ID A0A1B7UMS4_9GAMM Unreviewed; 531 AA.
AC A0A1B7UMS4;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=Isocitrate lyase {ECO:0000256|ARBA:ARBA00017446};
DE AltName: Full=Isocitrase {ECO:0000256|ARBA:ARBA00031022};
DE AltName: Full=Isocitratase {ECO:0000256|ARBA:ARBA00031921};
GN ORFNames=A5320_10745 {ECO:0000313|EMBL:OBP15759.1};
OS Rheinheimera sp. SA_1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales; Chromatiaceae;
OC Rheinheimera.
OX NCBI_TaxID=1827365 {ECO:0000313|EMBL:OBP15759.1, ECO:0000313|Proteomes:UP000092258};
RN [1] {ECO:0000313|EMBL:OBP15759.1, ECO:0000313|Proteomes:UP000092258}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SA_1 {ECO:0000313|EMBL:OBP15759.1,
RC ECO:0000313|Proteomes:UP000092258};
RA Lavstsen T., Jespersen J.S.;
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate = glyoxylate + succinate;
CC Xref=Rhea:RHEA:13245, ChEBI:CHEBI:15562, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:36655; EC=4.1.3.1;
CC Evidence={ECO:0000256|ARBA:ARBA00023531};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR001362-3};
CC Note=Can also use Mn(2+) ion. {ECO:0000256|PIRSR:PIRSR001362-3};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBP15759.1}.
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DR EMBL; LXWK01000015; OBP15759.1; -; Genomic_DNA.
DR RefSeq; WP_068064689.1; NZ_LXWK01000015.1.
DR AlphaFoldDB; A0A1B7UMS4; -.
DR STRING; 1827365.A5320_10745; -.
DR OrthoDB; 8629576at2; -.
DR Proteomes; UP000092258; Unassembled WGS sequence.
DR GO; GO:0004451; F:isocitrate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR CDD; cd00377; ICL_PEPM; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR InterPro; IPR039556; ICL/PEPM.
DR InterPro; IPR006254; Isocitrate_lyase.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR PANTHER; PTHR21631:SF3; BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN; 1.
DR PANTHER; PTHR21631; ISOCITRATE LYASE/MALATE SYNTHASE; 1.
DR Pfam; PF00463; ICL; 3.
DR PIRSF; PIRSF001362; Isocit_lyase; 3.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE 4: Predicted;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:OBP15759.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR001362-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001362-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000092258}.
FT ACT_SITE 222
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-1"
FT BINDING 184
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-3"
SQ SEQUENCE 531 AA; 58389 MW; AF6055ADD6F482B2 CRC64;
MSAYQQNIKT LEQTLQAQGS AWNAISAEYA ARMQLQNRFK TGLQIAQYTA DIMRADMAAY
DADSSQYTQS LGCWHGFIGQ QKLIAIKKHF GSTKRRYLYL SGWMIAALRS EFGPQPDQSM
HEKTAVPALI EELYTFLRQA DARELGGLFN ELDAARAADD QTKVAAVQGK IDSFETHVVP
IIADIDAGFG NAEATYLLAK KMIEAGACAI QIENQVSDEK QCGHQDGKVT VPHADFLAKI
NAVRYAFLEL GIENGIIVAR TDSLGAGLTK QIAVTNEPGD LGDLYNAFLD GDYIESAADI
QNGDVVVKAN GKLLKPKRLQ SGLFQFKQGS GVDRVVLDCI TSLQHGADLL WIETEKPHVG
QIAEMVDRIR AVVPNAKLVY NNSPSFNWTL NFRQQVFDAW QADGKDVAAY DRAKLMSVDY
DSTDLALEAD ERIRTFQADG AKRAGIFHHL ITLPTYHTAA LSTDNLAKEY FGAAGMLGYV
KGVQRQEIRQ GIACVKHQNM SGSDIGDDHK EYFAGEAALK AGGKHNTMNQ F
//
