ID A0A1E3L5G9_9BACL Unreviewed; 5777 AA.
AC A0A1E3L5G9;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=Polyketide synthase {ECO:0000313|EMBL:ODP28425.1};
GN ORFNames=PTI45_02175 {ECO:0000313|EMBL:ODP28425.1};
OS Paenibacillus nuruki.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1886670 {ECO:0000313|EMBL:ODP28425.1, ECO:0000313|Proteomes:UP000094578};
RN [1] {ECO:0000313|EMBL:ODP28425.1, ECO:0000313|Proteomes:UP000094578}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TI45-13ar {ECO:0000313|EMBL:ODP28425.1,
RC ECO:0000313|Proteomes:UP000094578};
RA Kim S.-J.;
RT "Genome sequencing of Paenibacillus sp. TI45-13ar, isolated from Korean
RT traditional nuruk.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in some intermediate steps for the synthesis of the
CC antibiotic polyketide bacillaene which is involved in secondary
CC metabolism. {ECO:0000256|ARBA:ARBA00003299}.
CC -!- PATHWAY: Antibiotic biosynthesis; bacillaene biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004789}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ODP28425.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MDER01000038; ODP28425.1; -; Genomic_DNA.
DR RefSeq; WP_069327600.1; NZ_MDER01000038.1.
DR STRING; 1886670.PTI45_02175; -.
DR PATRIC; fig|1886670.3.peg.2215; -.
DR UniPathway; UPA01003; -.
DR Proteomes; UP000094578; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd08953; KR_2_SDR_x; 3.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 1.10.1240.100; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 4.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 3.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR013149; ADH-like_C.
DR InterPro; IPR013154; ADH-like_N.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF00107; ADH_zinc_N; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF08242; Methyltransf_12; 2.
DR Pfam; PF21089; PKS_DH_N; 3.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 3.
DR SMART; SM00826; PKS_DH; 3.
DR SMART; SM00829; PKS_ER; 1.
DR SMART; SM00822; PKS_KR; 3.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF50129; GroES-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 4.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000094578};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 404..478
