ID A0A1D8T9F8_9NOCA Unreviewed; 1215 AA.
AC A0A1D8T9F8;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=BFN03_01930 {ECO:0000313|EMBL:AOW94282.1};
OS Rhodococcus sp. WMMA185.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Rhodococcus.
OX NCBI_TaxID=679318 {ECO:0000313|EMBL:AOW94282.1, ECO:0000313|Proteomes:UP000178767};
RN [1] {ECO:0000313|EMBL:AOW94282.1, ECO:0000313|Proteomes:UP000178767}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WMMA185 {ECO:0000313|EMBL:AOW94282.1,
RC ECO:0000313|Proteomes:UP000178767};
RA Bugni T.S., Adnani N., Braun D., Chevrette M., McDonald B., Rajski S.;
RT "Draft Genome of Rhodococcus sp. WMMA185.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; CP017014; AOW94282.1; -; Genomic_DNA.
DR RefSeq; WP_070380530.1; NZ_CP017014.1.
DR AlphaFoldDB; A0A1D8T9F8; -.
DR STRING; 679318.BFN03_01930; -.
DR KEGG; rhw:BFN03_01930; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000178767; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000178767}.
FT DOMAIN 667..828
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 853..1003
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1215 AA; 131475 MW; D03A372754D53207 CRC64;
MSSPLIATEP SADTPLAGLA KIALGDGVIT QVVEALGRDH LDVVAPPSAR PFVAAALAER
THLLMVTATG READDLTAEL QEMIGDGVAQ FPSWETLPHE RLSPSADTVG RRMEVLRRLA
RPDDPAYGAP LRVIVTTVRS LVQPMAPGLG EIEPITLREG TEHDFDGLIQ RLVDMAYTRV
DMVGKRGEFA VRGGILDFFS PTADHPVRVE FWGDEVTELR AFSVADQRSL PELEIGSVVA
PPCRELLLTE DVRARAAQLA VDNQADAALV EMLDKVSGGI PVEGMEALLP VLRPGQLQLL
TDVLPDGAHI LLCDPEKIRT RATDLVRTGQ EFLEASWTAA SIGGAAPLDT SVLAGGGIDL
GASAYRSLRQ VRESAEAAGL AWWNLSPLAS GSGEELELAV TPAPQVRGSD DLLAELFVNL
RAHVTTGGRA VVVVAGSGTA HRVLERLHEA EVPATELAPG SEPVRGQVGV LRGSLHDGLV
LPGDDATPGL VIVTETDLTG NRVAAVGDGK RLPAKRRNQV DPLALTAGDM VVHDQHGIGR
FVEMVERTIG GARREYLVIE YAASKRGHPG DRLFVPMESL DQLSRYVGGE LPALSKLGGS
DWANTKRKAR KAVREIAGEL VQLYAARQAA PGHAFGPDTP WQKEMEDAFA FTETHDQLTV
ISEVKADMEK AVPMDRVVIG DVGYGKTEIA VRAAFKAVQD GKQVAVLVPT TLLAQQHLQT
FTERMAAFPI TVRGLSRFTH TAEAKEIIDG MADGEVDVVI GTHRLLQTAV RWKDLGLVIV
DEEQRFGVEH KEHIKALRTH VDVLTMSATP IPRTLEMSMA GIREMSTILT PPEERHPILT
YVGAYADKQV AAAIRRELLR DGQVFYVHNR VSSIDKAAQR IRDLVPEARV VVAHGQMNED
TLERTVQGFW ERDYDVLVCT TIVETGLDIS NANTLIVERA DSLGLSQLHQ LRGRVGRSRE
RGYAYFLYPP EIPLTETAYD RLATISQNSD LGAGMAVAMK DLEIRGAGNV LGAEQSGHVA
GVGFDLYVRL VGEAVEAFRA AADGKPIDAT ETPREVRIDL PVDAHIPPNY VTSDRLRLEG
YRKLAAAGVL DEIAAVVEEL VDRYGPLPEE LRRLVSIAKL RLLCREYGIE EVAVVGTQLK
ISPMALPDSK QLRLKRIYPS ARYRATTGMV QLPLPRAGSG GVGADRVRDL ELVQYIADLL
LALDGKAAGS VVMEA
//
