ID A0A1D2I6A8_9ACTN Unreviewed; 684 AA.
AC A0A1D2I6A8;
DT 30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2016, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=Neutral metalloproteinase {ECO:0000256|RuleBase:RU366073};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU366073};
GN ORFNames=APS67_005829 {ECO:0000313|EMBL:ODA70032.1};
OS Streptomyces sp. AVP053U2.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1737066 {ECO:0000313|EMBL:ODA70032.1, ECO:0000313|Proteomes:UP000054731};
RN [1] {ECO:0000313|EMBL:ODA70032.1, ECO:0000313|Proteomes:UP000054731}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AVP053U2 {ECO:0000313|EMBL:ODA70032.1,
RC ECO:0000313|Proteomes:UP000054731};
RA Olsen C.W., Carey S., Hinshaw L., Karasin A.I.;
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Extracellular zinc metalloprotease.
CC {ECO:0000256|RuleBase:RU366073}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU366073};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU366073}.
CC -!- SIMILARITY: Belongs to the peptidase M4 family.
CC {ECO:0000256|ARBA:ARBA00009388, ECO:0000256|RuleBase:RU366073}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ODA70032.1}.
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DR EMBL; LMTQ02000047; ODA70032.1; -; Genomic_DNA.
DR RefSeq; WP_042175924.1; NZ_LMTQ02000047.1.
DR AlphaFoldDB; A0A1D2I6A8; -.
DR OrthoDB; 291295at2; -.
DR Proteomes; UP000054731; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09597; M4_TLP; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 3.10.450.490; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR002884; P_dom.
DR InterPro; IPR023612; Peptidase_M4.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR001570; Peptidase_M4_C_domain.
DR InterPro; IPR013856; Peptidase_M4_domain.
DR PANTHER; PTHR33794; BACILLOLYSIN; 1.
DR PANTHER; PTHR33794:SF1; BACILLOLYSIN; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF01483; P_proprotein; 1.
DR Pfam; PF01447; Peptidase_M4; 1.
DR Pfam; PF02868; Peptidase_M4_C; 1.
DR PRINTS; PR00730; THERMOLYSIN.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
DR PROSITE; PS51829; P_HOMO_B; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366073};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU366073};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU366073};
KW Reference proteome {ECO:0000313|Proteomes:UP000054731};
KW Secreted {ECO:0000256|RuleBase:RU366073};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU366073};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366073}.
FT SIGNAL 1..45
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT CHAIN 46..684
FT /note="Neutral metalloproteinase"
FT /evidence="ECO:0000256|RuleBase:RU366073"
FT /id="PRO_5023144555"
FT DOMAIN 558..684
FT /note="P/Homo B"
FT /evidence="ECO:0000259|PROSITE:PS51829"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 44..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 374
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
FT ACT_SITE 461
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR623612-1"
SQ SEQUENCE 684 AA; 71421 MW; 9F0E744916278AC5 CRC64;
MRRRSSHRRT PHAGPRPTHR RTAAVALAGV AALVATAVQA GSATAAPDAT PAARPKPAAE
SVPLTPAQRA ELIREANATK ADTAEDLGLG TKEKLVVRDV LKDRDGTVHV RYERTYDGLP
VLGGDLVAQG ERPGAAESVV KATRAAVEPA TTTATVSTAE AKQQALSAAK AEDAKSPDVD
RAPRKVVWAA SGTPTVAYET VVGGLQHDGT PQELHVVTDA TTGEKLYEWQ AIQNATGHTV
YSGTVELTST QSGAAYNLTD GARGGHRTYN LNRGTSGTGT LFSGSDDVWG NGSPSNLESA
AADAHYGAAL TWDYYKNVHG RSGIRGDGVG AYSRVHYGNN YVNAFWSDSC FCMTYGDGSG
NVNPLTSIDV AAHEMTHGLT SNTAGLIYSG ESGGLNEATS DIFGATVEFH AANSSDVGDY
LIGEEIDING DGTPLRYMDR PSRDGASKDA WYSGIGSIDV HYSSGPANHF FYLLSEGSGT
KTINGVSYDS PTSDGLPVTG IGRAKAEKIW FRALTTKFTS TTNYAGARTG TLAATGELYG
TDSAEYKAVQ DAWAGINVGA RSGDGGGGGT TFENTTDVSI PDNGAAVTSS VTVTGQAGNA
PSNLRVPVDI VHTWRGDLVV DLLAPDGTAY RLKSRSSGDS ADNVNETYTV NASSETANGT
WQLRVQDQAA RDTGHINSWG LTFP
//
