ID A0A1D5UJQ1_WHEAT Unreviewed; 1283 AA.
AC A0A1D5UJQ1;
DT 30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=DNA replication ATP-dependent helicase/nuclease {ECO:0000256|RuleBase:RU367041};
DE EC=3.1.-.- {ECO:0000256|RuleBase:RU367041};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU367041};
GN ORFNames=CAMPLR22A2D_LOCUS4519 {ECO:0000313|EMBL:SPT19893.1},
GN CFC21_031635 {ECO:0000313|EMBL:KAF7018337.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS2D02G451700.1};
RN [1] {ECO:0000313|EMBL:KAF7018337.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7018337.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS2D02G451700.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS2D02G451700.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EMBL:SPT19893.1, ECO:0000313|Proteomes:UP000280104}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Thind KAUR A.;
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EnsemblPlants:TraesCS2D02G451700.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [5] {ECO:0000313|EMBL:KAF7018337.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7018337.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Key enzyme involved in DNA replication and DNA repair.
CC Involved in Okazaki fragments processing by cleaving long flaps that
CC escape FEN1: flaps that are longer than 27 nucleotides are coated by
CC replication protein A complex (RPA), leading to recruit DNA2 which
CC cleaves the flap until it is too short to bind RPA and becomes a
CC substrate for FEN1. Also involved in 5'-end resection of DNA during
CC double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.
CC {ECO:0000256|RuleBase:RU367041}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU367041};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU367041}.
CC Chromosome {ECO:0000256|RuleBase:RU367041}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913, ECO:0000256|RuleBase:RU367041}.
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DR EMBL; CM022216; KAF7018337.1; -; Genomic_DNA.
DR EMBL; LS480641; SPT19893.1; -; Genomic_DNA.
DR SMR; A0A1D5UJQ1; -.
DR STRING; 4565.A0A1D5UJQ1; -.
DR EnsemblPlants; TraesCS2D02G451700.1; TraesCS2D02G451700.1; TraesCS2D02G451700.
DR Gramene; TraesCAD_scaffold_009397_01G000300.1; TraesCAD_scaffold_009397_01G000300.1; TraesCAD_scaffold_009397_01G000300.
DR Gramene; TraesCLE_scaffold_011844_01G000300.1; TraesCLE_scaffold_011844_01G000300.1; TraesCLE_scaffold_011844_01G000300.
DR Gramene; TraesCS2D02G451700.1; TraesCS2D02G451700.1; TraesCS2D02G451700.
DR Gramene; TraesCS2D03G1012800.1; TraesCS2D03G1012800.1.CDS; TraesCS2D03G1012800.
DR Gramene; TraesKAR2D01G0406780.1; cds.TraesKAR2D01G0406780.1; TraesKAR2D01G0406780.
DR Gramene; TraesPAR_scaffold_007792_01G000300.1; TraesPAR_scaffold_007792_01G000300.1; TraesPAR_scaffold_007792_01G000300.
DR Gramene; TraesROB_scaffold_006457_01G000400.1; TraesROB_scaffold_006457_01G000400.1; TraesROB_scaffold_006457_01G000400.
DR Gramene; TraesWEE_scaffold_046775_01G000100.1; TraesWEE_scaffold_046775_01G000100.1; TraesWEE_scaffold_046775_01G000100.
DR OMA; EENIWCT; -.
DR OrthoDB; 170190at2759; -.
DR Proteomes; UP000019116; Chromosome 2D.
DR Proteomes; UP000280104; Chromosome ii.
DR Proteomes; UP000815260; Chromosome 2D.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0017116; F:single-stranded DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:UniProtKB-UniRule.
DR GO; GO:0071932; P:replication fork reversal; IBA:GO_Central.
DR CDD; cd18041; DEXXQc_DNA2; 1.
DR CDD; cd22318; DNA2_N-like; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR Gene3D; 2.40.30.270; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR026851; Dna2/JHS1_DEXXQ-box.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR014808; DNA_replication_fac_Dna2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR047187; SF1_C_Upf1.
