ID A0A1B9DWS7_9FLAO Unreviewed; 923 AA.
AC A0A1B9DWS7;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE SubName: Full=Aconitate hydratase {ECO:0000313|EMBL:OCB74139.1};
GN Name=acnB {ECO:0000313|EMBL:GEL09558.1};
GN ORFNames=FBGL_03040 {ECO:0000313|EMBL:OCB74139.1}, FGL01_02970
GN {ECO:0000313|EMBL:GEL09558.1};
OS Flavobacterium glycines.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=551990 {ECO:0000313|EMBL:OCB74139.1, ECO:0000313|Proteomes:UP000093226};
RN [1] {ECO:0000313|Proteomes:UP000093226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 105008 {ECO:0000313|Proteomes:UP000093226};
RA Shin S.-K., Yi H.;
RT "Draft genome sequence of Paenibacillus glacialis DSM 22343.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:OCB74139.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NBRC 105008 {ECO:0000313|EMBL:OCB74139.1};
RA Ploux O.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:GEL09558.1, ECO:0000313|Proteomes:UP000321579}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 105008 {ECO:0000313|EMBL:GEL09558.1,
RC ECO:0000313|Proteomes:UP000321579};
RA Hosoyama A., Uohara A., Ohji S., Ichikawa N.;
RT "Whole genome shotgun sequence of Flavobacterium glycines NBRC 105008.";
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis-
CC aconitate + H2O; Xref=Rhea:RHEA:17941, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:57429, ChEBI:CHEBI:57872; EC=4.2.1.99;
CC Evidence={ECO:0000256|ARBA:ARBA00000118};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OCB74139.1}.
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DR EMBL; BJVF01000001; GEL09558.1; -; Genomic_DNA.
DR EMBL; LVEO01000004; OCB74139.1; -; Genomic_DNA.
DR RefSeq; WP_066324962.1; NZ_LVEO01000004.1.
DR AlphaFoldDB; A0A1B9DWS7; -.
DR STRING; 551990.SAMN05192550_1441; -.
DR OrthoDB; 9758061at2; -.
DR Proteomes; UP000093226; Unassembled WGS sequence.
DR Proteomes; UP000321579; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 3.40.1060.10; Aconitase, Domain 2; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR Gene3D; 1.25.40.310; Aconitate B, HEAT-like domain; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR015933; Aconitase_B_HEAT-like_dom.
DR InterPro; IPR036288; Aconitase_B_HEAT-like_dom_sf.
DR InterPro; IPR015929; Aconitase_B_swivel.
DR InterPro; IPR015932; Aconitase_dom2.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF4; ACONITATE HYDRATASE B; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF06434; Aconitase_2_N; 1.
DR Pfam; PF11791; Aconitase_B_N; 1.
DR SUPFAM; SSF74778; Aconitase B, N-terminal domain; 1.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 6..162
FT /note="Aconitase B HEAT-like"
FT /evidence="ECO:0000259|Pfam:PF11791"
FT DOMAIN 176..405
FT /note="Aconitase B swivel"
FT /evidence="ECO:0000259|Pfam:PF06434"
FT DOMAIN 410..894
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
SQ SEQUENCE 923 AA; 100218 MW; F244B5AF6A98871B CRC64;
MNIYNDYIKE IEERKAQGLH PKPIDGAELL SEIITQIKDL DNANREDSLQ FFIYNALPGT
TPAAGVKAKF LKEIILGESI VAEITPDFAF ELLSHMKGGP SIEVLLDLAL GNDAAIAQQA
ADVLKTQVFL YEADTDRLKS AYNSGNAIAK EILESYAKAE FFTKLPEVAE EIKVVTFIAG
EGDISTDLLS PGNQAHSRSD RELHGKCMIT PEAQEEIKAL QAQHPDKSVM LIAEKGTMGV
GSSRMSGVNN VALWTGKQAS PYVPFVNFAP IVGGTNGISP IFLTTVDVTG GIGIDLKNWV
KKTDADGNLV RNESGEPVLE QAYSVATGTV LTINTKTKKL YNGDQELIDI SRSFTPQKME
FIKAGGSYAI VFGKKLQTFA AKTLGVEAPV VFAPSKEISH EGQGLTAVEK IFNRNAVGTT
PGKVLHAGSD VRVTVNIVGS QDTTGLMTAQ ELESMAATVI SPVVDGAYQS GCHTASVWDK
KAQANIPKLM KFMNDFGLIT ARDPKGIYHS MTDVIHKVLN DITIDEWAII IGGDSHTRMS
KGVAFGADSG TVALALATGE ASMPIPESVK VTFKGDMKSY MDFRDVVHAT QQQMLKQFGG
ENVFQGRIIE VHIGTLTADQ AFTFTDWTAE MKAKASICIS EDETLIESLE IAKGRIQIMI
DKGMDNAKQV LKGLINKANK RIAEIKSGEK PALTPDANAK YYAEVVVDLD VIAEPMIADP
DVNNADVSKR YTHDTIRPLS YYGGEKKVDL GFIGSCMVHK GDMKILAQML KNVEAQKGKV
EFNAPLVVAP PTYNIVDELK AEGDWEVLQR YSGFEFDDNA PKGAARTEYE NMLYLERPGC
NLCMGNQEKA SKGDTVMATS TRLFQGRVVE DSAEKKGESL LSSTPVVVLS TILGRTPTIE
EYTKAVEGIN LTKFAPSHKL LVK
//
