ID A0A1B8ABF3_FUSPO Unreviewed; 2280 AA.
AC A0A1B8ABF3;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:OBS17794.1};
GN ORFNames=FPOA_09526 {ECO:0000313|EMBL:OBS17794.1};
OS Fusarium poae.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=36050 {ECO:0000313|EMBL:OBS17794.1, ECO:0000313|Proteomes:UP000091967};
RN [1] {ECO:0000313|EMBL:OBS17794.1, ECO:0000313|Proteomes:UP000091967}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2516 {ECO:0000313|EMBL:OBS17794.1,
RC ECO:0000313|Proteomes:UP000091967};
RA Vanheule A., Audenaert K., Warris S., Van De Geest H., Schijlen E.,
RA Hofte M., De Saeger S., Haesaert G., Waalwijk C., Van Der Lee T.;
RT "Living apart together: crosstalk between the core and supernumerary
RT genomes in a fungal plant pathogen.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] =
CC ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate;
CC Xref=Rhea:RHEA:13501, Rhea:RHEA-COMP:10505, Rhea:RHEA-COMP:10506,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:83144, ChEBI:CHEBI:83145,
CC ChEBI:CHEBI:456216; EC=6.3.4.14;
CC Evidence={ECO:0000256|ARBA:ARBA00000861};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + ATP + hydrogencarbonate = ADP + H(+) + malonyl-
CC CoA + phosphate; Xref=Rhea:RHEA:11308, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57384, ChEBI:CHEBI:456216; EC=6.4.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001455};
CC -!- COFACTOR:
CC Name=biotin; Xref=ChEBI:CHEBI:57586;
CC Evidence={ECO:0000256|ARBA:ARBA00001953};
CC -!- PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from
CC acetyl-CoA: step 1/1. {ECO:0000256|ARBA:ARBA00004956}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBS17794.1}.
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DR EMBL; LYXU01000004; OBS17794.1; -; Genomic_DNA.
DR STRING; 36050.A0A1B8ABF3; -.
DR OMA; PTPKGHC; -.
DR UniPathway; UPA00655; UER00711.
DR Proteomes; UP000091967; Unassembled WGS sequence.
DR GO; GO:0003989; F:acetyl-CoA carboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004075; F:biotin carboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:2001295; P:malonyl-CoA biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd06850; biotinyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.40.50.20; -; 1.
DR Gene3D; 3.30.1490.20; ATP-grasp fold, A domain; 1.
DR Gene3D; 3.30.470.20; ATP-grasp fold, B domain; 1.
DR Gene3D; 2.40.460.10; Biotin dependent carboxylase carboxyltransferase; 1.
DR Gene3D; 3.90.1770.10; PreATP-grasp domain; 1.
DR InterPro; IPR049076; ACCA.
DR InterPro; IPR049074; ACCA_BT.
DR InterPro; IPR034733; AcCoA_carboxyl_beta.
DR InterPro; IPR013537; AcCoA_COase_cen.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR005481; BC-like_N.
DR InterPro; IPR001882; Biotin_BS.
DR InterPro; IPR011764; Biotin_carboxylation_dom.
DR InterPro; IPR005482; Biotin_COase_C.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR005479; CbamoylP_synth_lsu-like_ATP-bd.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR011763; COA_CT_C.
DR InterPro; IPR011762; COA_CT_N.
DR InterPro; IPR016185; PreATP-grasp_dom_sf.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR45728:SF3; ACETYL-COA CARBOXYLASE; 1.
DR PANTHER; PTHR45728; ACETYL-COA CARBOXYLASE, ISOFORM A; 1.
DR Pfam; PF08326; ACC_central; 1.
DR Pfam; PF21385; ACCA_BT; 1.
DR Pfam; PF02785; Biotin_carb_C; 1.
DR Pfam; PF00289; Biotin_carb_N; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF01039; Carboxyl_trans; 1.
DR Pfam; PF02786; CPSase_L_D2; 1.
DR SMART; SM00878; Biotin_carb_C; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 2.
DR SUPFAM; SSF56059; Glutathione synthetase ATP-binding domain-like; 1.
DR SUPFAM; SSF52440; PreATP-grasp domain; 1.
DR SUPFAM; SSF51246; Rudiment single hybrid motif; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS50979; BC; 1.
DR PROSITE; PS00188; BIOTIN; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR PROSITE; PS50989; COA_CT_CTER; 1.
DR PROSITE; PS50980; COA_CT_NTER; 1.
DR PROSITE; PS00866; CPSASE_1; 1.
DR PROSITE; PS00867; CPSASE_2; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00409}; Biotin {ECO:0000256|ARBA:ARBA00023267};
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00409}; Reference proteome {ECO:0000313|Proteomes:UP000091967}.
