ID A0A1C6U2C2_9ACTN Unreviewed; 5074 AA.
AC A0A1C6U2C2;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE SubName: Full=Amino acid adenylation domain-containing protein/natural product biosynthesis luciferase-like monooxygenase domain-containing protein {ECO:0000313|EMBL:SCL48058.1};
GN ORFNames=GA0070617_0762 {ECO:0000313|EMBL:SCL48058.1};
OS Micromonospora yangpuensis.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=683228 {ECO:0000313|EMBL:SCL48058.1, ECO:0000313|Proteomes:UP000198937};
RN [1] {ECO:0000313|EMBL:SCL48058.1, ECO:0000313|Proteomes:UP000198937}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45577 {ECO:0000313|EMBL:SCL48058.1,
RC ECO:0000313|Proteomes:UP000198937};
RA Kjaerup R.B., Dalgaard T.S., Juul-Madsen H.R.;
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- SIMILARITY: In the C-terminal section; belongs to the NRP synthetase
CC family. {ECO:0000256|ARBA:ARBA00029443}.
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DR EMBL; FMIA01000002; SCL48058.1; -; Genomic_DNA.
DR STRING; 683228.GA0070617_0762; -.
DR OrthoDB; 5478077at2; -.
DR Proteomes; UP000198937; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0008483; F:transaminase activity; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 2.
DR Gene3D; 3.20.20.30; Luciferase-like domain; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 2.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 2.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR005814; Aminotrans_3.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR024011; Biosynth_lucif-like_mOase_dom.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 1.
DR NCBIfam; TIGR04020; seco_metab_LLM; 1.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF00202; Aminotran_3; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 1.
DR Pfam; PF00296; Bac_luciferase; 1.
DR Pfam; PF00668; Condensation; 2.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF51679; Bacterial luciferase-like; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 4.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 3: Inferred from homology;
KW Monooxygenase {ECO:0000313|EMBL:SCL48058.1};
KW Oxidoreductase {ECO:0000313|EMBL:SCL48058.1};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898};
KW Reference proteome {ECO:0000313|Proteomes:UP000198937};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1288..1363
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1381..1806
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2290..2368
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4500..4575
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 125..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1220..1241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1262..1292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2243..2287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2429..2480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2566..2628
