ID A0A1D5UUX8_WHEAT Unreviewed; 359 AA.
AC A0A1D5UUX8;
DT 30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=cinnamyl-alcohol dehydrogenase {ECO:0000256|ARBA:ARBA00013171};
DE EC=1.1.1.195 {ECO:0000256|ARBA:ARBA00013171};
GN ORFNames=CAMPLR22A2D_LOCUS580 {ECO:0000313|EMBL:SPT15987.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS2D02G069800.1};
RN [1] {ECO:0000313|EnsemblPlants:TraesCS2D02G069800.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS2D02G069800.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [2] {ECO:0000313|EMBL:SPT15987.1, ECO:0000313|Proteomes:UP000280104}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Thind KAUR A.;
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:TraesCS2D02G069800.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-4-coumaroyl alcohol + NADP(+) = (E)-4-coumaraldehyde +
CC H(+) + NADPH; Xref=Rhea:RHEA:45724, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28353, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:64555; EC=1.1.1.195;
CC Evidence={ECO:0000256|ARBA:ARBA00036200};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:45726;
CC Evidence={ECO:0000256|ARBA:ARBA00036200};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-caffeyl alcohol + NADP(+) = (E)-caffeyl aldehyde + H(+) +
CC NADPH; Xref=Rhea:RHEA:45728, ChEBI:CHEBI:15378, ChEBI:CHEBI:28323,
CC ChEBI:CHEBI:31334, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC Evidence={ECO:0000256|ARBA:ARBA00035979};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:45730;
CC Evidence={ECO:0000256|ARBA:ARBA00035979};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-cinnamyl alcohol + NADP(+) = (E)-cinnamaldehyde + H(+) +
CC NADPH; Xref=Rhea:RHEA:10392, ChEBI:CHEBI:15378, ChEBI:CHEBI:16731,
CC ChEBI:CHEBI:33227, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.195; Evidence={ECO:0000256|ARBA:ARBA00036166};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:10394;
CC Evidence={ECO:0000256|ARBA:ARBA00036166};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-coniferol + NADP(+) = (E)-coniferaldehyde + H(+) + NADPH;
CC Xref=Rhea:RHEA:22444, ChEBI:CHEBI:15378, ChEBI:CHEBI:16547,
CC ChEBI:CHEBI:17745, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.195; Evidence={ECO:0000256|ARBA:ARBA00036509};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:22446;
CC Evidence={ECO:0000256|ARBA:ARBA00036509};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(E)-sinapyl alcohol + NADP(+) = (E)-sinapaldehyde + H(+) +
CC NADPH; Xref=Rhea:RHEA:45704, ChEBI:CHEBI:15378, ChEBI:CHEBI:27949,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:64557;
CC EC=1.1.1.195; Evidence={ECO:0000256|ARBA:ARBA00035824};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:45706;
CC Evidence={ECO:0000256|ARBA:ARBA00035824};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947,
CC ECO:0000256|RuleBase:RU361277};
CC -!- PATHWAY: Aromatic compound metabolism; phenylpropanoid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004928}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC family. {ECO:0000256|RuleBase:RU361277}.
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DR EMBL; LS480641; SPT15987.1; -; Genomic_DNA.
DR SMR; A0A1D5UUX8; -.
DR STRING; 4565.A0A1D5UUX8; -.
DR EnsemblPlants; TraesCS2D02G069800.1; TraesCS2D02G069800.1; TraesCS2D02G069800.
DR Gramene; TraesCAD_scaffold_006715_01G000100.1; TraesCAD_scaffold_006715_01G000100.1; TraesCAD_scaffold_006715_01G000100.
DR Gramene; TraesCLE_scaffold_004173_01G000600.1; TraesCLE_scaffold_004173_01G000600.1; TraesCLE_scaffold_004173_01G000600.
DR Gramene; TraesCS2D02G069800.1; TraesCS2D02G069800.1; TraesCS2D02G069800.
DR Gramene; TraesCS2D03G0136600.1; TraesCS2D03G0136600.1.CDS; TraesCS2D03G0136600.
DR Gramene; TraesKAR2D01G0026160.1; cds.TraesKAR2D01G0026160.1; TraesKAR2D01G0026160.
DR Gramene; TraesPAR_scaffold_006037_01G000400.1; TraesPAR_scaffold_006037_01G000400.1; TraesPAR_scaffold_006037_01G000400.
DR Gramene; TraesRN2D0100152600.1; TraesRN2D0100152600.1; TraesRN2D0100152600.
DR Gramene; TraesROB_scaffold_002744_01G000600.1; TraesROB_scaffold_002744_01G000600.1; TraesROB_scaffold_002744_01G000600.
DR Gramene; TraesWEE_scaffold_002999_01G000600.1; TraesWEE_scaffold_002999_01G000600.1; TraesWEE_scaffold_002999_01G000600.
DR OMA; EQCDISQ; -.
DR OrthoDB; 1550at2759; -.
DR Proteomes; UP000019116; Chromosome 2D.
DR Proteomes; UP000280104; Chromosome ii.
DR GO; GO:0045551; F:cinnamyl-alcohol dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009809; P:lignin biosynthetic process; IBA:GO_Central.
DR CDD; cd05283; CAD1; 1.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR013149; ADH-like_C.
DR InterPro; IPR013154; ADH-like_N.
DR InterPro; IPR002328; ADH_Zn_CS.
DR InterPro; IPR047109; CAD-like.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020843; PKS_ER.
DR PANTHER; PTHR42683; ALDEHYDE REDUCTASE; 1.
DR PANTHER; PTHR42683:SF91; GENOME ASSEMBLY, CHROMOSOME: II; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF00107; ADH_zinc_N; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SUPFAM; SSF50129; GroES-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00059; ADH_ZINC; 1.
PE 3: Inferred from homology;
KW Lignin biosynthesis {ECO:0000256|ARBA:ARBA00022733};
KW Metal-binding {ECO:0000256|RuleBase:RU361277};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU361277}.
FT DOMAIN 16..350
FT /note="Enoyl reductase (ER)"
FT /evidence="ECO:0000259|SMART:SM00829"
SQ SEQUENCE 359 AA; 38828 MW; A1AF244B18F3D8DE CRC64;
MEVTPNHTQE VSGWAAMDES GKMVPFNFKR RENGVDDVTI KVLYCGMCHT DLHFAKNHWG
ITTYPVVPGH EITGVVTKVG ANVSGFRPGD RVGVGCLACS CLDCEQCDIS QENYCDKMGL
TYNSVYWDGS ITYGGYSSMY VAHKRFVVRV PDSLPLDAAA PLLCAGITVY TPMKKHGMLR
AAGRRLGVVG LGGLGHIAVK FGKAFGLHVT VISTSPAKEQ EARENLKADD FIISTDDKQM
QAMARKLDYV IDTVPAAHSL GPILELLKVG GALALVAAPD GPLELPSFPL IFGNKTISGS
ITGGMNDHQE MMDLCGEHNI TCDIEIVSND GINNALARLA RNDVRYRFVI DIVGGDSRF
//
