ID A0A1E2WNK8_9NOSO Unreviewed; 2418 AA.
AC A0A1E2WNK8;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE SubName: Full=Beta-ketoacyl synthase {ECO:0000313|EMBL:ODG99976.1};
GN ORFNames=A4S05_35430 {ECO:0000313|EMBL:ODG99976.1};
OS Nostoc sp. KVJ20.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=457944 {ECO:0000313|EMBL:ODG99976.1, ECO:0000313|Proteomes:UP000094607};
RN [1] {ECO:0000313|EMBL:ODG99976.1, ECO:0000313|Proteomes:UP000094607}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KVJ20 {ECO:0000313|EMBL:ODG99976.1,
RC ECO:0000313|Proteomes:UP000094607};
RA Wen L., He K., Yang H.;
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ODG99976.1}.
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DR EMBL; LSSA01000184; ODG99976.1; -; Genomic_DNA.
DR RefSeq; WP_069074442.1; NZ_KV757759.1.
DR OrthoDB; 499075at2; -.
DR Proteomes; UP000094607; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd08953; KR_2_SDR_x; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.70.250; Malonyl-CoA ACP transacylase, ACP-binding; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR004432; Omega_3_polyunsat_FA_synth.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR02813; omega_3_PfaA; 1.
DR PANTHER; PTHR43074; OMEGA-3 POLYUNSATURATED FATTY ACID SYNTHASE PFAB-RELATED; 1.
DR PANTHER; PTHR43074:SF1; PKS_AT DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 50..506
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1336..1413
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1468..1545
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 1029..1074
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1430..1470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1551..1581
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 3..30
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1050..1068
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2418 AA; 265121 MW; C9C012C6161541E6 CRC64;
MAASKIEAVL AQLQEEVSNC EQALLKLIQV KKVMVDNAPL TSEIKTHRQK TDIAIIGMAS
IFPQSKNLQE YWETIIHKVD CITDIPASRW NVEDYYDPNP RAFDKTYCKR GGFIPDVNFN
PMEFGLPPNS LEVTDISQLL GLVVAKAAME DAGYGESRQF SRERTGVILG VALGRQLAVP
LTARMQYPVW EKVLKGSGLS DEDTQKIIEK IKSAYVQWDE NAFPGMLANV VTGRIANRLD
LGGTNCVVDA ACASSLGALK MAISELIEHR ADMMITGGVD TDNSIMAYMC FSKTPAVSQG
ENVKPFDAES DGMMLGEGVG MLVLKRLEDA QRDNDRIYAV IKGIGTSSDG RYKSIYAPRP
EGQVRALHRA YEDAGFSPTS VGLIEAHGTG TMAGDPAEFS SIKEVFGENN PKKQHIALGT
VKSQIGHTKA AAGAASLIKT ALALHHKVLP ATINVTKPHP KLDIDSSPFY LNTETRPWIS
SDEQPRRAGV SSFGFGGTNY HVVLEEYTSQ HQQPYRLHKP SQPVLLFAST PEELLSRCQE
IGQQLQSDTG EQLYTELISA SQSLEIPIAN ARLGFVADSL TQASDLLQTS IELLRNKLQA
ESWEHPQGIY YRKTGIATEG KVVALFSGQG SQYLEMGREL LINFPELGQT YAQIDNLLRQ
DGLQPLSEVV FPNPVFDLDQ KTAQMKILQQ TEYAQPAIGA FSAGLYKILQ QAGFKPDFVA
GHSFGELTAL WAAGVLSEKD YFFLVKTRGQ AMAAPEDPEL DAGAMLAVKG DVNQVTELIQ
NFPLITIANL NSPNQMVLAG TKAEIANVEE ALKTQGFSSI LLGVSAAFHT PLVAYAQQPF
ARAIEKVTFN EPQIPVYTNV TGKRYPNDPQ VIQKVLKEHL RNQVLFKQEI ENIYAEGGHC
FIEFGPRSVL TNLVKDILSD RPHLAVALNT SHLKDSDRTL RQAVIQLRVA GLPLQNLDPY
QLEYKIPEAS PNKVLNVRLN STNYVSEKTK QSFEKAMQNG HQVKSTVGNG NQPATANPSH
PAEKVAVNGQ STVGNGNKPA TANPSHPAEK VAVNGQSTFG NGNKPATANP SHPAEKVAVN
GGVKSVQNGQ SDQLDIKSLQ PIPELPLSYL RAIDNLEYTL IEFNRHQHDI LQVHEQSLNH
QTEYTKTFFQ LMQQQHALWG NGKFSEPQTQ TQQLVVSSSE RSIMRFHDHQ ADSIRIHEQY
LNHQQEYTNN FFQLLQRHYE QSPSSTSLIP PINYQPSPVV PPTQKDNSVL ETASVDVPSN
GFATKSEPVS TNGNGTNGNG AHHQAAVLDI NPLEENKTAT VAFVPPAPAE PVIPTEPVAP
AEPVAPPPPA VIIDQAILSE TLINVVSDKT GYPTEMLDLT MDIEADLGID SIKRVEILGA
LLELYPDLPR PNPEEVGQLR TLGQIAEYMG KIASEISAAI PAVGLVNAPV DENGRDGKDG
GDDGDGGVTV TSPSSPSPSS PSSPPSSPEL TNIVSNVVSE KTGYSMTMLD LSMDLETDLG
IDRVKLVEIL GALLELYPDL PKLNSEALAQ LHTLGQIVAY IEKGVGTGDW GLGTGEEFSQ
SPLPNPQSPI PNPQSPVPST QSRILRSPVK LKYLPEPDFL DFTFPDQHIA LLTDDGSLTT
TKLAETLLKR GWKTVVLTFP STVISKQSAL PEGVSRVVLA DLSEEHLQGQ LTAIANNYGA
IAAFIHLNPS NHEDTSKNVR YLESEKAILR HIFLIAKYLK EPLNQAALQG RSSFVTVARL
DGEFGLGHKT NFGAIGAGLF GLTKSLNQEW ESVFCRAIDL NPDLDAEISA QYILAELHDP
NLLVVEVGYN SQGRSTLICE QEEQKNREQQ VGTIPFAPST FSANLFPIAN AQSQVFLVSG
GAKGITAQCA IKLAQRYQCK FILIGRSAAD PEPVWTEGYL SEADLKKRIM EDFLARGEKP
TPAMVQKKFN VISSSREIAT TLQAIKQAGG KAEYLNVDVT DAIALSEQVA NAVKRFGPVT
GIIHGAGNLA DKRIEKKSVQ DFETVYAAKV KGLENLLRCV PPNQLNYLVL FSSVVGFYGN
VGQTDYAIAN EILNKSAHLV KRNHPHSHVV AINWGPWDSG MVSPELKQAF AERNIETIPI
EIGTEFLVDE LASTNQETVQ VVIGSPLVFI PEKLPSELRT FRIQRQLTLA ANPFLQDHVI
AGYPVLPATC GLLWIANACE QIYPGYKAFS CTNYKVLKGL IFDENLPTEY TLELQEIAKH
DINEIDFDAK IWSKTPEGKI RYHFSSQMKL KREVPSTPTL EFLNLSQDHR ITATTSSLYQ
NGAATLFHGL TFQGVKSVLN ATLDKITIEC YLPEPAKRQQ GQFPVQTFNP YIADVQIHAF
WIWAQHFYQV ACLPSEIKIF EQFEAVPFDE TFYVSCEIKS KTESGLVVDV IAHNQQGQIY
TRMLGAKGTI LPKQLDEI
//
