ID A0A1B8G5L4_9PEZI Unreviewed; 597 AA.
AC A0A1B8G5L4;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=tRNA(Ile)-lysidine synthetase {ECO:0000256|ARBA:ARBA00013267};
DE EC=6.3.4.19 {ECO:0000256|ARBA:ARBA00013267};
GN ORFNames=VE02_00022 {ECO:0000313|EMBL:OBT91135.1};
OS Pseudogymnoascus sp. 03VT05.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1622148 {ECO:0000313|EMBL:OBT91135.1};
RN [1] {ECO:0000313|EMBL:OBT91135.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=03VT05 {ECO:0000313|EMBL:OBT91135.1};
RA Palmer J.M., Drees K.P., Foster J.T., Lindner D.L.;
RT "Comparative genomics of Pseudogymnoascus destructans, the fungus causing
RT white-nose syndrome of bats.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +
CC diphosphate + H(+) + lysidine(34) in tRNA(Ile2);
CC Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665,
CC ChEBI:CHEBI:456215; EC=6.3.4.19;
CC Evidence={ECO:0000256|ARBA:ARBA00000047};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; KV459351; OBT91135.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1B8G5L4; -.
DR Proteomes; UP000091872; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd01992; PP-ATPase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01161; tRNA_Ile_lys_synt; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR011063; TilS/TtcA_N.
DR InterPro; IPR012094; tRNA_Ile_lys_synt.
DR InterPro; IPR012795; tRNA_Ile_lys_synt_N.
DR NCBIfam; TIGR02432; lysidine_TilS_N; 1.
DR PANTHER; PTHR43033; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR PANTHER; PTHR43033:SF1; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR Pfam; PF01171; ATP_bind_3; 2.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598}.
FT DOMAIN 52..186
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
FT DOMAIN 236..289
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
SQ SEQUENCE 597 AA; 67134 MW; B50133291D01F62A CRC64;
MVGILHRTGW SGRGAAAISL DRFIASLRRL NHKGLNQLTE THGKPSKPLT IGLAISGGVD
SMALASLCSG IQNKDRFRFR AFVVDHKVRA GSGDEAHAVA KILEEKDIPT DVLKIEWPEG
LSPKEASNFE SLARTYRFQA LGKACRDHDI QSLFLAHHRD DQAETILMRM INGHRKDGLC
GMRLSGDIPE CRGIYGVHQS GVVDESATAS QIKFGRGVDL NKSRSLDSLD DNTSSLGMRI
ESGGIKVYRP LLCFTKQELR DTCEANDMSW FEDKTNADPT LTMRNAIRHI YANNKLPVAL
QKPSILALAK RLQENRDGDL ERRNKSFTQK VQILKFEPSV PYCSVYFEPL DIGHNTKSST
EISSTEFEDA SWWLRAVIQL ISPVENIALG TLSTAVQRIY PELDSQAGHD SAPRKAFTVA
GVMFEPAPSD MHDSLLSSHT GYSEGSTLAP QQIANRHWIV RPQPYKRGKE PRLEFKPLQG
KDEGAYWRDN DSWKLFDGRF WIRILNLTSS NISLRPFQPE DVNKLKGTKF MEQLEQREKF
LLPLKASKFR YVLPAIVAEV NGMEQVVGLP TLGIVAQDME KLVKYEVRYR DLDSWPN
//
