ID A0A1D2V8V5_9ASCO Unreviewed; 1069 AA.
AC A0A1D2V8V5;
DT 30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Sterol 3-beta-glucosyltransferase {ECO:0000256|ARBA:ARBA00017894};
DE EC=2.4.1.173 {ECO:0000256|ARBA:ARBA00012650};
DE AltName: Full=Autophagy-related protein 26 {ECO:0000256|ARBA:ARBA00029843};
DE Flags: Fragment;
GN ORFNames=ASCRUDRAFT_17082 {ECO:0000313|EMBL:ODV58064.1};
OS Ascoidea rubescens DSM 1968.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Ascoideaceae; Ascoidea.
OX NCBI_TaxID=1344418 {ECO:0000313|EMBL:ODV58064.1, ECO:0000313|Proteomes:UP000095038};
RN [1] {ECO:0000313|Proteomes:UP000095038}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 1968 {ECO:0000313|Proteomes:UP000095038};
RG DOE Joint Genome Institute;
RA Riley R., Haridas S., Wolfe K.H., Lopes M.R., Hittinger C.T., Goker M.,
RA Salamov A., Wisecaver J., Long T.M., Aerts A.L., Barry K., Choi C.,
RA Clum A., Coughlan A.Y., Deshpande S., Douglass A.P., Hanson S.J.,
RA Klenk H.-P., Labutti K., Lapidus A., Lindquist E., Lipzen A.,
RA Meier-Kolthoff J.P., Ohm R.A., Otillar R.P., Pangilinan J., Peng Y.,
RA Rokas A., Rosa C.A., Scheuner C., Sibirny A.A., Slot J.C., Stielow J.B.,
RA Sun H., Kurtzman C.P., Blackwell M., Grigoriev I.V., Jeffries T.W.;
RT "Comparative genomics of biotechnologically important yeasts.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside +
CC H(+) + UDP; Xref=Rhea:RHEA:22724, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15889, ChEBI:CHEBI:37424, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; EC=2.4.1.173;
CC Evidence={ECO:0000256|ARBA:ARBA00035583};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22725;
CC Evidence={ECO:0000256|ARBA:ARBA00035583};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D-
CC glucoside + H(+) + UDP; Xref=Rhea:RHEA:61836, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16933, ChEBI:CHEBI:52973, ChEBI:CHEBI:58223,
CC ChEBI:CHEBI:58885; Evidence={ECO:0000256|ARBA:ARBA00035586};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:61837;
CC Evidence={ECO:0000256|ARBA:ARBA00035586};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC Membrane {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC {ECO:0000256|ARBA:ARBA00006962}.
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DR EMBL; KV454495; ODV58064.1; -; Genomic_DNA.
DR RefSeq; XP_020044371.1; XM_020189465.1.
DR AlphaFoldDB; A0A1D2V8V5; -.
DR STRING; 1344418.A0A1D2V8V5; -.
DR GeneID; 30963101; -.
DR InParanoid; A0A1D2V8V5; -.
DR OrthoDB; 76239at2759; -.
DR Proteomes; UP000095038; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0102203; F:brassicasterol glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0102205; F:cholesterol alpha-glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0051506; F:ergosterol UDP-glucosyltransferase activity; IEA:RHEA.
DR GO; GO:0102202; F:soladodine glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0030259; P:lipid glycosylation; IEA:InterPro.
DR GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR CDD; cd13215; PH-GRAM1_AGT26; 1.
DR CDD; cd13216; PH-GRAM2_AGT26; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 3.
DR InterPro; IPR048066; ATG26_PH_GRAM1.
DR InterPro; IPR048065; ATG26_PH_GRAM2.
DR InterPro; IPR010610; EryCIII-like_C.
DR InterPro; IPR004276; GlycoTrans_28_N.
DR InterPro; IPR004182; GRAM.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR002213; UDP_glucos_trans.
DR PANTHER; PTHR48050; STEROL 3-BETA-GLUCOSYLTRANSFERASE; 1.
DR PANTHER; PTHR48050:SF25; STEROL 3-BETA-GLUCOSYLTRANSFERASE; 1.
DR Pfam; PF06722; EryCIII-like_C; 1.
DR Pfam; PF03033; Glyco_transf_28; 1.
DR Pfam; PF02893; GRAM; 2.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00568; GRAM; 2.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000095038};
KW Steroid biosynthesis {ECO:0000256|ARBA:ARBA00022955};
KW Steroid metabolism {ECO:0000256|ARBA:ARBA00023221};
KW Sterol biosynthesis {ECO:0000256|ARBA:ARBA00023011};
KW Sterol metabolism {ECO:0000256|ARBA:ARBA00023166};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ODV58064.1}.
FT DOMAIN 157..188
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT REGION 920..942
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ODV58064.1"
FT NON_TER 1069
FT /evidence="ECO:0000313|EMBL:ODV58064.1"
SQ SEQUENCE 1069 AA; 121635 MW; 60F6BA22FA4DC311 CRC64;
IKEGFQNVNE KKLSKALSLS SKLASTFNID PDDEFLDDFS CWLLRDVLLQ GHLYITKSFI
LFFAYLPKLT KDSTIFDGNL STKRNYNSKL KLKLISQNPI SSNNRYWCVL KNSTFSTFNN
SSDLYFPLLT IDLRYAIRVE IIPNAPNIED DQKPCWFRIF TETKTYKFKA DNLYSARQWV
AALKKQIFTA RNKGDIVTVK LPISNLIDLE ETSIFEDSET IRLKVLENSK TFAVDDYFFM
FFHNGHNVIN CINQALSLNN LPNFELSSGS TESSPVILSN STIDDLIRSK NLSSILSSSR
QIIPVEEKIQ SLVSINRRQR ALGFNISIDI DSDNDICNRD NRNEISEILN SGYEDSDVNE
VESSNENDDL LTHKNRFLGN TGTKALQIIN PKNLVKNMFV LNTLSNVSGM WTAEPSHYEE
KNSLERGLED EYFVASEIER KNSIRRFQSH FSLPEGEKLI STYYAHFQRN IPLYGKIYLG
SNEICFRSLI PGTNTKMILP FNDIETCYKE KGFRFGIGYS GLVIVIHAHE ELFFEFSSSE
SRDDCETLLL KQIDLLKNYN YRFNNYSFII NNNSGKEEED IKLAESVNNQ AEAARIKCFE
NKLNEETGFD LPIIIEDKKF KEKLKPNKNM HFTLLTIGSR GDVQPYLALG RGLLKEGHKV
RIATHKEFEK VVLSNGIEFR EVSGDPTKLM SFMGDHGGIS VSFIKDGKAL FSNWINELLE
SSWEACQGTD VLIESPSAMA GIHIAEALCI PYFRAFTMPW TRTRAYPHAF IVPEQKMGGS
YNYLTHIIFE TVFWKGISGQ VNRWRKERLG LERTNLELMQ QNKVPFLYNI SPTVFPSPVD
FSDWVKVTGY WFLNEGTDYI PTEKLKKFIN KARRDKKKLV YIGFGSIVVN DPRELTRAIV
EAVVDSDVRC ILNKGWSGRN SGTAGDERKE EEDGEGEGEE EEALPACIYS SGSVPHDWLF
FQVDAAVHHG GSGTTGASLR AGLPTVVKPF FGDQFFYGNR VEDIGVGVSL KKLNSRTLGR
AIREVTTSKR IIEKARAIGE KIRKENGVET AIGIIYGELD YARNLILAK
//
