ID A0A1D2W4N1_9EURY Unreviewed; 158 AA.
AC A0A1D2W4N1;
DT 30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2016, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Transcription factor E {ECO:0000256|HAMAP-Rule:MF_01909};
DE Short=TFE {ECO:0000256|HAMAP-Rule:MF_01909};
DE AltName: Full=TFIIE subunit alpha homolog {ECO:0000256|HAMAP-Rule:MF_01909};
DE AltName: Full=Transcription initiation factor TFIIE {ECO:0000256|HAMAP-Rule:MF_01909};
GN Name=tfe {ECO:0000256|HAMAP-Rule:MF_01909};
GN ORFNames=A9507_15305 {ECO:0000313|EMBL:OEC84501.1};
OS Methanobacterium sp. A39.
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanobacterium.
OX NCBI_TaxID=1860100 {ECO:0000313|EMBL:OEC84501.1, ECO:0000313|Proteomes:UP000094736};
RN [1] {ECO:0000313|EMBL:OEC84501.1, ECO:0000313|Proteomes:UP000094736}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A39 {ECO:0000313|EMBL:OEC84501.1,
RC ECO:0000313|Proteomes:UP000094736};
RA Woodcroft B.J.;
RT "Validation of picogram- and femtogram-input DNA libraries for microscale
RT metagenomics.";
RL Submitted (JUN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor that plays a role in the activation of
CC archaeal genes transcribed by RNA polymerase. Facilitates transcription
CC initiation by enhancing TATA-box recognition by TATA-box-binding
CC protein (Tbp), and transcription factor B (Tfb) and RNA polymerase
CC recruitment. Not absolutely required for transcription in vitro, but
CC particularly important in cases where Tbp or Tfb function is not
CC optimal. It dynamically alters the nucleic acid-binding properties of
CC RNA polymerases by stabilizing the initiation complex and destabilizing
CC elongation complexes. Seems to translocate with the RNA polymerase
CC following initiation and acts by binding to the non template strand of
CC the transcription bubble in elongation complexes. {ECO:0000256|HAMAP-
CC Rule:MF_01909}.
CC -!- SUBUNIT: Monomer. Interaction with RNA polymerase subunits RpoF and
CC RpoE is necessary for Tfe stimulatory transcription activity. Able to
CC interact with Tbp and RNA polymerase in the absence of DNA promoter.
CC Interacts both with the preinitiation and elongation complexes.
CC {ECO:0000256|HAMAP-Rule:MF_01909}.
CC -!- DOMAIN: The winged helix domain is involved in binding to DNA in the
CC preinitiation complex. {ECO:0000256|HAMAP-Rule:MF_01909}.
CC -!- SIMILARITY: Belongs to the TFE family. {ECO:0000256|HAMAP-
CC Rule:MF_01909}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEC84501.1}.
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DR EMBL; LZPM01000055; OEC84501.1; -; Genomic_DNA.
DR RefSeq; WP_069585642.1; NZ_LZPM01000055.1.
DR AlphaFoldDB; A0A1D2W4N1; -.
DR STRING; 1860100.A9507_15305; -.
DR Proteomes; UP000094736; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation at RNA polymerase II promoter; IEA:InterPro.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR HAMAP; MF_01909; TFE_arch; 1.
DR InterPro; IPR016481; TF_E_archaea.
DR InterPro; IPR017919; TFIIE/TFIIEa_HTH.
DR InterPro; IPR002853; TFIIE_asu.
DR InterPro; IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; TIGR00373; transcription factor E; 1.
DR Pfam; PF02002; TFIIE_alpha; 1.
DR PIRSF; PIRSF006373; TF_E_archaea; 1.
DR SMART; SM00531; TFIIE; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51344; HTH_TFE_IIE; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_01909}; Reference proteome {ECO:0000313|Proteomes:UP000094736};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_01909};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_01909}.
FT DOMAIN 3..87
FT /note="HTH TFE/IIEalpha-type"
FT /evidence="ECO:0000259|PROSITE:PS51344"
SQ SEQUENCE 158 AA; 18679 MW; E06354BF2F0ACC01 CRC64;
MLNETMVQEL IHEVTEDNEH SLTIIKCLLE GKITDEEISE EIEVRLNIVR RILYKLYDAG
LASYKRSKDP ETQWYTYSWT FEKDSVIETI SKNYEEKAEE IEESLAYEED NMFFVCTNGC
RFKFEEAAEY NFICPECDET LEYQDNTSVI SGLKEQMN
//
