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Database: UniProt
Entry: A0A1D6FAC8_MAIZE
LinkDB: A0A1D6FAC8_MAIZE
Original site: A0A1D6FAC8_MAIZE 
ID   A0A1D6FAC8_MAIZE        Unreviewed;       846 AA.
AC   A0A1D6FAC8;
DT   30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   30-NOV-2016, sequence version 1.
DT   18-SEP-2019, entry version 23.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   Name=100277946 {ECO:0000313|EnsemblPlants:Zm00001d007945_P004};
GN   ORFNames=ZEAMMB73_Zm00001d007945 {ECO:0000313|EMBL:ONM28030.1};
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae;
OC   PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae;
OC   Zea.
OX   NCBI_TaxID=4577 {ECO:0000313|EnsemblPlants:Zm00001d007945_P004, ECO:0000313|Proteomes:UP000007305};
RN   [1] {ECO:0000313|EMBL:ONM28030.1, ECO:0000313|EnsemblPlants:Zm00001d007945_P004, ECO:0000313|Proteomes:UP000007305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. B73 {ECO:0000313|EnsemblPlants:Zm00001d007945_P004,
RC   ECO:0000313|Proteomes:UP000007305};
RC   TISSUE=Seedling {ECO:0000313|EMBL:ONM28030.1};
RG   Maize Genome Sequencing Project;
RA   Ware D.;
RT   "Update maize B73 reference genome by single molecule sequencing
RT   technologies.";
RL   Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblPlants:Zm00001d007945_P004}
RP   IDENTIFICATION.
RC   STRAIN=cv. B73 {ECO:0000313|EnsemblPlants:Zm00001d007945_P004};
RG   EnsemblPlants;
RL   Submitted (MAY-2017) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation
CC       of feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00700}.
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DR   EMBL; CM007648; ONM28030.1; -; Genomic_DNA.
DR   EnsemblPlants; Zm00001d007945_T004; Zm00001d007945_P004; Zm00001d007945.
DR   Gramene; Zm00001d007945_T004; Zm00001d007945_P004; Zm00001d007945.
DR   OrthoDB; 183108at2759; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000007305; Chromosome 2.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 2.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 2.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PIRSF; PIRSF001222; Urease; 3.
DR   SUPFAM; SSF51278; SSF51278; 2.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000007305};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222};
KW   Reference proteome {ECO:0000313|Proteomes:UP000007305}.
FT   DOMAIN      456    846       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   BINDING     546    546       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     544    544       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   846 AA;  91166 MW;  31C33A57EB6EBBBF CRC64;
     MRLLPREADK LALHNAGFLA QKRLARGLRL NYTEAVALIA AQILELIRDG DKTVTDLMDL
     GKQLLGRRQV LPAVPYLLHT VQVEGTFVDG TKLVTVHDPI SLDDGNLELA LHGSFLPVPS
     PEKFSSDDVE EYPGEIHYSS SRIVLNLHRR ALSLKVVNKA DRPIQVSTPG YFCLDPENIC
     PVPPCYVELV RVFQTTCRCK RFFLNYKPLV TLMKISKYCL ILQIGSHYHF IETNPYLVFD
     RKRAYGMRLN ILAGTAVRFE PGDAKSVILV SIGGHKVIKG GNGIADGPID SSSLNVVMQK
     VNANSFGHED YPDAREGIIG DGSFDCTVDH EKYASIYGPT TGDKIRLGDT NLYAEIEKDF
     AFYGDECIFG GGKVLRDGMG QASGYPESFC LDTVITNAVV IDYTGIYKAD IGIKGGLIVA
     IGKAGNPDVM DGVHNNMIVG VNTEVIASEG MIVTAGGIDC HVHFICPQLA EEAIASGITT
     LVGGGTGPAH GTCATTCTPA PSQLKLMLQS TDQLPINMGF TGKGNTSKPE GLAEIIKAGA
     MGLKLHEDWG TTPSAIDNCL SVAEDFDIQV NIHTDTLNES GCVEHTIAAF KGRAIHTYHS
     EGAGGGHAPD IIKVCGVKNV LPSSTNPTRP FTSNTVDEHL DMLVITRTWQ TANKMKVQRG
     SLPGSGDANA APDSDNLRIR RYIAKYTINP AIVNGFSDFV GSVEVGKLAD LVLWKPSFFG
     AKPELVVKGG AIAWANMGDP NASIPTPEPV VMRPMFGAFG KAGSSNSIAF VSKAAKEAGV
     ATEYRLEKRV EAVGRVRGLT KLDMKLNDAL PKIEVDPETY TVTADGEVLT CQPAPTLPLS
     RNYFLF
//
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