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Database: UniProt
Entry: A0A1C1CGV4_9EURO
LinkDB: A0A1C1CGV4_9EURO
Original site: A0A1C1CGV4_9EURO 
ID   A0A1C1CGV4_9EURO        Unreviewed;       808 AA.
AC   A0A1C1CGV4;
DT   02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   02-NOV-2016, sequence version 1.
DT   03-JUL-2019, entry version 19.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   Name=ure1 {ECO:0000313|EMBL:OCT47739.1};
GN   ORFNames=CLCR_03286 {ECO:0000313|EMBL:OCT47739.1};
OS   Cladophialophora carrionii.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae;
OC   Cladophialophora.
OX   NCBI_TaxID=86049 {ECO:0000313|EMBL:OCT47739.1, ECO:0000313|Proteomes:UP000094526};
RN   [1] {ECO:0000313|EMBL:OCT47739.1, ECO:0000313|Proteomes:UP000094526}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSF {ECO:0000313|EMBL:OCT47739.1,
RC   ECO:0000313|Proteomes:UP000094526};
RA   Noorani M.;
RL   Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222,
CC         ECO:0000256|PIRSR:PIRSR001222-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:OCT47739.1}.
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DR   EMBL; LGRB01000013; OCT47739.1; -; Genomic_DNA.
DR   STRING; 86049.XP_008727165.1; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000094526; Unassembled WGS sequence.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PIRSF; PIRSF001222; Urease; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 1.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000094526};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222,
KW   ECO:0000256|PIRSR:PIRSR001222-51};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-
KW   51}; Reference proteome {ECO:0000313|Proteomes:UP000094526}.
FT   DOMAIN      371    807       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    562    562       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       376    376       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       378    378       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       459    459       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       459    459       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       488    488       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       514    514       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       602    602       Nickel 1. {ECO:0000256|PIRSR:PIRSR001222-
FT                                51}.
FT   BINDING     461    461       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     459    459       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   808 AA;  86754 MW;  867A9B6DF7A79E4F CRC64;
     MQLVPKEALI ANNLQELIRD GNHSVADLMS IGKTMLGRRH VLPSVVSTLT ELQVEGTFKS
     GTYLVTVHHP VSSDDGDLEK ALYGSFLPVP SKDAFPLAEA SEYESAKMPG AVVPVKEGKI
     TLNEGRKRIQ LKVTSKGDRP IQIGSHYHFI ETNPQLEFDR ARAYGFRLDI PAGTSVRFEP
     GDAKTVTLVE IAGDKVIHGG NNIASGAVDV SRTDEIIRKL QEAGFAHTPE PAGDLAHIDV
     AAMSRADYAG MFGPTTGDLV RLGSTDLWIK VEKDMTVYGD ECKFGGGKTI RDGMGQASDR
     KDEDVLDTVI TNALIVDWSG IYKADIGIKN GVIAGIGKAG NPDVMEGVDP NLVVGNGTDV
     IAGEHSIVTA GGFDSHIHLI CPQQAYESVA AGITTYLGGG TGPSTGTNAT TCTPGKWHMK
     QMLQALDSFP INYGVTGKGN DSSPVALREQ CEAGACGLKL HEDWGTTPAA IDTCLSVCDE
     YDVQCLIHTD TLNESGFVES SIAAFKGRTI HTYHTEGAGG GHAPDIISVV EHANVLPSST
     NPTRPYTRNT LDEHLDMLMV CHHLSKNIPE DVAFAESRIR AETIAAEDVL HDLGAISMMS
     SDSQAMGRCG EVILRTWNTA QKNKQQRGTL KEDEGTDADN FRVKRYVSKY TINPAITQGM
     SHILGSVEVG KLADLVLWKP SHFGTKPSLV LKGGMIAYAQ MGDPNASIPT VQPVIMRPMF
     GAYVPSTSIT FVSGASIASG QVASYNLAKR VEAVKHCRTV GKKDMKFNDS MPKMKVDPES
     YRVEADGMHC TAEPAEVVPL GQTYYVRG
//
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