ID A0A1B7P2S2_9EURO Unreviewed; 611 AA.
AC A0A1B7P2S2;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=tyrosinase {ECO:0000256|ARBA:ARBA00011906};
DE EC=1.14.18.1 {ECO:0000256|ARBA:ARBA00011906};
GN ORFNames=ACJ72_02361 {ECO:0000313|EMBL:OAX83288.1};
OS Emergomyces africanus.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Ajellomycetaceae; Emergomyces.
OX NCBI_TaxID=1955775 {ECO:0000313|EMBL:OAX83288.1, ECO:0000313|Proteomes:UP000091918};
RN [1] {ECO:0000313|EMBL:OAX83288.1, ECO:0000313|Proteomes:UP000091918}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 136260 {ECO:0000313|EMBL:OAX83288.1,
RC ECO:0000313|Proteomes:UP000091918};
RA Cuomo C.A., Schwartz I.S., Kenyon C., de Hoog G.S., Govender N.P.,
RA Botha A., Moreno L., de Vries M., Munoz J.F., Stielow J.B.;
RT "Emmonsia species relationships and genome sequence.";
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504,
CC ChEBI:CHEBI:57924; EC=1.14.18.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000426};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924,
CC ChEBI:CHEBI:58315; EC=1.14.18.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001038};
CC -!- COFACTOR:
CC Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC Evidence={ECO:0000256|ARBA:ARBA00001973};
CC -!- SIMILARITY: Belongs to the tyrosinase family.
CC {ECO:0000256|ARBA:ARBA00009928}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAX83288.1}.
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DR EMBL; LGUA01000193; OAX83288.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1B7P2S2; -.
DR STRING; 1658172.A0A1B7P2S2; -.
DR OrthoDB; 1908494at2759; -.
DR Proteomes; UP000091918; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004503; F:tyrosinase activity; IEA:UniProtKB-EC.
DR Gene3D; 2.60.310.20; -; 1.
DR Gene3D; 1.10.1280.10; Di-copper center containing domain from catechol oxidase; 2.
DR InterPro; IPR008922; Di-copper_centre_dom_sf.
DR InterPro; IPR041640; Tyrosinase_C.
DR InterPro; IPR002227; Tyrosinase_Cu-bd.
DR PANTHER; PTHR11474:SF76; TYROSINASE; 1.
DR PANTHER; PTHR11474; TYROSINASE FAMILY MEMBER; 1.
DR Pfam; PF18132; Tyosinase_C; 1.
DR Pfam; PF00264; Tyrosinase; 2.
DR PRINTS; PR00092; TYROSINASE.
DR SUPFAM; SSF48056; Di-copper centre-containing domain; 1.
DR PROSITE; PS00497; TYROSINASE_1; 1.
DR PROSITE; PS00498; TYROSINASE_2; 1.
PE 3: Inferred from homology;
KW Melanin biosynthesis {ECO:0000256|ARBA:ARBA00023101};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000091918};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..611
FT /note="tyrosinase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5008598378"
FT DOMAIN 103..120
FT /note="Tyrosinase copper-binding"
FT /evidence="ECO:0000259|PROSITE:PS00497"
FT DOMAIN 261..272
FT /note="Tyrosinase copper-binding"
FT /evidence="ECO:0000259|PROSITE:PS00498"
SQ SEQUENCE 611 AA; 69474 MW; BE29BA4CDD60C6B8 CRC64;
MGPFRWKVLF LLNVLLGVAS AVTISGASGN VNPNTGQRPA RKDINKMQWS GPEWDIYIQA
LKAFQEEDRN DILSFHKVAG IHGYPYVSWD GVEGLGNAGY CSHGSTLFPL WHRPYLAMYE
ERISYHAKLI ADSYPPFIRR MYQQAAANLR IPYWDWANDP ELPSFPNDPL LKYRYTVRNP
NEKGESQIDA IQKTLNANGA LIRVNTYQLL ASETNYTVFS TDSLRDHRGG YNNLENIHGL
IHVSVGGNAG HMTYTPWSAY DPIFWLHHTN VDRVVALWQA LHPESYVEPL VNSGGDFMKK
PGTMEDDETP FAPFRDPDNE FYNAGTARTL STFGYTYPEI QDWNGVTPEQ LARSVRMAVN
QLYNRPGDNN KNRKRHTALT ARAAHHHGMI KNKRMPNLAE DTLDDLKNLN FKIKEFFEDL
GQFSLLNFIK LGINNLEKQW VINIRANKFA LPHAYNIHFF LSEPPEESCD WAHAPNLIGT
FASFASSMGS PKNTTRDMFG QIPLSHVLAV VWNSQMILDL KDTTVLPLLE KHLEWRVQDL
TGKVVDAGEL IGSSTGHSRG LEITIAERDV EPLAADDKER DHFPILGDWN VFQDITQKEK
KKKGCKVKYK V
//
