ID A0A1C5JW52_9ACTN Unreviewed; 427 AA.
AC A0A1C5JW52;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU003370};
GN ORFNames=GA0070613_5490 {ECO:0000313|EMBL:SCG74246.1};
OS Micromonospora inositola.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=47865 {ECO:0000313|EMBL:SCG74246.1, ECO:0000313|Proteomes:UP000198221};
RN [1] {ECO:0000313|EMBL:SCG74246.1, ECO:0000313|Proteomes:UP000198221}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 43819 {ECO:0000313|EMBL:SCG74246.1,
RC ECO:0000313|Proteomes:UP000198221};
RA Kjaerup R.B., Dalgaard T.S., Juul-Madsen H.R.;
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308,
CC ECO:0000256|RuleBase:RU003370};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751,
CC ECO:0000256|RuleBase:RU003370}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU003406}.
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DR EMBL; LT607754; SCG74246.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1C5JW52; -.
DR OrthoDB; 9800864at2; -.
DR UniPathway; UPA00223; UER00717.
DR Proteomes; UP000198221; Chromosome i.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06114; EcCS_like; 1.
DR Gene3D; 2.20.28.60; -; 1.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR NCBIfam; TIGR01798; cit_synth_I; 1.
DR PANTHER; PTHR42871; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR42871:SF1; CITRATE SYNTHASE; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000198221};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU003370}.
FT ACT_SITE 305
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 362
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 427 AA; 47572 MW; E8573C45710BFE31 CRC64;
MTEVKLDHPG GQLSMPVQSA VEGPAGIGVS HLLKETGMTT YDPGFVNTAS CSSAITYIDG
DAGILRYRGY PIEQLAAKSS FLEVSYLLIY GELPTQQELT EFSERIRRHS LLHEEMRRFF
DGFPRDAHPM AVLSSAVSAI STFYQDSLDP FDSQHVEMST VRLMAKVPTI ASYAYKKSIG
QPLLYPDNSL GYVENFLRMT FGVPAEPYEV DPVMARVLDM LFILHADHEQ NCSTSTVRLV
GSSNANLFAS VSAGVNALFG PLHGGANQAV LEMLQKIHAD GDDVRSFVQK VKDKQTGVKL
MGFGHRVYKN YDPRAAIVKK AAQDVLGRMA KPDPLLDLAM ELEEIALADD FFVSRRLYPN
VDFYTGLIYK AMGFPTKMFT VLFALGRLPG WIAQWREMIN DPETKIGRPR QIYTGYPERD
YPPAAER
//