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 899..1314
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2273..2350
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2419..2853
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4376..4814
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT REGION 2369..2421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 5777 AA; 648294 MW; 278F654746E7B6EC CRC64;
MKNFKMMESA NQQMEAVNEL LIQLLWCQLH ATGCLNKDFV WQKGTLSFYP QWLVESIAML
QAQVQLLPTH KDNLKDTNIY KQVVDRYWDK WEKQLSGWLQ NPDLQAQVQL VDRMLRNLPS
ILQGDRLATE IMFPESSMQL VEGIYKHHST ADYFNTVLAE SASAYVEKCA QAGRKVRILE
IGAGTGGTSE VVFEHLKIWE SHIEEYVYSD ISKAFLMHAE QKYGPSVPYL NYQIIDLKKS
FASQGVDLGA YDLIIASNVL HTTQNIRHTV QTVKTTLKKN GMLLVNEITD KSLINHLTFG
LLEGWWAYED KALRISGSPA LSADSWKKIL KREGFHTITY PASEAVMFKQ QIIASQSDGI
VIHARNNIIP EVDIPIIPPN HTFMRKQAKE DASVNAVTDQ AVLDFVSDIV RGSIAEDLKI
NTDRIQNDRS FAEYGVDSII AVHLVNLINK RGQLSLQTTV LFDYNNVDLL VEYLLEEYSA
HFKQMLQVAK DSSEASEPVS TIQDNLTPSK VSVSVQPSKE DTKYNSSPIN RIISEEHTIT
PNASDTSFYQ VVLHRPSDIQ ELSIQAHEVP ALKEDEVRIA VRAFSLNFGD LLSVKGMYPT
MPPYPFTPGF EAAGIVVDVG AQVTHIRSGE DVVFFASEHM GAHATAITCK ASQVFAKPST
LSFAEACSLP VVAMTVIAAF GKVQIRPKQR ILIQTATGGV GLVAIQLAKH YGAEIYATAG
SQHKLDYLLS IGVDHVINYR EVDFEKQIKQ LTNGQGVDII LNTLSGDALQ KGLNSLASNG
SYIEIAMTAL KSAKAIDLSQ MGSNQSFYSL DLRKLSLEQP ERLVEYYNEM LDWVAKGIIV
PHIGQLFRFD QLQEAYRNIE NRENIGKIVV TIPEEWQYHV VPQSKPKPAL SSNQDIHSRE
PIAIIGISGR FAGSNTPDEL WKHLEQGTDL IQRVSRWDLS QYYDADQEYC DQGSFLDDIS
TFDPLFFNIS GQEAIYMDPQ QRIFLEESWK ALEDAGYAGH SIKGQKCGVY VGCTSSDYPT
LVGDNPPPQS FWGKAGSIIP ARIAYYLDLQ GPAIAVDTAC SSSLVAIHLA CQALWANEIN
MALAGGVFLQ STPQFYLDSN RAGMLSLEGR CHTFDEKADG FVPGEGAGTI VLKRLHEALE
DGDHIYGVIQ GSGINQDGTT NGITAPSAKS QTRLEQQVYD SFNINPAHIQ MVEAHGTGTK
LGDPIEYQAL TKAFSTYTNE QEYCAIGSIK TNIGHLATAA GIAGILKIVL SLKNKKIPAS
LHLEKGNANI SFSDSPFYVN TTTKEWEVPI DQARRAAISS FGFSGTNAHM VIEEAPQTGA
QSQVSKAGYL IVLSARTEQQ LRQQAQQLLS WNMNHTADCA AISYTLLSGR QHLNHRLACI
VQSQQELTTY LQQWLDQGES PEVTVGQVNR SENREQLSFK KYAADCIQKC QQAKQDASYL
EYLVAVKDVF VQGYEWNLGD IFAKEEQHRI PLPTYPFAKN QYWVPALSTP AARFTSSKSV
AVESIHPLLH KNTSDLQQQK YTSIFTGNEF FFRDHIVQGN KVLPGVAHLE WIRAAVTEAI
GGIPDHQIIQ LKNIVWTSPL HLEIDPFIAH IDIYVESNGD LSFEIYSDST ENRDRLVYSQ
GSASIIEQAP PTAIDIVHLQ HQYSISPISA NQFYEQSQKN GITLGESLKG IEVVYTKATE
