ID A0A1C6RPS2_9ACTN Unreviewed; 1131 AA.
AC A0A1C6RPS2;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=GA0070616_1689 {ECO:0000313|EMBL:SCL19206.1};
OS Micromonospora nigra.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=145857 {ECO:0000313|EMBL:SCL19206.1, ECO:0000313|Proteomes:UP000199699};
RN [1] {ECO:0000313|EMBL:SCL19206.1, ECO:0000313|Proteomes:UP000199699}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 43818 {ECO:0000313|EMBL:SCL19206.1,
RC ECO:0000313|Proteomes:UP000199699};
RA Kjaerup R.B., Dalgaard T.S., Juul-Madsen H.R.;
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; FMHT01000003; SCL19206.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1C6RPS2; -.
DR STRING; 145857.GA0070616_1689; -.
DR OrthoDB; 4812256at2; -.
DR Proteomes; UP000199699; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000199699}.
FT DOMAIN 35..364
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 365..697
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 875..896
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 56..63
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1131 AA; 121216 MW; ECCF5B952D9EC563 CRC64;
MTQPALFGTV PPVPRRPDAG PRYTPVELAR LLRLPAPTRE QAEIIAAPVE PLLVVAGAGS
GKTETMAARV VWLVANSYVR PEQVLGLTFT RKAAGELAHR VRTRLDQLVR RLGRRGRDPL
DDPLAGEPTV STYHSYAGRI VTEHGLRAGY EPSTRLLTEA SRWQLVDLIV RNYDGDMTGV
DRMPSTVTDA VLALAGELDE HLVAPDALAA WTGRFFADVQ SRPGRVYADV RRALALQQTR
LKLLPLVRAY ARRKDDFEAM DFADQLARAA RVARDHPGVG EIERDRFRVV LLDEYQDTSH
AQVVLLNALF GGGHPVTAVG DPCQSIYGWR GASAGTLDRF PTEFARSDGG PARVLGLTTS
WRNRPEILGV ANALATPLRA AGARVPELHA ALSVADPIPH RSPRGAAAGT VHCALLTTYA
DEADWIADSV LAAWRAAGGN PGALPEHIPV ARRPTTAVLV RLRSQIPAIE SALRARGLPV
EVVGLGGLLD TPEVRDVVCT LRVLADPTDG AALLRLLTGA RWRIGPRDLV ALHRRAKTLA
AARRQLADDG APEITPDLLD EATLVEALAD LGPAQAYSAE GYARLRAYAH ELGLLRYRLD
QSLPELIADI ERTIGLDVEV AVRAGHDGAG DAGLARGHLD ALGDVAARFS GETPGATLAG
FLAYLTAAED EERGLAPGEV EVVEGAVQIL TAHAAKGLEW DVVAVAGLSR GIWPGPVRNS
DHWLGGLGVL PFPLRGDADG LPHLDLAEVG DQRGVARAVE DFTDSWRAHD EREERRLAYV
AVTRPRRLLL CSGHWWGEGV KRPRGPSVLL REVHDACLDG GTGHLVDEWA PEPTPDAVNP
TTEVVLRAEW PADPLGVRRP ALAEAAALVR RNLAAGDGPG RPVPADGPAP ANGVADDPEV
ARWEREADLL LAERSELTKL SGPVEVAVPG QLSVTQLVAL RRDPEALARA LRRPLPSEPS
PYARRGTAFH AWLEQRFGAD RLLDVDELPG AADADAAPDE ALAELQERFL ASEWADRVPV
EVEVPFATVL AGVVVRGRMD AVFARPGGRF DVVDWKTGGQ PVGRAAEAAA VQLAVYRLAW
AELAGVPVDR VGAAFHYVRD GVTVRPADLL DVEGLTALIA ELPGAGIPAR G
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