIHDLAGEPF SIGSPKQLGE ILFGKLGLKG
GKKGKSGQYS TDVTVLERLA GDGEPIAQKV VSWRQLAKLK STYTDALQEQ INARTGRVHT
SYSLSGAQTG RLSSTEPNLQ NIPIRTAIGR QIRQAFVAAP GNVILAADYS QIELRLAAHI
ADVETLKQAF AAGEDIHNRT ARELFGSEDR DARARAKTIN FAILYGISRW GLAKRLEVDP
DEAQDMINVY FERFPGINQY ISTTLQAARE NGYTETLFGR KTWFERLKSQ NQAERQGSER
AAINAPIQGT SADIIKRVMA RMLPALDQAG LHDVQMLMQV HDELVFELPE GDVEAASAVI
RSVMSGAAEP AVKLSVPLDV DIGTGKSWDE AH
//