DHEVLARLKM PVDQLPDKEL YQLHPSMMDS ALQVAIGYTI GTVSNPLYQG KSTLPFTLQK
LEIFQPCLPE MWVWARYSAG STANDKIHKL DIDLCDNEGH ICVRFSGFTP RTLEHAITAS
SDHHDSMLLT PTWTEQTVLS HSDHTNRNIH HIIVLAPSFA SIYNDIRTSM PHVHCIILQE
EEIAYVTTEE QNAHLFTSCT VQLIDVLKQS ISNSSENILL QLIVPNKGKY HTLLGLAATL
QTAQQEYSKF KTQCIQVGLQ ETAINVLHKI KDNQNESVSI VRYDNQIRYI RDWNKWEPTL
SEESQLPWKD QGHYLITGGL GGLGYIFAQE IIQQTNQVTL IIVGRSPLDH FMQAKLDQLQ
HHQAQIHYLV ADITIAEDVT TMIDQIRSIC GELHGIIHSA GIHRDHYIQH KVNEEIEEVL
APKVHGTLLL DQVSADMPLD FFVLFSSLAS VVGNTGQADY ATANAFMDIF ASERNHKVTY
QQRSGKTLSL NFPLWEDGGM QLNTVERTRI RKQLGMIPMD ADTGIQLFYK AIKSSQDQVV
VLHGDTDKLQ SLLQPFVAES LTEQHASTIL HHDSTPSILD PFIEGKSEMN STPLIEKMQS
ALIQIVSHML KVSSKDIYPD VELNEYGFDS ISFTDFSNQL NEKYHLDLTP TVFFEYSTLH
TLAKHLVTTY AVQLTSTLMP NVQRKVPFTS DSTKTVDNGL KQEAESVNSR SIPTTRSRFQ
KPVETETSYQ PVSSTDQSPD PVAIIGMSGR FPMAEDIHAL WENLIEGKDC ISEIPADRWD
WKAYYGDPYQ EKNKTNIKWG SFIDGVADFD PMFFGISPRE AELMDPQQRL MMMYIWKAIE
DAGYAPSSLS GSKMGIFVGT ANSGYSHLLS QANTEIEGYS STGSVSSVGP NRMSFFLNVN
GPSEPIETAC SSSLVALHRA VQAIHNGDCD TAVVGGVNTI LDPDYHISFN KAGMLSQDGR
CKTFSDQVNG YVRGEGVGMI FIKRLKDAEQ AGDHIYGLIR GTSENHGGRA TSLTAPNPRA
QADLIKSVYS RAHVDPATIS YIEAHGTGTP LGDPIEINGL KMAFNELGYD AASNTATCGL
GSIKTNIGHL ELAAGIAGII KVLLQMKHQT LVKTVHFEQL NPYIQLEQSP FYIVDTNRSW
VPLQDEQGNV LPRRAGVSSF GFGGVNTHVL VEEYVPSSLN TDSSAHEESV LIVLSARNEQ
RLREQAKQLR IAMQSDRYTD HELERIAYTL QVGREAMEER LAIRVSSIHE LITQLNNYIE
QTDSTLWNDH MYVGQVKRDQ EVLEFLTADE DMQETVQRWM QKGKYHKLLK LWVKGMDIDW
ATMYIDRSLQ RISLPTYAFE NKRYWIPNEL TIDSNQNTTA NESNMIHPLL HHNTSTLIQQ
RFTSTFTGKE FFLDDHVIKG QRILPGVAYL EMARASVVQA LELYEHQESM IVFKNVTWVR
PIAVDKEPVQ IHIQLIPQND QVLHYEIYSQ QDDALTTLYN QGTVTVVPTT KEHKEIDISS
LIQKCDQQMI IGEECYASFK QVGLYYGPAQ QALQNIYVGH QQAIAQLSLP PTVSHTEEQY
ILHPSMMDAA IQACIGLPSL GYDSVTPFAL DEMRIYQSCT STMWAWVRYQ DTPEQQKSLD
KVDIDICNEQ GELCIELKGL SILHLSHHQD DVQHTVSAPP VGALMQYPVW DSVIPEFTAS
HILPSTHVLC IGETPALWLN LIQDYPQIRY AQLEIDADVD QITALLEKEP VIDHIVWSIS
NTIHLDLLHV PVHQPSKGII QIFRILKSLL QLGYGEKDLA WTIVTAYTQP LSVNENVYAE
HAGIAGLVGS LAKEYSNWNI QHVDMEDHFD IATCDLLTLP ADLEGNTWLY REKQWYRQQL
MIVDAPKESV TSFQQNGVYV VIGGAGGIGE VWSQYMINTY NARIVWIGRR PLNPQIQNKI
DLLGQQGIAP LYISADASNL QSLTEAYQTI KQSYSSINGM IHSAVGVMDA SLSHMQEEHF
QASLSAKIDI SLAMMQVFAK EPLEIVLFFS SMSSFSKPIG QSSYAAGCTF TDTFAYEMRK
