ID A0A1D8GEB4_9CLOT Unreviewed; 558 AA.
AC A0A1D8GEB4;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN ORFNames=Gferi_06365 {ECO:0000313|EMBL:AOT69220.1};
OS Geosporobacter ferrireducens.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Geosporobacter.
OX NCBI_TaxID=1424294 {ECO:0000313|EMBL:AOT69220.1, ECO:0000313|Proteomes:UP000095743};
RN [1] {ECO:0000313|EMBL:AOT69220.1, ECO:0000313|Proteomes:UP000095743}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IRF9 {ECO:0000313|EMBL:AOT69220.1,
RC ECO:0000313|Proteomes:UP000095743};
RA Kim S.-J.;
RT "Genomic analysis reveals versatility of anaerobic energy metabolism of
RT Geosporobacter ferrireducens IRF9 of phylum Firmicutes.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; CP017269; AOT69220.1; -; Genomic_DNA.
DR RefSeq; WP_069974786.1; NZ_CP017269.1.
DR AlphaFoldDB; A0A1D8GEB4; -.
DR STRING; 1424294.Gferi_06365; -.
DR KEGG; gfe:Gferi_06365; -.
DR OrthoDB; 9761733at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000095743; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:AOT69220.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000095743}.
FT BINDING 66..73
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 558 AA; 59712 MW; 20E7AB5E91FA1DF3 CRC64;
MYKSDIEIAQ EAKLQDIREI AKKLNLTEAD LELYGKYKAK VDYNLLKKDN GGKKAKLILV
TAINPTPAGE GKTTTTIGTA DALSKLGKNT LVALREPSLG PVFGVKGGAA GGGYAQVVPM
EDINLHFTGD FHAIGAANNL LAAMIDNHIY QGNQLGIDAR KLTWRRAMDM NDRQLRNMVN
GLGGKGNGVP REDGFDITVA SEVMAAFCLA SDIMDLKERL GNIIVGYTKD DQPILARQLN
AHGAMAALLK DALKPNLVQT LEGTPAFVHG GPFANIAHGC NSVIATKMAA HFADYVVTEA
GFGADLGAEK FLDIKCRMAG LSPDAVIIVA TVRALKYNGG VSKSELNQEN LEALEKGLPN
LLKHVENITK VFNLPAVVAI NRFPLDTEAE LELVRRRCGE LGVNVALSEV WAKGGEGGQE
LAQEVLKLIE KGEANLTHPY ELDMPIKEKI RAIAQKVYGA DDVAFAGGAA KEIDRLEKLG
FGNLPICMAK TQYSLSDDPN KLGRPTGFTV TVRQVKVSAG AGFIVALTGE IMTMPGLPKV
PSAEKIDVDE NGVITGLF
//
