ID A0A1C0ZWA8_9BACL Unreviewed; 731 AA.
AC A0A1C0ZWA8;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=A8709_31655 {ECO:0000313|EMBL:OCT12386.1};
OS Paenibacillus pectinilyticus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=512399 {ECO:0000313|EMBL:OCT12386.1, ECO:0000313|Proteomes:UP000093309};
RN [1] {ECO:0000313|Proteomes:UP000093309}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KCTC13222 {ECO:0000313|Proteomes:UP000093309};
RA Zhang J., Zhang X.;
RT "Paenibacillus oryzae. sp. nov., isolated from the rice root.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC {ECO:0000256|ARBA:ARBA00006202}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OCT12386.1}.
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DR EMBL; LYPC01000027; OCT12386.1; -; Genomic_DNA.
DR RefSeq; WP_065856635.1; NZ_LYPC01000027.1.
DR AlphaFoldDB; A0A1C0ZWA8; -.
DR STRING; 512399.A8709_31655; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000093309; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000093309}.
FT DOMAIN 30..286
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 650..726
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 479
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 549
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT BINDING 200
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 367..368
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 444
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 477..481
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 527
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
FT BINDING 549
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-2"
SQ SEQUENCE 731 AA; 82698 MW; F99F85DE3D539265 CRC64;
MSISFEPTQG IFHLQNKHMS YVLQIVHSTY LAHAYWGRPI RSGQLGSIIQ YTERASFSPN
PDTKNRVFSL DTLPQEFPAY GTSDFREPAY QVALPNGSTI TELVYDKHRI VQGKPGLEGL
PSTYVEADHE AETLEIELVD KLAGLRAILS YTIFANHAAI TRSVRFINEG SADLKLLRAL
SMSLDYAHAD WDLLQLSGSW ARERHIERRA LAPGKLTVES RRGSSSHSHN PFVALLSKEA
NEDHGDVYGF NLVYSGNFAA HAEVDPFATA RVYMGINPFD FSWLLEPGQQ FQTPEVVMVY
SSNGLGEMSR AYHRLYRTRL CRGEYRDQVR PVLVNNWEAT YFNFNADKIE SIARAGKELG
IELFVLDDGW FGKRNDDTTS LGDWVVDKVK LPGGIEDLVE RVNNLGLEFG LWFEPEMVSP
ESDLYRNHPD WCLHVPDRRR TEGRGQLILD FSREDVREGV MGMLRNILSS APITYVKWDM
NRNMTEIGSA SLPSERQRET AHRYILGLYD VLEKITSEYP HILFESCSGG GGRFDPGMLH
YMPQTWTSDN TDAVCRLKIQ YGTSIVYPVS SMGSHVSAVP NHQVHRTTSL ETRGHVAMSG
NFGYELDLTK FTDEEKEIVK AQVADYKKIR PLVQFGDMYR LLSPFEGNDT AWMIVSEDKT
QAMVVFIRVL AQPYAPLKTL RLKGLNPDFD YQIEGSEGTF AGDHLLYAGL SVAGLHSDFE
SKIWLLEAKP Q
//
