ID A0A1D8G1H1_9ACTN Unreviewed; 1176 AA.
AC A0A1D8G1H1;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:AOT59300.1};
GN ORFNames=A4G23_02137 {ECO:0000313|EMBL:AOT59300.1};
OS Streptomyces rubrolavendulae.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=285473 {ECO:0000313|EMBL:AOT59300.1, ECO:0000313|Proteomes:UP000095349};
RN [1] {ECO:0000313|EMBL:AOT59300.1, ECO:0000313|Proteomes:UP000095349}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MJM4426 {ECO:0000313|EMBL:AOT59300.1,
RC ECO:0000313|Proteomes:UP000095349};
RA Kim J.-G.;
RT "Streptomyces rubrolavendulae MJM4426 Genome sequencing and assembly.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; CP017316; AOT59300.1; -; Genomic_DNA.
DR RefSeq; WP_031133197.1; NZ_CP017316.1.
DR AlphaFoldDB; A0A1D8G1H1; -.
DR STRING; 285473.A4G23_02137; -.
DR KEGG; srn:A4G23_02137; -.
DR PATRIC; fig|285473.5.peg.2228; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000095349; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000095349}.
FT DOMAIN 641..802
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 827..977
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1176 AA; 128371 MW; EC3B5A3AFE07F3D2 CRC64;
MSLHGLLDAV VKDPALAEAV KAAADGRRAH VDLVGPPAAR PFAVAALARD AGRPVLAVTA
TGREAEDLAA ALRSLLDPDR VVEYPSWETL PHERLSPRSD TVGRRLAVLR RLAHPSPDDP
AAGPVQVVVA PVRSVLQPQV KGLGDLEPVS LRTGQSADLG AVVEGLAAAA YQRVELVEKR
GEFAVRGGIL DVFPPTEEHP VRIEFWGDDV EEIRYFKVAD QRSLEVAEHG LWAPPCRELL
LTPQVRERAA ALAEAHPELG ELLGKIAEGI AVEGMESLAP VLVDDMELLL DVLPQGSMAV
VCDPERVRTR SADLVATSQE FLQASWAATA GGGEAPIDVG AASLWSIADV RDRARELGMP
WWTVSPFAPD AELEADTIQL GMHAPEAYRG DTARALADTK GWLADGWRTV YVTEAHGPAA
RTVEVLGGEG IAARLEGELG DIAPSVVHVA CGSVEHGFVD EGLKLAVLTE TDLTGQKAAG
KDGRRMPTRR RKTIDPLTLE AGDYIVHEQH GVGRYIEMVQ RTVQGATREY LLVEYAPAKR
GQPGDRLYIP TDQLEQVTKY VGGEAPTLHR LGGADWTKTK QRAKKAVKEI AADLIKLYSA
RMAAPGHTFG PDTPWQRELE DAFPYAETPD QLSTIAEVKE DMEKSVPMDR LICGDVGYGK
TEIAVRAAFK AVQDGKQVAV LVPTTLLVQQ HFGTFSERYA QFPVVVKALS RFQSDAEAKA
TLEGLREGSV DVVIGTHRLF SSETRFKDLG LVIVDEEQRF GVEHKEQLKK LRANVDVLTM
SATPIPRTLE MAVTGIREMS TITTPPEERH PVLTFVGPYE ERQIGAAIRR ELLREGQVFY
IHNRVESIDR AAARLREIVP EARIATAHGQ MSEQALEQVV VDFWEKKFDV LVSTTIVESG
IDISNANTLI VERGDNFGLS QLHQLRGRVG RGRERGYAYF LYPPEKPLTE TAHERLATIA
QHTEMGAGMY VAMKDLEIRG AGNLLGGEQS GHIAGVGFDL YVRMVGEAVA DYRASLEGGR
EEEPPLEVKI ELPVDAHVPH DYAPGERLRL QAYRAIASAN SEEDIRAVRE ELTDRYGKLP
EPVENLLLVA GLRMLARACG VGEIVLQGAN IRFAPVELRE SQELRLKRLY PRTVIKAPAR
QILVPRPTAG TIGGKPVVGR DLLAWTGEFL TTILGS
//