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3734..3753
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4569..4622
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2245..2269
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2458..2478
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5074 AA; 533386 MW; 59C65DC2D215745E CRC64;
MDELTEALAR RTELIDVAVV DRTGFDGVRY RVAYVVPAPT GTPAQARRAA QAAVAATGDS
GGPVPVAVVS GIPRDADGVA DPAALRAVPV ATTAGGTLVR RPDRKPDRIH LADVLELPPR
WGSDLVAPAA GPAPAVSGDT PKDPGTRPAE SAVPPRDVPA AEPDAPSAEP AVEAVGGVPE
VGGDLPATLP EALRRAARDR PEQGLRLVEA DGTRTLRYPE LLDAALRTLT GLRAAGLTTG
DPVILHLPSL ADHFVALWAC LLGGLRPVAV AQAPGYQARN ATLDKLQHAW RSLDHPVVLT
SGTVTAQLGT YADRAGWGGL RVLDLADCRA HEPAGELPRP TPDDVAMLQL SSGSTSASKV
IPLTHRGIVR YARDACQVAR VRPDDVMLNW LPLDHVAGLV MMHLGPVVLG CDNVHVGTAL
VLTDPPLWLD LMQRYRVRHS WSPNFGFKLV TEALRAQPDR RWDLSGVRTL INAGEQCTEP
VMRGFLAATA DSGLAPDALV LAWGMAETCT AISYQTFGPD AVQHAELHAD GSRVRLAPAS
APGTTTFLSM GPPAPGTRFR VTAPDGRTAL PELRIGRLQV RSERVTPGYL NHDEANRAAF
PDGPWFDTGD LAYLHDGRLT ITGRGKEVII VNGVHYFCHE IEDLVGAVPG VAPTQVAAFG
APGPDGIERI VVLFCAQEAL DAELLTRIRQ RLAQRLQVGS AHLVCVPPEQ FEKTTSGKIQ
RTSMRNRLLA GGYEPQSRRV DLLDAAPTTV ADCVHRPVWT ARRFTRATPP AGPVLVVADT
LGLAARLGRD LPDAVLVDPT GRPYDDPDRA GRPYEDPDRW VELLRSLAAG GRTPGLVVFA
ASYLPTPDLD DTDATRRAVE VCGTGPLGLL CALAALDWTG TLLTLSRGLY RIRGDEAGCP
PAALTAALGE TLGVEQPGVR AWHLDLPGDE VAGDAATVVA ACGWAHREPL VAWRGRPLVR
AVERVEPAKG PAVLRPGSRW LVTGGLGGVG RVILGDLRAE LDLRVLVVGR SEPGPALAEL
AAAGPDRVRY ARVDVTDGTA LEAAVVEAER AWGAPLDGAL HLAGRYRTVP LSAPRAGQWR
PETDPKVAGS IEVARLLRRR PGSHLVAFSS LLSLTPVVGA GGYAAANRFL EALCAQLGDA
HCLSYGMWRG VGLSEQGGVE AATRRHMLSF SVPQGQALTR LALHQPAGHL LVGFDTNATR
VRHLVRPVWA LEGVRATASS GAAAGSGTSG AATGSGVPGA AAGSGVPTDV FGVPVPVVAD
PVGSTPDAVD DASPASTAPA PGGSATAGPD PVGVANAVRQ ALRQTVPGGV DEHTAFHDAG
LDSVRLLRLH TLLQQSLGRQ FPQTTLFTHG TPAALARHLA ERFTPSGGEL AATGSADRPG
EQRIAVIGLA ARLPGADTVE RYWQNLLAGR VDVRRFSRAE LLAAGLPPAL VDDPAFVPVS
GALADIEGFD AELFGISARE AALVDPQQRL FLQVCLEALE DGGQAGTDRR VGVYAGAGMH
LYSLRNYLLD RFAGADPADQ VAALQVTIGN QPDFLATRVA YRLGLTGPAI SVQTACSTSL
VAVHLAVRAL LAGETDLALA GAAAVHVPQA AGYRYEEGSI LSRAGACRAF DAAADGTVGG
NGVAAVLLKR LDDALADGDT VYGVIIGSAV NNDGATKTGY TAPSVTGHAE VIRAALAQAR
VDADSIGYVE THGTGTRIGD PIEVEALRTV FGGRGRPLPL GAVKANIGHL DTCAGMAGLI
KAIMVVRDGK VPPVAGLRRP NAELRLDEGP FELPTEVRDW TGPGPRRAGV SSLGVGGTNA
HVVVEEPPAP VAARRSPPAG PVVVPLSAQH PTALRQLAER FADRLASADA PRPDDVLVTL
GAGRRALRHR LVAWSDSPAG VADVLRRATS TPAGAGLVAG SVPAGGPGPV VFACSGQGSD
LTGAAAGLLA HEPAREVLEQ CAGWYQRTWG VDLLAALRGE PHEWTTATVQ PALYAVQVAF
GRLLESVGVR PDVLIGHSAG EYAALCLAGA LPVADGLHLA AVRGQLMQSR TPEGAALAVL
ADAAQLAPVL AQVPGAELTV RNGPRRYVLS GTPEVVARAG ELLRAADLDH RPLAVRRAFH
SALMEPVLAE LGAQAATLDW QPLGTSLVSS LSGVRLAPGT RLDAEHVRRQ TRQPADYHAG
VEGLVADGCT TFVEIGPDAT LTALGRQWPD TSWIPVQRGR DRAGSVLAAL ATLYARGVPV
DWAALAPTGR RIPLPTYPFQ LTRHWAAPTP PPPLTPEAPH TPEAPHTPAA PLTPEAGGTP
RMSPAVPANP AADIVLRQVR ELTARHLDED PDRIAADAAF FDLGADSLLM INLLRDLEAT
YRIRVTMREL FEDLDTPQRL SQAVLERMTP ERLAALTRDE ATPDDVIAAA ATPDGVIAAA
