ID A0A1D8PGY1_CANAL Unreviewed; 627 AA.
AC A0A1D8PGY1;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 08-NOV-2023, entry version 28.
DE RecName: Full=Diphthine--ammonia ligase {ECO:0000256|ARBA:ARBA00018426};
DE EC=6.3.1.14 {ECO:0000256|ARBA:ARBA00012089};
DE AltName: Full=Diphthamide synthase {ECO:0000256|ARBA:ARBA00029814};
DE AltName: Full=Diphthamide synthetase {ECO:0000256|ARBA:ARBA00031552};
GN OrderedLocusNames=CAALFM_C203830WA {ECO:0000313|EMBL:AOW27412.1},
GN orf19.8458 {ECO:0000313|CGD:CAL0000190353};
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561 {ECO:0000313|EMBL:AOW27412.1, ECO:0000313|Proteomes:UP000000559};
RN [1] {ECO:0000313|EMBL:AOW27412.1, ECO:0000313|Proteomes:UP000000559}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876 {ECO:0000313|Proteomes:UP000000559};
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2] {ECO:0000313|EMBL:AOW27412.1, ECO:0000313|Proteomes:UP000000559}
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876 {ECO:0000313|Proteomes:UP000000559};
RX PubMed=17419877; DOI=.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3] {ECO:0000313|EMBL:AOW27412.1, ECO:0000313|Proteomes:UP000000559}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876 {ECO:0000313|Proteomes:UP000000559};
RX PubMed=24025428; DOI=.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + diphthine-[translation elongation factor 2] + NH4(+) =
CC AMP + diphosphate + diphthamide-[translation elongation factor 2] +
CC H(+); Xref=Rhea:RHEA:19753, Rhea:RHEA-COMP:10172, Rhea:RHEA-
CC COMP:10174, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:82696,
CC ChEBI:CHEBI:456215; EC=6.3.1.14;
CC Evidence={ECO:0000256|ARBA:ARBA00001559};
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DR EMBL; CP017624; AOW27412.1; -; Genomic_DNA.
DR RefSeq; XP_720963.2; XM_715870.2.
DR AlphaFoldDB; A0A1D8PGY1; -.
DR SMR; A0A1D8PGY1; -.
DR STRING; 237561.A0A1D8PGY1; -.
DR EnsemblFungi; C2_03830W_A-T; C2_03830W_A-T-p1; C2_03830W_A.
DR GeneID; 3637399; -.
DR KEGG; cal:CAALFM_C203830WA; -.
DR CGD; CAL0000190353; orf19.8458.
DR VEuPathDB; FungiDB:C2_03830W_A; -.
DR eggNOG; KOG2316; Eukaryota.
DR eggNOG; KOG2317; Eukaryota.
DR InParanoid; A0A1D8PGY1; -.
DR OrthoDB; 103959at2759; -.
DR Proteomes; UP000000559; Chromosome 2.
DR GO; GO:0017178; F:diphthine-ammonia ligase activity; IBA:GO_Central.
DR GO; GO:0017183; P:protein histidyl modification to diphthamide; IBA:GO_Central.
DR CDD; cd01994; Alpha_ANH_like_IV; 1.
DR CDD; cd06155; eu_AANH_C_1; 1.
DR CDD; cd06156; eu_AANH_C_2; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.90.1490.10; putative n-type atp pyrophosphatase, domain 2; 1.
DR Gene3D; 3.30.1330.40; RutC-like; 2.
DR InterPro; IPR002761; Diphthami_syn_dom.
DR InterPro; IPR030662; DPH6/MJ0570.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR035959; RutC-like_sf.
DR InterPro; IPR006175; YjgF/YER057c/UK114.
DR NCBIfam; TIGR00290; MJ0570_dom; 1.
DR PANTHER; PTHR12196:SF2; DIPHTHINE--AMMONIA LIGASE; 1.
DR PANTHER; PTHR12196; DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN; 1.
DR Pfam; PF01902; Diphthami_syn_2; 1.
DR Pfam; PF01042; Ribonuc_L-PSP; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF55298; YjgF-like; 2.
PE 4: Predicted;
KW Ligase {ECO:0000313|EMBL:AOW27412.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000559}.
FT DOMAIN 1..235
FT /note="Diphthamide synthase"
FT /evidence="ECO:0000259|Pfam:PF01902"
SQ SEQUENCE 627 AA; 70726 MW; 5A1F84AF2243328B CRC64;
MKFIGLISGG KDSFFNIYHC ISQGHELVAL GNLYPEISDE IDSFMFQTVG HDVIEYYSQC
LDVPLYRQPI LGKSTNQNLE YSVTENDEIE DLYKLLSKIV DAHPEIEGVS CGAILSHYQR
TRVENVCDRL GLTSLTYLWQ RNQYDLMKEM CESGLDARII KVAAIGLTKN HLGKSISELF
PALVKLNLMY EAHICGEGGE FETIVLDCPF FKKKKLVITQ QDIVEHSSDV FYLRIKVELA
DKNVTSDYKP LESLPLLEDE FQDIADSCPK ADISLSGLSI DSQRIVPKID VTPTETTLYI
SNLTSMKSTI EDQTKDIFQQ LDKLLGDHKL TVNDIQHITL LLSDMSNFAK VNKFYGTFFK
DIYLPPSRIC IETEVESIQL SCIVLKHIQP KTGIHIRSRS YWGPQNIGPY SQSIVNTQSS
YKTATLSGQI PLIPATMDIS NKSIAFDSAL SLQHLVRVKN LVNVKQLASV VCFITDESYV
PIVRSCWTDL ENKGNLVIVT VRGLPRGAKV EWGGLSFEDI TGIYDDFASD SEDESDDDSV
SQFDNFDESC ICQVGKKGEF SVVTLFTSDS DVIKSSFTFK GYRQLLCHPK DYIEIKGDYL
PVNTVYDHKG KNHKYAVILK IEVHRKN
//