FT DOMAIN 62..570
FT /note="Biotin carboxylation"
FT /evidence="ECO:0000259|PROSITE:PS50979"
FT DOMAIN 220..411
FT /note="ATP-grasp"
FT /evidence="ECO:0000259|PROSITE:PS50975"
FT DOMAIN 697..771
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 1520..1858
FT /note="CoA carboxyltransferase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50980"
FT DOMAIN 1862..2177
FT /note="CoA carboxyltransferase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50989"
FT REGION 1219..1242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2280 AA; 255394 MW; 117EC956C6D7AB56 CRC64;
MTEISAGAQE GANGRSVPYV NGKQSYAEKF NIADHFIGGN RLANAPPSKV KDFVGQNDGH
TVITNVLIAN NGIAAVKEIR SVRKWAYETF GDERAIHFTV MATPEDLQAN AEYIRMADHY
VEVPGGTNNH NYANVELIVD IAERMNVHAV WAGWGHASEN PKLPESLAAS PNKIVFIGPP
GSAMRSLGDK ISSTIVAQHA AVPCIPWSGT GVDQVAVDDK GIVTVADDIY AKGCVTSWEE
GLEKAKEIGF PVMIKASEGG GGKGIRKATE EEGFEALYKA AASEIPGSPI FIMKLAGNAR
HLEVQLLADQ YGNNISLFGR DCSVQRRHQK IIEEAPVTIA KADTFKAMED AAVRLGKLVG
YVSAGTVEYL YSHADDKFYF LELNPRLQVE HPTTEMVSGV NLPAAQLQIA MGIPLHRIRD
IRLLYGVDPK TSSDIDFEFK NEETASSQRR PQPKGHTTAC RITSEDPGEG FKPSNGVMHE
LNFRSSSNVW GYFSVSSQGG IHSFSDSQFG HIFAYGENRS ASRKHMVMAL KELSIRGDFR
TTIEYLIKLL ETEAFEDNTI STGWLDELIS KRLTAERPET MLAVTCGAVT KAHIASEACM
TEYRAGLEKG QVPSKDVLKT VFTIDFIYEG FRYKFTATRA SVDSYHLFIN GSKCHVGVRS
LSDGGILVLL DGRSHSVYWK EEVGATRLSV DSKTCLLEQE NDPTQLRSPS PGKLVKYSVE
NGAHVRAGQA FAEVEVMKMY MPLLAQEDGV VQLIKQPGAT LEAGDILGIL ALDDPSRVKQ
AQAFVDKLPA YGEPVVVGAK PAQRFSLLYN TLQNILLGYD NSVIMASTLK ELIEVLRDTE
LPYSEWNAQF SALHSRMPQK LDAQFTQIIE RGKSRNSDFP AKALSKAFHK FIEDNVDAND
AGLLKTTLAP LTEIIDMYID GQKNRELTVI KSILEQYYEV EALFMNQPQE DAVILQLRDQ
NKDDIMKVVH TVLSHSRVSS KSSLILAILE EYRPNKPKAG NVAKNLRETL RLLTELQSRH
TSKVSLKARE IMIQCALPSL EERTSQMEHI LRSSVVESRY GETGWDHREP SLDIIKEVVD
SKYTVFDVLT MFFAHDDPWV SLASLEVYVR RAYRAYLLKQ IEYHQDENDN PQFVSWDFQL
RKLGQSEFGL PLQSAAPSTP ATPSGGEFNF KRIHSISDMS YLSSKWEDEP TRKGVIVPCK
YIDEAEDLIG KALEALAHEQ KQKKKNTPGL IPDLSGKRKP APLKQSEEEL SAVINVAIRD
VESRDDREAL EDILPIVEQY KDELLARGVR RLTFICGHSD GSYPGYYTFR GPEYKEDDSI
RHSEPALAFQ LELARLSKFN IKPVFTENKS IHVYEGIGKK HDTDKRYFTR AVIRPGRLRD
EIPTAEYLIS EADRVVNDIF DALEIIGNNN SDLNHVFINF SPVFQLQPKE VEESLQGFLD
RFGIRAWRLR IAQVEIRIIC TDPQTGEPYP LRVVITNTSG YVVDVDMYAE RKSEKGEWVF
HSIGGTHEKG PMHLMPVSTP YAIKNWLQPK RHDAHGMGTQ YVYDFPELFR QAIQNTWNKA
VKAQPSLASQ QPKTGDCISF TELVLDDKDN LDEVNREPGT NTCGMVGWIF RARTPEYPNG
RRFIVIANDI TYKIGSFGPK EDEFFHKCTE LARKLGIPRI YLSANSGARL GLADELMGHY
KVAWNNPEKQ DAGFKYLYLD DAAKTRFEKD VITEEVTEDG EKRHKIVTII GKEEGLGVEC
LRGSGLIAGA TSRAYNDIFT VTLVTCRSVG IGAYLVRLGQ RAVQIEGQPI ILTGAAALNN
LLGREVYTSN LQLGGTQIMH RNGVSHMTAN DDFAGVSKIV EWMSFVPEKR NSPVPVSPSV
DDWNRDVTYY PPQKQPYDVR WLIGGREGEN GFESGLFDKD SFVEALGGWA KTVVVGRARL
GGIPMGVIGV EVRSVENITP ADPANPDSIE QVSNEAGGVW YPNSAFKTAQ AINDFNNGEQ
LPLMILANWR GFSGGQRDMY NEVLKYGSFI VDALVKYEQP IFIYIPPFGE LRGGSWVVVD
PTINPTAMEM YADTEARGGV LEPEGMIGIK YRKQKQIQTI IRMDPTYAGL KKQLEDSSLS
TEQTDEIKKK MAAREKELLP VYSQIALQFA DLHDRAGRMK AKGVIRDVLE WSESRRFFYW
RLRRRLNEEY ILRRMTSTII STSHQAAAKD KETRDKYLHL LRSWSAIVDW ETNDQAVTEW
YETERKTISE KVEALKSEVL AAEVANVVRG HAKAGWTGVR EVMRVMPVEE REQLLKYLQQ
//
