ID A0A1D6EKP0_MAIZE Unreviewed; 525 AA.
AC A0A1D6EKP0;
DT 30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2016, sequence version 1.
DT 22-FEB-2023, entry version 34.
DE RecName: Full=Glutamine-dependent NAD(+) synthetase {ECO:0000256|PIRNR:PIRNR006630};
DE EC=6.3.5.1 {ECO:0000256|PIRNR:PIRNR006630};
DE AltName: Full=NAD(+) synthase [glutamine-hydrolyzing] {ECO:0000256|PIRNR:PIRNR006630};
GN ORFNames=ZEAMMB73_Zm00001d005157 {ECO:0000313|EMBL:ONM20479.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577 {ECO:0000313|EMBL:ONM20479.1};
RN [1] {ECO:0000313|EMBL:ONM20479.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Seedling {ECO:0000313|EMBL:ONM20479.1};
RG Maize Genome Sequencing Project;
RA Ware D.;
RT "Update maize B73 reference genome by single molecule sequencing
RT technologies.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate +
CC H(+) + L-glutamate + NAD(+); Xref=Rhea:RHEA:24384, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, ChEBI:CHEBI:58359,
CC ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.5.1;
CC Evidence={ECO:0000256|PIRNR:PIRNR006630};
CC -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC deamido-NAD(+) (L-Gln route): step 1/1. {ECO:0000256|ARBA:ARBA00005188,
CC ECO:0000256|PIRNR:PIRNR006630}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the NAD synthetase
CC family. {ECO:0000256|ARBA:ARBA00007145, ECO:0000256|PIRNR:PIRNR006630}.
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DR EMBL; CM007648; ONM20477.1; -; Genomic_DNA.
DR EMBL; CM007648; ONM20479.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1D6EKP0; -.
DR UniPathway; UPA00253; UER00334.
DR ExpressionAtlas; A0A1D6EKP0; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004359; F:glutaminase activity; IEA:InterPro.
DR GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009435; P:NAD biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd07570; GAT_Gln-NAD-synth; 1.
DR Gene3D; 3.60.110.10; Carbon-nitrogen hydrolase; 2.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR014445; Gln-dep_NAD_synthase.
DR InterPro; IPR022310; NAD/GMP_synthase.
DR InterPro; IPR003694; NAD_synthase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR23090:SF9; GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE; 1.
DR PANTHER; PTHR23090; NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE; 1.
DR Pfam; PF00795; CN_hydrolase; 1.
DR Pfam; PF02540; NAD_synthase; 1.
DR PIRSF; PIRSF006630; NADS_GAT; 2.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF56317; Carbon-nitrogen hydrolase; 1.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR006630};
KW Ligase {ECO:0000256|PIRNR:PIRNR006630};
KW NAD {ECO:0000256|PIRNR:PIRNR006630};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR006630}.
FT DOMAIN 4..303
FT /note="CN hydrolase"
FT /evidence="ECO:0000259|PROSITE:PS50263"
SQ SEQUENCE 525 AA; 58966 MW; 1777A15524ADF177 CRC64;
MRLLRVATCN LNQWAMDFDT NLRNVKESIK RAKAAGAVIR VGPELELTGY GCEDHFLEQD
TTAHAWECLK DILTGDYTDN ILCSIGMPVI FNSVRYNCQV FCLNRKIIMI RPKMSLANDG
NYREFRWFSA WTFKGEIVDF QLPVEVSEAI SQDTVPFGYG YMRFIDVSLA AETCEELFTA
NAPRIDLALN GVEVFMNASG SHHQLRKLNL RIDSIRSATQ TCGGVYMYAN QQGCDGGRLY
YGISKCHDVL TMYAVFHFKR NLLFSNIIPL DGCCCIAVNG DLVGQGSQFS LKDVEVLDAL
VDLDAVSSYR ASVSSFREQA SHKTNVPFVK VPYKLCQPFR SGMVPTSPVE IMYHCPEEEI
AFGPSCWLWD YLRRSQASGF LLPLSGGADS SSVAAIVGCM CQLVIKDIEK GDEQVKADAL
RIGQYKDGAI PMDSRELAKR LFYTVYMGTE NSSEDTRSRA KRLAEEIGSF HLNVPIDSIV
SAFLSLFETL TGKRPRYKVF LQHDITLLDY CNAANIKKLE QPRRN
//
