ID A0A1E3L9W1_9BACL Unreviewed; 931 AA.
AC A0A1E3L9W1;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=PTI45_00214 {ECO:0000313|EMBL:ODP30493.1};
OS Paenibacillus nuruki.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1886670 {ECO:0000313|EMBL:ODP30493.1, ECO:0000313|Proteomes:UP000094578};
RN [1] {ECO:0000313|EMBL:ODP30493.1, ECO:0000313|Proteomes:UP000094578}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TI45-13ar {ECO:0000313|EMBL:ODP30493.1,
RC ECO:0000313|Proteomes:UP000094578};
RA Kim S.-J.;
RT "Genome sequencing of Paenibacillus sp. TI45-13ar, isolated from Korean
RT traditional nuruk.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ODP30493.1}.
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DR EMBL; MDER01000002; ODP30493.1; -; Genomic_DNA.
DR RefSeq; WP_069325698.1; NZ_OY725677.1.
DR AlphaFoldDB; A0A1E3L9W1; -.
DR STRING; 1886670.PTI45_00214; -.
DR PATRIC; fig|1886670.3.peg.215; -.
DR Proteomes; UP000094578; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Pyruvate {ECO:0000313|EMBL:ODP30493.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000094578}.
FT ACT_SITE 153
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 587
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 931 AA; 107095 MW; 6E4A5934CD23F3D6 CRC64;
MSELTVNASK NHSNNLLRRD VRFLGNILGE VLVHQGGQEL LDIVEKIREL SKSLRAVALP
EVFEEFKTLI KNLDSDNRHQ VVRAFAIYFQ LVNIAEQNHR IRRKRDYERS AGETIQPGSM
ESAVQELKEQ NFTADEVEAI LADLSLELVM TAHPTEAMRR AILDIHKRIS EDVTLLDNPT
LTFREREQLR EKLLNEVITL WQTDELRDRK PTVLDEVRNG MYYFHETLFD VLPELYQELE
RSLSKYYPEH EWHVPSFLRF GSWIGGDRDG NPSVTADVTW KTLQMQRRLA IREYQRILTD
LMKSLSFSTT IIDVSEELLS SIQKDRLHVT IDKKFSWNNE NEPYRIKIAY MLRKLNNILD
ESKKGTAESY SHMGELLEDL RVIDQSLRHH YADYVANTYV KKLIRQVELF GFHTATLDIR
QHSQEHENAL TEILASMKIV EDYSKLSEEE KIELLGKLLT DPRPLTSTYL NYSESTEECL
AVYRTIFKAQ KEFGTNCISS YLISMTQGAS DILEVMIFAK EVGLFRQDPD GTVHSTLQAV
PLFETIDDLH AAPQIMRQVL NLPAYRQSVA AMNNLQEIML GYSDSNKDGG VVTANWELRL
ALNEITAMGA EYDIKLKFFH GRGGALGRGG MSLNRSILAQ PPHTVGGGIK ITEQGEVISS
RYSLQGIAYR SLEQATSALI TSAIIAKVGE PEGENNKKWE QIMEDISEVS LHKYQDLIFR
DESFMSFFKG STPLPEVGEL NIGSRPSKRK NSDRFEDLRA IPWVFAWTQS RYLLPAWYAA
GTGIQHFYQD KEENMAVLQE MYKEFPFFTT LVDTLQMAIA KADLIIAKEY AGMYKDEEQR
ERIFGLIQEE FNRTRDLVLK ITHQQDILDN VPVIQESVRL RNPYVDPLSY LQVQLLNELR
ELREQDEDNP ELLREVLLTI NGIAAGLRNT G
//
