ID A0A1D8G5E2_9ACTN Unreviewed; 530 AA.
AC A0A1D8G5E2;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Methionyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030904};
GN Name=metG_2 {ECO:0000313|EMBL:AOT60670.1};
GN ORFNames=A4G23_03545 {ECO:0000313|EMBL:AOT60670.1};
OS Streptomyces rubrolavendulae.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=285473 {ECO:0000313|EMBL:AOT60670.1, ECO:0000313|Proteomes:UP000095349};
RN [1] {ECO:0000313|EMBL:AOT60670.1, ECO:0000313|Proteomes:UP000095349}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MJM4426 {ECO:0000313|EMBL:AOT60670.1,
RC ECO:0000313|Proteomes:UP000095349};
RA Kim J.-G.;
RT "Streptomyces rubrolavendulae MJM4426 Genome sequencing and assembly.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10;
CC Evidence={ECO:0000256|ARBA:ARBA00001234};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|RuleBase:RU363039}.
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DR EMBL; CP017316; AOT60670.1; -; Genomic_DNA.
DR RefSeq; WP_069977753.1; NZ_CP017316.1.
DR AlphaFoldDB; A0A1D8G5E2; -.
DR STRING; 285473.A4G23_03545; -.
DR KEGG; srn:A4G23_03545; -.
DR PATRIC; fig|285473.5.peg.3713; -.
DR OrthoDB; 9810191at2; -.
DR Proteomes; UP000095349; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 2.20.28.20; Methionyl-tRNA synthetase, Zn-domain; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR023458; Met-tRNA_ligase_1.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR029038; MetRS_Zn.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR45765; METHIONINE--TRNA LIGASE; 1.
DR PANTHER; PTHR45765:SF1; METHIONINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363039};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363039};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363039};
KW Reference proteome {ECO:0000313|Proteomes:UP000095349}.
FT DOMAIN 6..368
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
SQ SEQUENCE 530 AA; 57422 MW; 63E621B951212D7E CRC64;
MTTRTWITAT PPTPNGDLHV GHLAGPYTAA DVLRRFLTAD GADVRLTTGL DDHQGYVAVR
GIKDGGRKPE EVADHYGASV TRTWKAAAVE FDHIVRPRRD AGYQQFVQSF FSGLYEAGHI
VPRTRPLPYC ASCERWLYEG YLVGGCPLCG ASSNGNACEP CGRPNECGDL TDPRCVPCGA
PAELRDATRL YFPLAPFAER LAAFWAEVDM PPHLRALCER MLADGLPEIA VSHPADWGVP
VPVDGYEDQR IFVWCEMAPG YLWEQDPRGG TLPETGPVQF FGFDNGYFHA VLFPALFLAH
DEATPLPRAF VVNEFYQLDG LKFSTSRRHA VWVHEALAAC GADVLRHHVL ADRPNGRETS
FNLEALERTR HHLATLWDGW LGRLLTAVER DCGGRVPEEA PAGRAWELLR GRLHRIADEL
REAYGVTGFD PRRAVALLDE AVACAADFGH VHAHERARPD GAAAHRRALA AQLAVAAALS
AWAAPALPVG SARLAALLGV PANGRVDASA LAPLPAGRAV TAPTGAVFGG
//
