ID A0A1E2WSG1_9NOSO Unreviewed; 611 AA.
AC A0A1E2WSG1;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Protease 4 {ECO:0000256|PIRNR:PIRNR001217};
DE EC=3.4.21.- {ECO:0000256|PIRNR:PIRNR001217};
DE AltName: Full=Endopeptidase IV {ECO:0000256|PIRNR:PIRNR001217};
DE AltName: Full=Protease IV {ECO:0000256|PIRNR:PIRNR001217};
DE AltName: Full=Signal peptide peptidase {ECO:0000256|PIRNR:PIRNR001217};
GN ORFNames=A4S05_30230 {ECO:0000313|EMBL:ODH01149.1};
OS Nostoc sp. KVJ20.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=457944 {ECO:0000313|EMBL:ODH01149.1, ECO:0000313|Proteomes:UP000094607};
RN [1] {ECO:0000313|EMBL:ODH01149.1, ECO:0000313|Proteomes:UP000094607}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KVJ20 {ECO:0000313|EMBL:ODH01149.1,
RC ECO:0000313|Proteomes:UP000094607};
RA Wen L., He K., Yang H.;
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|PIRNR:PIRNR001217}.
CC -!- SIMILARITY: Belongs to the peptidase S49 family.
CC {ECO:0000256|ARBA:ARBA00008683, ECO:0000256|PIRNR:PIRNR001217}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ODH01149.1}.
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DR EMBL; LSSA01000099; ODH01149.1; -; Genomic_DNA.
DR RefSeq; WP_069073467.1; NZ_KV757704.1.
DR AlphaFoldDB; A0A1E2WSG1; -.
DR OrthoDB; 9764363at2; -.
DR Proteomes; UP000094607; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR CDD; cd07018; S49_SppA_67K_type; 1.
DR CDD; cd07023; S49_Sppa_N_C; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR004634; Pept_S49_pIV.
DR InterPro; IPR004635; Pept_S49_SppA.
DR InterPro; IPR002142; Peptidase_S49.
DR InterPro; IPR047217; S49_SppA_67K_type_N.
DR InterPro; IPR047272; S49_SppA_C.
DR NCBIfam; TIGR00705; SppA_67K; 1.
DR NCBIfam; TIGR00706; SppA_dom; 1.
DR PANTHER; PTHR33209; PROTEASE 4; 1.
DR PANTHER; PTHR33209:SF1; SERINE PROTEASE SPPA, CHLOROPLASTIC; 1.
DR Pfam; PF01343; Peptidase_S49; 2.
DR PIRSF; PIRSF001217; Protease_4_SppA; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 2.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|PIRNR:PIRNR001217};
KW Cell membrane {ECO:0000256|PIRNR:PIRNR001217};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR001217};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR001217};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PIRNR:PIRNR001217};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..37
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 133..284
FT /note="Peptidase S49"
FT /evidence="ECO:0000259|Pfam:PF01343"
FT DOMAIN 383..533
FT /note="Peptidase S49"
FT /evidence="ECO:0000259|Pfam:PF01343"
FT ACT_SITE 201
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
FT ACT_SITE 399
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
SQ SEQUENCE 611 AA; 66918 MW; 3BAFD8285855E51C CRC64;
MTNFIKQTFA SLVGTLLGLI IFGGLGATGL FLLILAATSS KDTGPNVKDK SVLVFDLSMK
ITDGEPSSDQ LFQNTISGVD EERITLRKVV ETLEKARRDP RIIGIYLDAT SASQASDVGY
ASLKEIRKAL EEFRAAGKKI VAYGSDWSEK GYYVSSVADS IVLNPLGMME VNGLSSQPIF
LAGALQKYGI GIQVVRVGKF KGAVEPFLLT KLSPENREQT QKLLDDVWGE WRTAVGASRK
IQPNQLQAIA DNQALLEATQ AKTSRLIDQV QYPDEVVTDL KKLTDSDKED KTFRQISLNS
YAQVSGKSLG VERSSKNQIA VVYAEGEIVD GKGEDGQVGG DRFAKIFNRL RQDQDVKAVV
LRINSPGGSA TASEVMQREV KLTREAKPVV VSMGDIAASG GYWIASDSNR IFAEPNTITG
SIGVFGVLFN GQKLANDNGI TWDSVKTARY ADSQTVSRPK SPQELALYQR SVNRIYNMFL
NKVSQGRKLP EQKVAEIAQG RVWSGVAAKE IGLVDEIGGL NTAIAYAAKQ AKLGEDWEVQ
EYPRTSSFGE RFFGRVTEEA QTALGIEGTQ LKQSNPLTTE FQKLQQEVEI LQRMNDPQGV
YARLPFNLKI E
//
