ID A0A1C5BX94_9ACTN Unreviewed; 1179 AA.
AC A0A1C5BX94;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=GA0115254_108627 {ECO:0000313|EMBL:SCF62938.1};
OS Streptomyces sp. Ncost-T10-10d.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1839774 {ECO:0000313|EMBL:SCF62938.1, ECO:0000313|Proteomes:UP000198750};
RN [1] {ECO:0000313|Proteomes:UP000198750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ncost-T10-10d {ECO:0000313|Proteomes:UP000198750};
RA Varghese N., Submissions Spin;
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; FMDF01000071; SCF62938.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1C5BX94; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000198750; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000198750};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1179 AA; 130843 MW; 7EFFC0FD49D5E1FE CRC64;
MTKPPFTHLH VHTQYSLLDG AARLKDMFAA CNEMGMSHIA MTDHGNLHGA YDFFHSAQKA
GVTPIIGIEA YVAPESRKHK RKIQWGQPHQ KRDDVSGSGG YTHKTIWASN ATGLHNLFRL
SSDAYAEGWL QKWPRMDKET IAQWSEGLIA STGCPSGEVQ TRLRLGQFDE AVQAASDYKD
IFGEGKYFLE LMDHGIEIER RVRDGLLEIG KKLNIPPLVT NDSHYTYAHE ATAHDALLCI
QTGKNLSDPD RFRFDGTGYY LKTTDEMYAV DSSDAWQEGC ANTLLVAQQI DTGGMFEKRD
LMPKFEIPDG FTEITWFQEE VRRGMERRFP GGVPDDRQKQ VEYEMDIIIQ MGFPGYFLVV
ADFIMWAKNN GIAVGPGRGS AAGSIVAYAM GITDLDPIEH GLIFERFLNP ERVSMPDVDI
DFDERRRVEV IRYVTEKYGA DKVAMIGTYG KIKAKNAIKD SARVLGYPYA MGDRLTKAMP
ADVLGKGIDL NGITDPKHPR YSEAGEIRGM YENEPDVKKV IDTAKGVEGL VRQMGVHAAG
VIMSSEPIVD HAPIWVRHTD GVTITQWDYP QCESLGLLKM DFLGLRNLTI MDDAVKMVES
NKGVKLDLLG LPLDDPKTYE MLCRGDTLGV FQFDGGPMRS LLRQMQPDNF EDISAVSALY
RPGPMGMNSH TNYAERKNGR QEITPIHPEL EEPLKEVLGL TYGLIVYQEQ VQKAAQIVAG
YSLGEADILR RVMGKKKPEE LAKNFVLFEA GAKEKGFSDA AIKALWDVLV PFAGYAFNKA
HSSAYGLVTY WTAYLKANYP AEYMAALLTS VKDDKDKSAV YLNECRRMGI KVLPPNVNES
LSNFAAQGDD VILFGLTAVR NVGQNVVDSI IRCRKAKGKY SSFPDFLDKV EAVVCNKRTV
ESLIKAGAFD EMGHTRKGLV AHHEPMIDNV VQVKRKEAEG QFDLFGGMGE EESDEPGFGL
DVEFSDIEWE KSYLLAQERE MLGLYVSDHP LFGLEHVLSD KADSSISQLT GGEHADGAIV
TVGGIISGLQ RKMTKQGNAW AIATVEDLAG SIECMFFPAT YQLVSTQLVE DTVVFVKGRL
DKREDVPRLV AMEMQVPDIS SAGTNAPVVL TIPTVKITPP MVSRLGEVLS NHRGDTEVRI
KLQGPRKTTV LRLDRHRVKP DPALFGDLKV LLGPSCLAG
//
