ID A0A1B7UMN9_9GAMM Unreviewed; 783 AA.
AC A0A1B7UMN9;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE SubName: Full=Penicillin amidase {ECO:0000313|EMBL:OBP15751.1};
GN ORFNames=A5320_10700 {ECO:0000313|EMBL:OBP15751.1};
OS Rheinheimera sp. SA_1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales; Chromatiaceae;
OC Rheinheimera.
OX NCBI_TaxID=1827365 {ECO:0000313|EMBL:OBP15751.1, ECO:0000313|Proteomes:UP000092258};
RN [1] {ECO:0000313|EMBL:OBP15751.1, ECO:0000313|Proteomes:UP000092258}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SA_1 {ECO:0000313|EMBL:OBP15751.1,
RC ECO:0000313|Proteomes:UP000092258};
RA Lavstsen T., Jespersen J.S.;
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SUBUNIT: Heterodimer of an alpha subunit and a beta subunit processed
CC from the same precursor. {ECO:0000256|ARBA:ARBA00038735}.
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBP15751.1}.
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DR EMBL; LXWK01000015; OBP15751.1; -; Genomic_DNA.
DR RefSeq; WP_068064665.1; NZ_LXWK01000015.1.
DR AlphaFoldDB; A0A1B7UMN9; -.
DR STRING; 1827365.A5320_10700; -.
DR OrthoDB; 9760084at2; -.
DR Proteomes; UP000092258; Unassembled WGS sequence.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000092258};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT ACT_SITE 254
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 186
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 330
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 333
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 783 AA; 87182 MW; 0E288B01A002A74E CRC64;
MKWLKPMFYV VLTLVAIVAI TLYSALHSSL PLYEGATSAE VTAKVQLSRD AQGYLTVRAQ
NRLDAAYGLG FAHAQDRYFQ MDLLRRNSAG ELSELFGERA LPMDISRRMH RFRDRAEAIL
TTLPAPQKLL LQHYTQGVNE GLTQLPMRPF EYWLLQQKPA AWQDTDSLLV AYSMYLDLQS
AAGADELAQG VLKQAIPDDW YQFLNQHSSD WQAAIDGSKV TAIPLPESDY PRVLSNQKVA
CQDCRLRDSR DIGSNNFSVS GKLTSHGAAL LADDMHLGIR VPGTWFKAQL IWGEGEAQHS
VAGVSLPGSP SIVAGSNGHI AWGFTNSTAD WHDVIKLQSD ENPQRYITPA GAKEYSYNNE
VIKVKGLADV VMLVKETEWG PVMPAPFGQF ALRWVAYDPQ ALNLELQQLE QVRTVDDALK
IASKVGMPAQ NLLVADKQGN HAWTLIGPIP KRTLQDMDTP QDWSTGHNFW DGYLSDTDYP
TVKNPDTQRL WTANSRVVGG DALKLLGDGG YDLGARGMQI RDGLLAVDQH SEQSLHQIQL
DHRALFLKRW QQLLLEVLTD DFVARHQLGQ YRSYVELDSA AASPASVGYS LVRAFRDQSL
QQVFAPIASL MEQQKLKLTD LKLVLETPGW ALIQAKRPDT VPAPFISWQQ LLEQAVLKSR
DALLAKTDGD LANARWGLFN QAKIEHPLSS AIPWFGQFLN MSASEMAGDR HMPRVQQPIH
GQSQRMVVAP GQEAKGILTI PAGQSGHPLS PFYRADHAFW LNEAELGFLP GEQKYLLELQ
PRG
//
