ID   A0A1D3L3H5_9EURY        Unreviewed;       317 AA.
AC   A0A1D3L3H5;
DT   30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   30-NOV-2016, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   SubName: Full=Malate dehydrogenase {ECO:0000313|EMBL:SCG86202.1};
DE            EC=1.1.1.37 {ECO:0000313|EMBL:SCG86202.1};
GN   Name=mdh 3 {ECO:0000313|EMBL:SCG86202.1};
GN   ORFNames=MCBB_1647 {ECO:0000313|EMBL:SCG86202.1};
OS   Methanobacterium congolense.
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanobacterium.
OX   NCBI_TaxID=118062 {ECO:0000313|EMBL:SCG86202.1, ECO:0000313|Proteomes:UP000094707};
RN   [1] {ECO:0000313|EMBL:SCG86202.1, ECO:0000313|Proteomes:UP000094707}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Buetzberg {ECO:0000313|EMBL:SCG86202.1};
RA   Seilhamer J.J.;
RL   Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily.
CC       {ECO:0000256|ARBA:ARBA00008104, ECO:0000256|RuleBase:RU003369}.
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DR   EMBL; LT607756; SCG86202.1; -; Genomic_DNA.
DR   RefSeq; WP_071907284.1; NZ_LT607756.1.
DR   AlphaFoldDB; A0A1D3L3H5; -.
DR   STRING; 118062.MCBB_1647; -.
DR   GeneID; 30412485; -.
DR   KEGG; mcub:MCBB_1647; -.
DR   PATRIC; fig|129848.4.peg.1685; -.
DR   OrthoDB; 2596at2157; -.
DR   Proteomes; UP000094707; Chromosome I.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR   Gene3D; 3.90.110.10; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR022383; Lactate/malate_DH_C.
DR   InterPro; IPR001236; Lactate/malate_DH_N.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR43128; L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+)); 1.
DR   PANTHER; PTHR43128:SF34; L-LACTATE DEHYDROGENASE; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   3: Inferred from homology;
KW   NAD {ECO:0000256|PIRSR:PIRSR000102-3};
KW   NADP {ECO:0000256|ARBA:ARBA00022857};
KW   Oxidoreductase {ECO:0000256|RuleBase:RU003369,
KW   ECO:0000313|EMBL:SCG86202.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000094707}.
FT   DOMAIN          1..144
FT                   /note="Lactate/malate dehydrogenase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00056"
FT   DOMAIN          148..310
FT                   /note="Lactate/malate dehydrogenase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF02866"
FT   ACT_SITE        177
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000102-1"
FT   BINDING         7..13
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
FT   BINDING         84
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000102-2"
FT   BINDING         90
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000102-2"
FT   BINDING         97
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
FT   BINDING         120..122
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
FT   BINDING         122
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000102-2"
FT   BINDING         153
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000102-2"
SQ   SEQUENCE   317 AA;  34455 MW;  231619B16F271412 CRC64;
     MKVSVIGASG KVGKTAAFCL AEENLVDELV LIAREKSIDK VEGEALDMYD ALAAKDVNVS
     ITPSCDLKDI RDSDVVVLSA GIPRSSGMTR MDVAVPNAKI VAEYSRAVAR YAPDSVLLVV
     TNPVDVMTYI AFKASGFDKN KVFGLGNHLD SLRLKNQIAK HFNIHVSEIH TRVVGEHGNH
     MVPLISSTSI GGILLKYFSK YDSFDIARTI EKVKSAGEYV ISKKGATEYG PAYAVSNIVN
     IILNDEKKIL TVSAYLEGEI EGVPDVCLGV PVKLGKDGIR RIIPIKMSEE EKVAFLEAAK
     FVKESTNEVL DCLDEDF
//