ID A0A1B8EY75_9PEZI Unreviewed; 779 AA.
AC A0A1B8EY75;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 22-FEB-2023, entry version 22.
DE RecName: Full=Diphthine--ammonia ligase {ECO:0000256|ARBA:ARBA00018426};
DE EC=6.3.1.14 {ECO:0000256|ARBA:ARBA00012089};
DE AltName: Full=Diphthamide synthase {ECO:0000256|ARBA:ARBA00029814};
DE AltName: Full=Diphthamide synthetase {ECO:0000256|ARBA:ARBA00031552};
GN ORFNames=VF21_05010 {ECO:0000313|EMBL:OBT76217.1};
OS Pseudogymnoascus sp. 05NY08.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1622149 {ECO:0000313|EMBL:OBT76217.1, ECO:0000313|Proteomes:UP000092096};
RN [1] {ECO:0000313|EMBL:OBT76217.1, ECO:0000313|Proteomes:UP000092096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=05NY08 {ECO:0000313|EMBL:OBT76217.1,
RC ECO:0000313|Proteomes:UP000092096};
RA Palmer J.M., Drees K.P., Foster J.T., Lindner D.L.;
RT "Comparative genomics of Pseudogymnoascus destructans, the fungus causing
RT white-nose syndrome of bats.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000092096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=05NY08 {ECO:0000313|Proteomes:UP000092096};
RX PubMed=29295979; DOI=10.1038/s41467-017-02441-z;
RA Palmer J.M., Drees K.P., Foster J.T., Lindner D.L.;
RT "Extreme sensitivity to ultraviolet light in the fungal pathogen causing
RT white-nose syndrome of bats.";
RL Nat. Commun. 9:35-35(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + diphthine-[translation elongation factor 2] + NH4(+) =
CC AMP + diphosphate + diphthamide-[translation elongation factor 2] +
CC H(+); Xref=Rhea:RHEA:19753, Rhea:RHEA-COMP:10172, Rhea:RHEA-
CC COMP:10174, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:82696,
CC ChEBI:CHEBI:456215; EC=6.3.1.14;
CC Evidence={ECO:0000256|ARBA:ARBA00001559};
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DR EMBL; KV458819; OBT76217.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1B8EY75; -.
DR STRING; 1622149.A0A1B8EY75; -.
DR OrthoDB; 103959at2759; -.
DR Proteomes; UP000092096; Unassembled WGS sequence.
DR GO; GO:0017178; F:diphthine-ammonia ligase activity; IEA:UniProtKB-EC.
DR CDD; cd06155; eu_AANH_C_1; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.90.1490.10; putative n-type atp pyrophosphatase, domain 2; 1.
DR Gene3D; 3.30.1330.40; RutC-like; 2.
DR InterPro; IPR002761; Diphthami_syn_dom.
DR InterPro; IPR030662; DPH6/MJ0570.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR035959; RutC-like_sf.
DR InterPro; IPR006175; YjgF/YER057c/UK114.
DR PANTHER; PTHR12196:SF2; DIPHTHINE--AMMONIA LIGASE; 1.
DR PANTHER; PTHR12196; DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN; 1.
DR Pfam; PF01902; Diphthami_syn_2; 1.
DR Pfam; PF01042; Ribonuc_L-PSP; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF55298; YjgF-like; 2.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000092096}.
FT DOMAIN 146..270
FT /note="Diphthamide synthase"
FT /evidence="ECO:0000259|Pfam:PF01902"
FT REGION 48..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 120..139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 48..70
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 779 AA; 80980 MW; 9258A0018C485F04 CRC64;
MAPQPKPLNV IALISGGKDS IYSLLHVLSL GHRVLALGNL YPQAAAHVTS TPSTTSTSTT
TSSTTAATGI TADDDAPDPD SHMYQTAGHA LIPAIARALG LPLFRAPIVG GAVVGGSTYA
PLPGGSGTGK EKGEGGGDET ESLLPLLQAI KKAHPSADAV SAGAILSTYQ RTRIESVAAR
MGLVPLAFLW QYPSIFKEDG EAGLLAHMEE VGMEARIVKV ASGGLGEGDL WVDVVSQSGR
ERVGRKVGRF GGTGGVIGEG GEYETVVVSG GKGWWSGKVG EGERRVVSGE GGTAWVEFEK
GEVVVGEGEG GGEGGEVKVR KPALLEKSFE ETLKAVLAAM EPAAAEDQGA GIEDTAAPFS
ASATWKTTAH PDSSTWTIVP SSISSNPPTA AEAMASISTQ LSALLTTHNL SIKDIVSTTL
LLTSMSTFAS TNSVYASLFS APNPPSRACV SVGSLLPDSA PVVMHISLSS APARRALHVQ
SRSYWAPANI GPYSQAQCAP LLPGKGVEGV SIAGQIPLVP ATMEVVTAAD QALAQEKEGG
EVGLQIVLAL QHLWRVGLAM DVSFFASGTA VMARCGLEVA RGRARLAGVA WENRHRRVVE
GEEDGEEEDV DLWELTNRRG EEMMGGEKQE KMLPEWENVE GEEEGLGVSP FWAAEVEELP
RSVDVEWLAG AGVVGESVKV RSVVSGGGSG VHECVVGGKV VITTMFVGYE EAKGKGLVRV
LLGGVPGKEE LGGAEMVYLD AGIEELWGEV GGNVVPCRSL WDKEGTRLGA VVVFKSEVL
//