APSAGTVPSA GTVPSTAAVA PSVPFEPAAV AGNGNGTRPH PAGPPDQTFT GLPSVAPAGP
PAVGPPPVAG PPVAGPPAAP ATVSGAAVTP AAELIRAQLD LMGRFADLMT HQLAALSASP
EPGVPPVPAP GPAPALPAPA PATSALAAPA PVMPASVMPA PVMPAPATPS TAVPSSGPAP
RSAASAPPAG EPGAAGDPVA GNGAGPAQLG PRPLRVSGGA GSGRLDERQR AHFDDLVRRY
VERTRRSKEL AQHHRPWLAD SRAVVGFRRA TKEMLYPLTA RRARGAHLED VDGNSYVDIT
MGFGALLFGH EPEFLTSAIA THLADGLRLG PRGEEAGDAA RLLCELTGMD RAAFATTGTE
ANSAAFRLAR AYTGRDLVVT FDGAYHGHFD PVLGRGVQEG GSWRTRPVSA GVPASAVADV
RVLPYGAAQS LEVIREHADR IAAVVVEPVQ SRHPDRQPGE FLHELRRLCD TYGMVLLFDE
MLTGFRPHPQ GAQGVFGVRA DLATYGKVIG GGFPVGAVAG RADIMDWVDG GFWRYGDDSV
PPQDTTFFGG TYIQHPVSMV AARAVLTELR RRGPALQEGL NRATTRLADT VNAFCVAEDF
PVRVHHFGSL FRLASPTDLN LLFNHLLLAG VHVWEWRNFF LSDAHTDADV DTVIDAVRES
LYDLRRGGFF PGGQAPPPAP ARPAEAAPAP AAASVPAVGS ALAVQSAPAV QSAPAVGSVL
AVQAAAGKGR PAGVPDLSLY FFGDYPQQRV EDRYAAVLAA ATFADRHGLH AVWLPERHFD
SFGGIFPNPS VLAAAVAART ERVRIHAGCA VLPLHDPIRV AEEWAVVDNL SGGRVGIGCA
SGWHARDFVL APQVYGRHRE AMYEAVGQIR TLWQGGALTR TAGNGEPVEV RLFPRPVQPM
PPMFAAVVGN PDSYRRAAEA DLGIITNLMS QSVEQLGENI ARYRAARAEH GLDPAAGRVV
LLLHTYLGDD TKRVRDEAFG PFCDYLRSSL ALFGQVTNSL GVTIDLANTA EEDVAFLLRR
AYRRYTTDRA LIGSPADCAS IVARVAELGV DEIGCFVDFG LPPDRMLAGL PGIEQLGALV
GHPTGPPPAV PSSPAALSSQ AVPSSQAALS SQAVPSSPAA LVDAGSSAQV APLSVAQREV
WFVERMLPGR PTYTETKVVR LTGELDVDAL RTALESVVHR HPALRSVVRE VDGEPRQLAL
PPGRLDLPVE QADASGDEAG AVDSADAGAV AAVLRQENAH RFDLAAGPLF APRLVRLAAD
RHLLVLRMHH LVIDTVSAQL VSREIAAGYR AALERRPAQL PPAPPWPAVA AEPDEETLRG
HLAYWTGALA GLPTRPDLPT DRPRPAVPSG AGGVAAAELD ADLTRRVRQF ARAHRVTPFM
VLLAGFAVLL SDLVGRREVL VGTPVAHRPP GTEQAVGMYV NTLPLRIDVA PEAGFGELVR
TVRSVLLDAQ EHQDAPLPRI VAALGGQPEP GRHPLFDVVV EFDNGAVFEL DLPGVRAELL
DAAVDRAPFD LALFLTGRAE RVDCQLNFAR DLFDRPTVTR ILDRLAVLLR AGTDQPELPV
AELPRLTDDE RERIDRWQDG GPPAPGAGRT LLAGLSLDTS TVLDQHGRLA GPQLGRRAEA
VAAALRAAGV GPGQLVAVHL PRGADAVVAM LGVLATGAGY VPLPPDQPAA RSTAILASAR
VAAVVAHTAQ PRLPVSVPTV LVDQLAPAAP GAGDPPETVA LSLQGAATAA LSRQGAATAA
LSPEDVAYVL FTSGSTGRPK GCVIPHRAIA NTVAWYVRDL GLTAADRLSW FSSPGFDATC
LEVWPALTVG ATLCVVPEEA RFDPVRLRDW LVGTRVTVAM MPTPMGELLI DLDWPAGTAL
RHLVVGGDRL RRQARAELPF RVTNGYGPTE AAVLSTWAQL TPADGAVPPI GRPVPGTWVR
VLDGYGRPVP VGAAGELHLG GAQLGLGYLD DPAETGRRFR RHPEHGPLFA TGDVVRWRPD
GRLEFLHRND AQVQIRGVRV EPAEAEYHLR QLDGVRQAAV RAWRDERDEA YLAGYVVPAD
PEVTADRLLR ALAERLPAQL VPTVCVLLPE LPLQDSGKLD RARLPEPDRG DLVATGPRVA
PATELERRLH ELWRAELGLT DLSVESSFFQ LGGTSLTALR LLNRTATELG RTVEILDFLR
DPTVRAMARR LTPPDPAGPA GLSGPAGPAG PAGPAGLSDP AGTADPAGTA DPAVAGGGSV
GDDRAAPVGE VVRTAPASFG QARQVLLTIR SDEPQTLTVA TRFALRGRLD RAALRRALTA
LADRQAVLRT RYAIEDGVVR QEVLATRPVD LPVVEVAPDR LDDTVTGWAA QPFALEREPC
FRALLVRLTA PTDRADPGER HELVLAVHHG VIDGWGVDVL VRDLGALYTA EVTGAPADLP
VLAADFLDFS RWEQRFLAEE QTRDLVRGWA EQVREQAAPV RLPTDRPRPS TVVSAGTSVR
AEIAADLVTA ANDYAVRRKV TPFVVFAAAF AGFLHELTGQ PSITLTVPVA NRLDPRFADL
VGGLAHVPWL VVPVDGAAGF DELVDRTTAA TWQTLAMQSV PLPVLTEALG ERFAGNPPRV
YFSMFDLAPP VLRLPGLDPA PARDVLLAGA RADQLWQVTP APDGAMALVV EYATALFDAD
TVTGWIAGYQ HLLRRALAGP DATLPGTSAV AAGD
//