QFEFPIKVIS WGYWGEIGIA EKVPTAFKQR LIQSGVGFIE PKEAMQAIEY LVGNAIDHMT
FIRTTQPDAV ITTHADEQLW IAPEVNAIAI ETLQRDYKDH AKVNSLMQTM QIFDQELETL
LAHLLYYQLQ QAQCLEHHNE VIEVYERWLD HSRLMLKQKN IDDLYGSTEQ LWIQWEHRKE
VWIQHPDLRA QVVLVDTMLK HLPDIIKGKV LATDIMFPDA SMSLLENLYK NNAVADYFNE
KLADTVVSYI QARLQHDPSA SIRILEAGAG TGGTSSIVLK KLKPYTDAIA EYAYTDLSKA
FLNRAEMMFG QDHSYLKYQL FNVEQPVQEQ SLSIGSYDIV IAANVLHATQ NINRTAKHIH
SLLKRNGLLL LNELNRSTLF SHLTFGLLEG WWMYEDASIR ILGSPVLDEH HWRTALEYTG
FHTVTFPTKS AQPLGQQIIV AASQGLIRLP RSQSNNLLLN NMDKKTMEQA RTINTTSMEQ
DISHPHEHQE NVQINLNLAT EEPITEEQML QHSKPYFQQL IGDLVKLPYD QIDISEPLEK
YGIDSILIVQ LTNKLRKHFD SINSTLFFEH QSLLALMKYF IKEHQQQLIE LLEINPSQST
RVASTSLLTN NSSKVISDKG YRKLRMPVQE MQAQEPTVPP VAQSVSSAHS TSPIQQEPIA
IIGMSGRYPQ ADNLEQYWYN LEHGIDCISE IPEERWSIEG FYNPDRKHAV AQGQSYSKWG
GFINGFADFD PLFFKLSPRE AINMDPQERI FLQSVWESLE DAGHTREKLT EHYKSRVGVF
AGITKTGYNL YGPELWKQGS DLFPMTSFSS VANRVSYLLD LHGPSKPVDT MCSSSLTAIH
EACESIRSGA CELAIAGGVN LYLHPSNYIG LCAQQMLAAD SQCKSFAEQA DGFVPGEGVG
VLLLKPLSKA IADGDAIHAI IRGTGINHGG KTNGYMVPNP TAQANLIRDT MDRAGIHAQE
ISYIEAHGTG TALGDPIEVS GLTKAFRIDT QEQQYCAIGS VKSNMGHLES AAGIAGITKI
ILQMKHKKIA PTLHATRLNP EIPFEQTPFA VQQHLSDWKR PQINIEGQLK EVPRIAGISS
FGAGGSNAHV ILEEYIAQPT ELQSQSEPID LQPTMIVLSA RQSEQLIERA RQLLSFLEHA
VSSHSSLTDI AYTLQVGREA MEERVAWMVH SVSDLQSKLT SFIQQPDEHM PNYYKGTVKR
LFDMTSMFDS EEHMQQIIND YYLQGQYDAL LELWIQGVPI HWSALNRSVT SNIVHLPAYP
FVKEKYWLAP MQTETDIAIS DQDRERNAST VIDKMVLHPL LHRNTSQLSE QRFTSIFNGQ
EFFFADHVVN GNNILPGVAY LEMVREAIAQ STSTSLDRTL WSLENIIWAT PIRMEDRELA
VHVALYAEDE NHIQYEVYTE QESHHVTTRT IHNQGRAVQQ SLTDAPILDL ATIQKSVKEE
IIATTCYEKF EQMGLYYGER LQSISSLQIG TMGVLAPLNI PKMLEASLSS YVLHPSMMDG
ALQASIGLIV GSHHSADRSN APLLPFALEQ IRIFSACSAS MWSYIRYSEG SDPMDKVQKL
DIDICHENGE VCASLKGFTS KRLESLQAPL YPKETIMLVP EWLEQDTVTV PEISFHQHII
MVCSPATHIA EQIRRELPDA LCIQLGCDGN QPLEQRFGTA ALEAFTQVQR LLQSKPEGSI
LVQLVVVHDQ DYQLYTALSG MLKTAELEKP KLIVQMIELD GLAAAFDPVD KLCECRDSAG
DTHISYHNYR RYIATWQEFV PSATSIKQAW KDQHVYLITG GMGGIGYLIA QDIAQSVTEP
ILIITGRSML DKEIQTKIDK LQEYGAQAFY YVSDITNREE VSTLMNTIQK EFGRLNGIIH
SAGVLKDSFM IRKTPEEWNN VLEAKVNGTI YLDQYSQLFE LDFFVLFSSL AGVLGNIGQS
DYATANAFMD QYAHYRNKLV HTAKRTGHTL SINWPLWEQG GMQMDTQRST SMKEISGMLP
IDQSTAIDAL HQALQHEQSQ IVVMYGNGVQ IRSFMDVDKG RDLDVQDQYF LEMFDHIQSG
QYSLDQLLEQ HNQSKPF
//
