ID A0A1C0A6T6_9FIRM Unreviewed; 667 AA.
AC A0A1C0A6T6;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=U472_08030 {ECO:0000313|EMBL:OCL25967.1};
OS Orenia metallireducens.
OC Bacteria; Bacillota; Clostridia; Halanaerobiales; Halobacteroidaceae;
OC Orenia.
OX NCBI_TaxID=1413210 {ECO:0000313|EMBL:OCL25967.1, ECO:0000313|Proteomes:UP000093514};
RN [1] {ECO:0000313|EMBL:OCL25967.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Z6 {ECO:0000313|EMBL:OCL25967.1};
RA Dong Y., Sanford R.A., Fouke B.W.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:OCL25967.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Z6 {ECO:0000313|EMBL:OCL25967.1};
RA Maxim B.I., Kenneth K., Flynn T.M., Oloughlin E.J., Locke R.A., Weber J.R.,
RA Egan S.M., Mackie R.I., Cann I.K.;
RT "Orenia metallireducens sp. nov. strain Z6, a Novel Metal-reducing
RT Firmicute from the Deep Subsurface.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OCL25967.1}.
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DR EMBL; LWDV01000009; OCL25967.1; -; Genomic_DNA.
DR RefSeq; WP_068717309.1; NZ_LWDV01000009.1.
DR AlphaFoldDB; A0A1C0A6T6; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000093514; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000093514}.
FT DOMAIN 5..289
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 290..579
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 667 AA; 77247 MW; 29351C3243300FAE CRC64;
MNLSLALNAK QLEAVKTTEG PVLVLAGAGS GKTRALTYRI AYLINKDVDP YNILTLTFTN
KAAEDMKRKV EELIGDISKR MWMGTFHSVC VRIISQNIKA LGYDSGFVIY DQNESKDLVS
DIILTMNLDK EKYDAKIVLN MINKAKMNMW SKRELINNYL EIGGGSEEFY QNIGDVYLKY
NEVLKDNNAF DFNDLIGKTI ELFESNEDIL KRYQDRFKYI LIDEYQDTSP FQYKLASMLA
GKHQNIFVVG DDYQSIYKFR GADMENILNF NEDYPECKTI KLEQNYRSKK NIIEASNALI
SNNYRQFDKK AWTEKDEGEP IIICEALNEY GEADYVAEEI DNLIRFTNYS YNDVAILYRS
NHQSRVLEEA FIRRRIPYHI VGGLGFYDRK EIKDIISYLK VAVNPQDTIA LKRIVNAPKR
GIGAKTLDKL INHAQKYVPE FNLFSFSQED NSLGLFDVMK DPTGVSGIGK KTAERIKQFR
RDLEDVITVK ESRITLPEKV DKIMKVSGYQ AMLELDGSET ALNRLDNLAE FLALTENYYN
KNQDRDLEDF VRDLKLLSDQ DDMNNSNQIK MMTVHASKGL EFPVIFIVGM EEDTFPHYRS
VKTGMLEEIE EERRLCYVAM TRAADRLFMT YARERQRYGE TRSMTPSRFL DEIPTELILE
GNHYRNL
//
