ID A0A1E3LAN7_9BACL Unreviewed; 843 AA.
AC A0A1E3LAN7;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Arabinan endo-1,5-alpha-L-arabinosidase {ECO:0000313|EMBL:ODP30235.1};
DE EC=3.2.1.99 {ECO:0000313|EMBL:ODP30235.1};
GN ORFNames=PTI45_00305 {ECO:0000313|EMBL:ODP30235.1};
OS Paenibacillus nuruki.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1886670 {ECO:0000313|EMBL:ODP30235.1, ECO:0000313|Proteomes:UP000094578};
RN [1] {ECO:0000313|EMBL:ODP30235.1, ECO:0000313|Proteomes:UP000094578}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TI45-13ar {ECO:0000313|EMBL:ODP30235.1,
RC ECO:0000313|Proteomes:UP000094578};
RA Kim S.-J.;
RT "Genome sequencing of Paenibacillus sp. TI45-13ar, isolated from Korean
RT traditional nuruk.";
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ODP30235.1}.
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DR EMBL; MDER01000022; ODP30235.1; -; Genomic_DNA.
DR RefSeq; WP_069325799.1; NZ_MDER01000022.1.
DR AlphaFoldDB; A0A1E3LAN7; -.
DR STRING; 1886670.PTI45_00305; -.
DR PATRIC; fig|1886670.3.peg.328; -.
DR Proteomes; UP000094578; Unassembled WGS sequence.
DR GO; GO:0046558; F:arabinan endo-1,5-alpha-L-arabinosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18832; GH43_GsAbnA-like; 1.
DR Gene3D; 2.40.128.10; -; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR046780; aBig_2.
DR InterPro; IPR032291; Abn2_C.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR006558; LamG-like.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF20578; aBig_2; 1.
DR Pfam; PF16369; GH43_C; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR Pfam; PF13385; Laminin_G_3; 1.
DR SMART; SM00560; LamGL; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:ODP30235.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ODP30235.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000094578};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..843
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5038551610"
FT DOMAIN 705..835
FT /note="LamG-like jellyroll fold"
FT /evidence="ECO:0000259|SMART:SM00560"
FT ACT_SITE 53
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 302
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 236
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 843 AA; 91756 MW; BED6B873ACBE4CB3 CRC64;
MNKSWIQKAT ALTLMISLTI PAIGFGGATS HAEEYELKQA VTSPTFSNVS VHDPSIIKAG
GTFYVFGSHI SAAKSQDLMN WTSFANGYTT PGNTIYGDLS KNLAGSFKWA GENDADSKGG
FSVWAPDVIW NPKYVNADGT KGAYTIYYCT SSTYIRSAIG MAVSQKIEGP YTYVDTMIYS
GFTKDKAFDN NSTVDKKWTN TNIQTLINQK KLSGESSAWF NADGTYANQN YPNAIDPTIF
SDTKGKLWMT YGSWSGGIFV LEIDAKTGKP IYPGKDGQTK DGRLIDRYFG TKVAGGYGQS
GEGPYIQYNK ESGYYYLYMT YGGLAANGGY NMRVFRATKP DGPYQDAKGQ QATWPAQTSN
VTYGNKLIGN YLWDSKVGDP GYGENFGYVS PGHNSVYTDD KTGQTFLVFH TRFPARGEEH
ELRIHQMYIN KEGWPVVSPY RYAGETLAKV SEEMVVGDYQ FIDHGNDSSA AIKNTQYIRL
NKDRTISGEL QGTWQKKGTA DAILTIDGVK YTGVFVKDWN PVTKQYNMTF TALSTNGTMG
WGSRLVEESD KQVVADVYSD LSLGDVSNVI SNVQLPTVAS RGTSVVWTSS NPAVVSNTGV
VTRPQAGQAP ANVTLTASIS KGNIKQTKVF QVTVQPFVEA TLTAQYSFEH NLKDTTATFG
QGTVTGDRLD STVGTITYGT GVSGQSAVFN GTSGIRLPQG LISNNSYSVA VWVKPDQLTT
YSPAFFGAKD TDHWVSLIPR GATADKAMVW SGTAWYDGVT GVTIPANQWT HLAFSVDEGS
LTIYVNGVQQ FSGTNFPDIF TGGNASFGLG VNYWDTPFKG QMDELRIYKG SLTPAQLSQL
AKS
//