DR PANTHER; PTHR10887:SF433; DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2; 1.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF08696; Dna2; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|RuleBase:RU367041};
KW ATP-binding {ECO:0000256|RuleBase:RU367041};
KW Chromosome {ECO:0000256|RuleBase:RU367041};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU367041};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU367041};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU367041};
KW DNA-binding {ECO:0000256|RuleBase:RU367041};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|RuleBase:RU367041};
KW Hydrolase {ECO:0000256|RuleBase:RU367041};
KW Iron {ECO:0000256|RuleBase:RU367041};
KW Iron-sulfur {ECO:0000256|RuleBase:RU367041};
KW Metal-binding {ECO:0000256|RuleBase:RU367041};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268,
KW ECO:0000256|RuleBase:RU367041}; Nuclease {ECO:0000256|RuleBase:RU367041};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU367041};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU367041};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116}.
FT DOMAIN 306..503
FT /note="DNA replication factor Dna2 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08696"
FT DOMAIN 864..954
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 964..1031
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1040..1239
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
FT REGION 1..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..69
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1283 AA; 141490 MW; 8D3EB8D8099B8E91 CRC64;
MAPRKRAAAA AASKKPPPSQ PSQPPAKFGI LHFFERHQSQ AASQNPKRQK PVANPDPMPP
PPPPPEEELS EVSPEVTKTL APKRVKFSPG MLIKQSQDDG GGEVVTWKIS PVNDRLRTRQ
LPGMALHPCS NTEKHSSLEA MKKWHSSPLG LSRCTASARN LSVCGIGPGG GDGMEDQSPF
RTPPSLPYSF TEPLNAGITC DGGPDQLEAG QHKKALLDLL DQVEDAITEE ELPVDAGNKG
GQVTVGDSTD SRCSPIANSS LNVESNKPRE APSFDSFLVL EVSEKHKGDS SCDRYPAKVL
RLLNEHCGKE YSVHLCDEWF HSTVVPGDTV SVIGEFSDQG KCTVDHDSNL VIVHPELLIS
GTRVASSFHC PRRSVLDERL KSNEYSTSAL MGTLLHQVFQ AGLLEDVPSR QFLEQHAKEV
LLNNLESLYA CGASERSTHS ILIEAIPKML NWYKSFMKGS KSTNVDFGHT EGRKTVEVTE
MMDIEEMAWA PRYGLKGIID ASVISRVNSC GGGSYDKVMP LEFKTGKSTS GQSAMEHSAQ
VILYTLLMSE RYLNTDIDMG LLYYLHTDQT LGIKVKRSDL IGLMMRRNEL ASEILKASFS
QSFPAMLQSP SSCTGCRHLT SCTIYHKVHG GNTATSGLGD LFDNLVNHLS VAHHNFLKHW
DRLIDLEART SQVKKKEILL PLHYNSGSKS SAPSFYVLDM KNEHSVDSSG KSKRYIYNFV
REKMQPEAAG HSEPQAESLD FNLKSGDCVV LSTQSGRIAV ANGSIRDISR SHITVSLSRR
LRLPGSSSLL EQGDLQRELW RIDKDEFSSS FATMRFNLVQ LFSQKPQNTK LRKLVVDLEA
PRFDSGGLFS QDPALSYIQS LTSLNNDQQR SLHKILAAKD YALILGMPGT GKTYTMVHAV
KSLLMRGESI LLTSYTNSAI DNLLMKLKAE GVDFIRIGRH EAVHPDVRAN CLSTTEVQSV
DAIKTRMEQV QVVGVTCLGV YHPLLAHKKF DTCIMDEAGQ ITLPVSLGPL MLATKFVLVG
DHYQLPPLIQ SFEARESGMG ISLFWRLSEA HPQAISALRC QYRMSSGIME LSNSLIYGNR
LCCGSLEIAN AKLKFSGKQQ VHFKFKEILN PNRAVIFANT DQVPALEAKE HRTVNNPTEA
YIVSWITKQL LKRGVAQDEI GIITPYNAQV NLIRQQVDPL VEVHTIDKYQ GRDKDCIIVS
FVRSSGNSRA SGSSLLGDWH RINVVLTRAK KKLIMVGSAG TLSTIPLLRL LVEKVAEGGG
QLDLTNKDVQ SIPELRNSQL NVQ
//
