ID A0A1C4F1M4_9ENTR Unreviewed; 533 AA.
AC A0A1C4F1M4;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=Malate synthase {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
GN ORFNames=GA0061070_102956 {ECO:0000313|EMBL:SCC49583.1};
OS Kosakonia oryziphila.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Kosakonia.
OX NCBI_TaxID=1005667 {ECO:0000313|EMBL:SCC49583.1, ECO:0000313|Proteomes:UP000198515};
RN [1] {ECO:0000313|Proteomes:UP000198515}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=REICA_142 {ECO:0000313|Proteomes:UP000198515};
RA Varghese N., Submissions Spin;
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699,
CC ECO:0000256|RuleBase:RU000555};
CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC isocitrate: step 2/2. {ECO:0000256|RuleBase:RU000555}.
CC -!- SIMILARITY: Belongs to the malate synthase family.
CC {ECO:0000256|ARBA:ARBA00006394, ECO:0000256|RuleBase:RU000555}.
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DR EMBL; FMBC01000029; SCC49583.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1C4F1M4; -.
DR OrthoDB; 9768429at2; -.
DR UniPathway; UPA00703; UER00720.
DR Proteomes; UP000198515; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR019830; Malate_synthase_CS.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR048356; MS_N.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR NCBIfam; TIGR01344; malate_syn_A; 1.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF20656; MS_N; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
DR PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435,
KW ECO:0000256|RuleBase:RU000555};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000555};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU000555}.
FT DOMAIN 10..71
FT /note="Malate synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20656"
FT DOMAIN 162..407
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 412..531
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 166
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 447
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 533 AA; 60500 MW; 39826169ADC3B4E3 CRC64;
MTQQATTIDE LAFTQPYGEQ ERQILTPEAV EFLSELVTRF TPKRNQLLAA RQRQQQQIDA
GTLPGFISET ASIRESDWTI RGVPEDLLDR RVEITGPVER KMVINALNAN VKVFMADFED
SLAPNWDKVI DGQINLRDAV NGTISYTNEA GKIYQLKPNP AVLVCRVRGL HLPEKHVTWR
GEAIPGSLFD FALYFFHNHK NLLAKGSGPY FYLPKTQSWQ EAAWWSEVFS YTEDRFNLPR
GTIKATLLIE TLPAVFQMDE ILHALRDHIV GLNCGRWDYI FSYIKTLKNY PDRVLPDRQV
VTMDKPFLSA YSRLLIKTCH RRGAFAMGGM AAFIPSKDAE RNNWVLNKVK ADKELEARNG
HDGTWIAHPG LGDTVMDVFN AALGDNKNQL FISRKDDAPI TAEQLLAPCE GERTEEGMRA
NIRVAVQYIE AWISGNGCVP IYGLMEDAAT AEISRTSIWQ WIHHEKTLSN GKPVTKTLFR
QMLAEEMLVI QDELGEQRFS SGRFDDAARL MEQITTSDEL IDFLTLPGYR LLA
//
