ID A0A1C7MCI1_GRIFR Unreviewed; 1004 AA.
AC A0A1C7MCI1;
DT 30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=Poll {ECO:0000313|EMBL:OBZ74633.1};
GN ORFNames=A0H81_05614 {ECO:0000313|EMBL:OBZ74633.1};
OS Grifola frondosa (Maitake) (Polyporus frondosus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Grifolaceae; Grifola.
OX NCBI_TaxID=5627 {ECO:0000313|EMBL:OBZ74633.1, ECO:0000313|Proteomes:UP000092993};
RN [1] {ECO:0000313|EMBL:OBZ74633.1, ECO:0000313|Proteomes:UP000092993}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=9006-11 {ECO:0000313|EMBL:OBZ74633.1,
RC ECO:0000313|Proteomes:UP000092993};
RA Min B., Park H., Kim J.-G., Cho H., Oh Y.-L., Kong W.-S., Choi I.-G.;
RT "Whole genome sequencing of Grifola frondosa 9006-11.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-X family.
CC {ECO:0000256|ARBA:ARBA00008323}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OBZ74633.1}.
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DR EMBL; LUGG01000005; OBZ74633.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1C7MCI1; -.
DR STRING; 5627.A0A1C7MCI1; -.
DR OrthoDB; 49764at2759; -.
DR Proteomes; UP000092993; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd00141; NT_POLXc; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 1.10.150.110; DNA polymerase beta, N-terminal domain-like; 1.
DR Gene3D; 3.30.210.10; DNA polymerase, thumb domain; 1.
DR InterPro; IPR001357; BRCT_dom.
DR InterPro; IPR002054; DNA-dir_DNA_pol_X.
DR InterPro; IPR019843; DNA_pol-X_BS.
DR InterPro; IPR010996; DNA_pol_b-like_N.
DR InterPro; IPR028207; DNA_pol_B_palm_palm.
DR InterPro; IPR018944; DNA_pol_lambd_fingers_domain.
DR InterPro; IPR027421; DNA_pol_lamdba_lyase_dom_sf.
DR InterPro; IPR037160; DNA_Pol_thumb_sf.
DR InterPro; IPR022312; DNA_pol_X.
DR InterPro; IPR002008; DNA_pol_X_beta-like.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR029398; PolB_thumb.
DR PANTHER; PTHR11276:SF43; DNA POLYMERASE IV; 1.
DR PANTHER; PTHR11276; DNA POLYMERASE TYPE-X FAMILY MEMBER; 1.
DR Pfam; PF14792; DNA_pol_B_palm; 1.
DR Pfam; PF14791; DNA_pol_B_thumb; 1.
DR Pfam; PF10391; DNA_pol_lambd_f; 1.
DR Pfam; PF14716; HHH_8; 1.
DR PRINTS; PR00869; DNAPOLX.
DR PRINTS; PR00870; DNAPOLXBETA.
DR SMART; SM00483; POLXc; 1.
DR SUPFAM; SSF47802; DNA polymerase beta, N-terminal domain-like; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81585; PsbU/PolX domain-like; 1.
DR PROSITE; PS50172; BRCT; 1.
DR PROSITE; PS00522; DNA_POLYMERASE_X; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000092993};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 353..440
FT /note="BRCT"
FT /evidence="ECO:0000259|PROSITE:PS50172"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 29..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 129..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 168..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 507..596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 611..645
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 252..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 332..346
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..523
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..595
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 735
FT /note="Nucleophile; Schiff-base intermediate with DNA; for
FT 5'-dRP lyase activity"
FT /evidence="ECO:0000256|PIRSR:PIRSR622312-50"
SQ SEQUENCE 1004 AA; 110031 MW; CF1A57162A152681 CRC64;
MSEVSRHTQE SFGALDAIMN LPEETSQELA ELRARAARGE RIPHPSHPSL PLMQDFNRDS
VATAPAKESL PSPQAATDEP TSTSIRNELQ LSRTEMLIEQ HGSRLLQPGL LKSSVASGKR
SATEALLEHA ERTKKKKKRS SSSSVHDPVV RATRTKATTN MTLITEAGPS SSAHIEVPES
SSSAPGVNCK GKSTSSISKM SQAISAVSVP SGPLDVDMRI VDAIANIHRN VGPSSDKHKT
GPHADTSKPV QQGPKIRVTK SSNQVASGPK PIPQDKILKI PASESDTSSA QARIACNNNI
TTPAISEVLH ASAPSASVPE VAVKVEDKKA TASPLSKKEK GCRAGTSRGE KPTVQQYLKG
RRIFYYGNDL RYAGVETRGR MDFIVEHGGT LVPKYDPAQI THIVTEADQL LFLSDIGLQS
LDEIPDHIPT VEWSWVLSGL GRGGYKHPKE ANIKGKVECD DSGEQSEDEE EMLAPMDWEY
KHASFRDQLD ADKLPWMKPG EIMPKVKGRK KQLIDKGDAS RTEIVQDSGE LSHISPFTHD
KVPPRSATTG PPAGKQISPS SPIIPYPDDS RPGASTRNGE HSTAGKMQNT GFEPHSFSAV
DPLAEFYARA RAESDAEPCG DDESGPSIRS DTETDDEKDL PYKRRKVARG KVTRKGGFIC
DTKDGKKDGP CANQDVVDKL LELKKLHEAH ATNEDHWRVY SYNKSLKGIR DYPKRIKSFD
EARTIYGVGE KTARKIMEII DKGTLDRIRF EKTEKTQVAM FFMGIYGVGP AIAENWYNAG
CRTLEDVKAG KGGIKLTAAQ SIGLEYYSDI NTRMPRSEVE EIFNMIKPIA LEIDPDLYIE
VMGSFRRGRA DCGDIDILIT RPTADGRTHR ENYDDLELIY RGYVARALTG CEANRSVGTL
LASRGAALLY YTGDDGFNRS VRLKANRMGY SLNQRGLYGG VVRNPCNRRQ KTNAGAPDPP
PSVTISPTAE FSLSLGNIIA SETEQEILAI LNVPWQEPHE RIRN
//
