ID A0A1D9NX51_9FIRM Unreviewed; 673 AA.
AC A0A1D9NX51;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=bhn_III010 {ECO:0000313|EMBL:AOZ94958.1};
OS Butyrivibrio hungatei.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Butyrivibrio.
OX NCBI_TaxID=185008 {ECO:0000313|EMBL:AOZ94958.1, ECO:0000313|Proteomes:UP000179284};
RN [1] {ECO:0000313|Proteomes:UP000179284}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MB2003 {ECO:0000313|Proteomes:UP000179284};
RA Palevich N., Kelly W.J., Leahy S.C., Altermann E., Rakonjac J.,
RA Attwood G.T.;
RT "The complete genome sequence of the rumen bacterium Butyrivibrio hungatei
RT MB2003.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR EMBL; CP017830; AOZ94958.1; -; Genomic_DNA.
DR RefSeq; WP_071174790.1; NZ_CP017830.1.
DR AlphaFoldDB; A0A1D9NX51; -.
DR KEGG; bhu:bhn_III010; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000179284; Chromosome ii.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006012; P:galactose metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013739; Beta_galactosidase_C.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08533; Glyco_hydro_42C; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001084-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000179284};
KW Zinc {ECO:0000256|PIRSR:PIRSR001084-3}.
FT DOMAIN 15..388
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 400..604
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT DOMAIN 613..660
FT /note="Beta-galactosidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08533"
FT ACT_SITE 151
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 310
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 112
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 116
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 150
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 156
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 158
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 161
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-3"
FT BINDING 318
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 673 AA; 77722 MW; A0D8196E2BFCEEB3 CRC64;
MVESSKVQKI LYGGDYNPEQ WPEDIWEEDM RLFKEAHIDI VTLNVFSWAQ LQPSEDTYDF
SKLDKIMELV QKNGLKVCMA TSTAAHPAWM ARKYPDILRT EKNGVKRKFG SRHNSCPNSP
TYRKYASKLV EKLAERYKDY DNIVCWHISN EFGGECYCEN CEKRFRSWVK DKYKTIEEVN
RAWNMAFWGH TLYDFEDIVT PDLRSEEFEW DGIRTNFQGI SLDYARFNSD SLLECFLLEK
EILKKYTPDI PVTTNLMGMY KPLDYQKWAE YMDFVSWDNY PTYNAKPYEP ALSHDLMRGC
KNGKPFALME QTPGVTNWHL YCSLKRPGVM RLYSYQAMAH GADTVMFFQM RRSIGACEKY
HSAVIDHVGT SDTRVFREVS ALGKELETIG SSILGARSKA EIAIVFDWDN WRAIDWSAGP
SRLLNYCDQI KKYYYALWRN HYPVDIISVK DDLSKYKLVI APMLYMCKKG YDQTIRSFVE
NGGTFVTTTF SGYVDDNDLV VTGGYPGKLK DILGIWVEEL DALPPEEANS FIYKGTEYPA
QVLCDVLHLR SANELAKYEK DFYAGAPVIT VNAFGAGKAY YFATQSNDDF YKEFLSDICS
ECGVQPLLKC DEDLEVTERS NSNGDFLFVL NHSQEKKEFV LPYDACNLVT GTEYFKGKEI
KISGKDAMIL IKK
//
