ID   A0A1D6KMX1_MAIZE        Unreviewed;       638 AA.
AC   A0A1D6KMX1;
DT   30-NOV-2016, integrated into UniProtKB/TrEMBL.
DT   30-NOV-2016, sequence version 1.
DT   27-MAR-2024, entry version 33.
DE   RecName: Full=rhamnogalacturonan endolyase {ECO:0000256|ARBA:ARBA00012437};
DE            EC=4.2.2.23 {ECO:0000256|ARBA:ARBA00012437};
GN   ORFNames=ZEAMMB73_Zm00001d031993 {ECO:0000313|EMBL:ONM04178.1};
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577 {ECO:0000313|EMBL:ONM04178.1};
RN   [1] {ECO:0000313|EMBL:ONM04178.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Seedling {ECO:0000313|EMBL:ONM04178.1};
RG   Maize Genome Sequencing Project;
RA   Ware D.;
RT   "Update maize B73 reference genome by single molecule sequencing
RT   technologies.";
RL   Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-
CC         (1->4)-alpha-D-galactopyranosyluronic acid bonds of
CC         rhamnogalacturonan I domains in ramified hairy regions of pectin
CC         leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-
CC         unsaturated D-galactopyranosyluronic acid at the non-reducing end.;
CC         EC=4.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001324};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 4 family.
CC       {ECO:0000256|ARBA:ARBA00010418}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM007647; ONM04178.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1D6KMX1; -.
DR   IntAct; A0A1D6KMX1; 17.
DR   STRING; 4577.A0A1D6KMX1; -.
DR   PaxDb; 4577-GRMZM2G154124_P01; -.
DR   eggNOG; ENOG502QQM5; Eukaryota.
DR   InParanoid; A0A1D6KMX1; -.
DR   OMA; PQFYLFN; -.
DR   ExpressionAtlas; A0A1D6KMX1; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0102210; F:rhamnogalacturonan endolyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR   CDD; cd10317; RGL4_C; 1.
DR   CDD; cd10316; RGL4_M; 1.
DR   CDD; cd10320; RGL4_N; 1.
DR   Gene3D; 2.70.98.10; -; 1.
DR   Gene3D; 2.60.40.1120; Carboxypeptidase-like, regulatory domain; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   InterPro; IPR013784; Carb-bd-like_fold.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR029413; RG-lyase_II.
DR   InterPro; IPR029411; RG-lyase_III.
DR   InterPro; IPR010325; Rhamnogal_lyase.
DR   PANTHER; PTHR32018:SF1; RHAMNOGALACTURONAN ENDOLYASE; 1.
DR   PANTHER; PTHR32018; RHAMNOGALACTURONATE LYASE FAMILY PROTEIN; 1.
DR   Pfam; PF14683; CBM-like; 1.
DR   Pfam; PF14686; fn3_3; 1.
DR   Pfam; PF06045; Rhamnogal_lyase; 1.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:ONM04178.1};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT   DOMAIN          357..428
FT                   /note="Rhamnogalacturonan lyase"
FT                   /evidence="ECO:0000259|Pfam:PF14686"
FT   DOMAIN          443..632
FT                   /note="Rhamnogalacturonan lyase"
FT                   /evidence="ECO:0000259|Pfam:PF14683"
SQ   SEQUENCE   638 AA;  72962 MW;  D9A65D9A9851B211 CRC64;
     MGDTLIRPSG RKPHAQVEIK NGIFDLTLSN PDGIVTGVRY NGVDNLMEIL NKEDNRGYWD
     LVWNPPGQKT GIFDVIKGTE FRIVYQDENQ AEVSFTRNWD PSLEGKAVPL NIDKRFIVLR
     GSSGFYTYGI YEHKEGWPDF GLGETRVAFK LRKDMFHYMA LADDRQRIMP MPDDRLPPRG
     QPLAYPEAVL LVDPINPELR GEVDDKYQYS CEDQYNNVHG WMSFDPPIGF WQITPSDEFR
     TGGPLKQNLT SHVGPTMLAM FLSAHYAGDD LSPKFTNGEY WKKVHGPVFM YLNSSWDASD
     PTMLWEDAKV QMMIEKGNWP YCFALSEDFQ KTEQRGCVSG RLLVRDRYID DQDLYASGAY
     VGLALPGEAG SWQRECKGYQ FWCRADVDGS FYIRNIVTGN YNLYAWVPGF LGDYKLDATV
     TIASGDDIYL GDLVYEPPRD GPTMWEIGVP DRSAAEFYVP DPNPNYVNRL YVNHPADRFR
     QYGLWERYAE LYPDSDLVYT VGQSDYSTDW FYAQVNRKID DNTYQPTTWQ IKFDLDSVSP
     SSTYKFRVAL ASSALAELQI SFNDQDRAVP HFATGLIGRD NAIARHGIHG LYWLFNIDVA
     SAWLVQGVNT IYLKQPRSQS PFQGLMYDYL RLEGPCGC
//