ID A0A1D6HSV7_MAIZE Unreviewed; 573 AA.
AC A0A1D6HSV7;
DT 30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Small RNA 2'-O-methyltransferase {ECO:0000256|ARBA:ARBA00021330};
DE EC=2.1.1.386 {ECO:0000256|ARBA:ARBA00035025};
GN ORFNames=ZEAMMB73_Zm00001d018863 {ECO:0000313|EMBL:ONM51480.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577 {ECO:0000313|EMBL:ONM51480.1};
RN [1] {ECO:0000313|EMBL:ONM51480.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Seedling {ECO:0000313|EMBL:ONM51480.1};
RG Maize Genome Sequencing Project;
RA Ware D.;
RT "Update maize B73 reference genome by single molecule sequencing
RT technologies.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H(+) +
CC S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotide;
CC Xref=Rhea:RHEA:37887, Rhea:RHEA-COMP:10415, Rhea:RHEA-COMP:10416,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74896, ChEBI:CHEBI:74898; EC=2.1.1.386;
CC Evidence={ECO:0000256|ARBA:ARBA00034992};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. HEN1 family.
CC {ECO:0000256|ARBA:ARBA00009026}.
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DR EMBL; CM007650; ONM51480.1; -; Genomic_DNA.
DR EMBL; CM007650; ONM51482.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1D6HSV7; -.
DR OMA; FRSHEHK; -.
DR ExpressionAtlas; A0A1D6HSV7; baseline and differential.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProtKB-KW.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR026610; Hen1.
DR InterPro; IPR040870; HEN1_dsRBD2.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR21404; HEN1; 1.
DR PANTHER; PTHR21404:SF3; SMALL RNA 2'-O-METHYLTRANSFERASE; 1.
DR Pfam; PF17842; dsRBD2; 1.
DR Pfam; PF08242; Methyltransf_12; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW RNA-mediated gene silencing {ECO:0000256|ARBA:ARBA00023158};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..132
FT /note="HEN1 double-stranded RNA binding"
FT /evidence="ECO:0000259|Pfam:PF17842"
FT DOMAIN 350..444
FT /note="Methyltransferase type 12"
FT /evidence="ECO:0000259|Pfam:PF08242"
SQ SEQUENCE 573 AA; 63504 MW; 821E8AC15CDA5B99 CRC64;
MSRDSILAAE LPSAYSRFSW KGLSPRDLLC SFCRLQRLPE PHFAVSRVSC DTLMSAVSSE
ERGAPAGSAE NQYTNDVRIN KEKPDLFKCE VNIRSRKQEV LLEYSAADTW SKESDAIQNS
SLKVLIWFNN YFKKLTSKTG KLYLSESTAG VKVHSNIFLQ EFAMCLSVYG KNGCSDSAMC
SAVGPFSMDT QKKHLESTAK LTHIEGPDSG VFPSHGSLTC ISYTASLVTK DKAKTYLLES
HNEFEFEIGT GAVSNQLESC VTQLSVNQTA RFVAELPPRD LIMAASCEFS HDLSNVSSES
CFLEFSVKVL QVTEPLEDRM EKALFSPPLS KQRVEFAVRH INELHATTLV DFGCGSGSLL
DSLLEHPTTL EKLVGVDISR KGLTRAAKSL HQKLSKKSTT QTNVPTAVLY DGSITDYDSR
LYGFDIGTCL EVIEHVEEDQ ATLFGNVVLS SFRPTVLIVS TPNYEYNPIL QGSAMPNKDD
EADESAGPCK FRNHDHKFEW TRLQFQCWAT GLAVKHSYSV EFSGVGGSGE EPGYASQIAV
FRRLVQGQEA MCLDEDGDQP YEVLWEWPNA CVN
//
