ID   A0A1E3NUN9_WICAA        Unreviewed;       975 AA.
AC   A0A1E3NUN9;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   RecName: Full=NFACT RNA-binding domain-containing protein {ECO:0008006|Google:ProtNLM};
GN   ORFNames=WICANDRAFT_65727 {ECO:0000313|EMBL:ODQ56846.1};
OS   Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL
OS   Y-366-8) (Yeast) (Hansenula anomala).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX   NCBI_TaxID=683960 {ECO:0000313|EMBL:ODQ56846.1, ECO:0000313|Proteomes:UP000094112};
RN   [1] {ECO:0000313|EMBL:ODQ56846.1, ECO:0000313|Proteomes:UP000094112}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8
RC   {ECO:0000313|Proteomes:UP000094112};
RX   PubMed=27535936; DOI=10.1073/pnas.1603941113;
RA   Riley R., Haridas S., Wolfe K.H., Lopes M.R., Hittinger C.T., Goeker M.,
RA   Salamov A.A., Wisecaver J.H., Long T.M., Calvey C.H., Aerts A.L.,
RA   Barry K.W., Choi C., Clum A., Coughlan A.Y., Deshpande S., Douglass A.P.,
RA   Hanson S.J., Klenk H.-P., LaButti K.M., Lapidus A., Lindquist E.A.,
RA   Lipzen A.M., Meier-Kolthoff J.P., Ohm R.A., Otillar R.P., Pangilinan J.L.,
RA   Peng Y., Rokas A., Rosa C.A., Scheuner C., Sibirny A.A., Slot J.C.,
RA   Stielow J.B., Sun H., Kurtzman C.P., Blackwell M., Grigoriev I.V.,
RA   Jeffries T.W.;
RT   "Comparative genomics of biotechnologically important yeasts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:9882-9887(2016).
CC   -!- SIMILARITY: Belongs to the NEMF family.
CC       {ECO:0000256|ARBA:ARBA00008318}.
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DR   EMBL; KV454215; ODQ56846.1; -; Genomic_DNA.
DR   RefSeq; XP_019036053.1; XM_019183976.1.
DR   AlphaFoldDB; A0A1E3NUN9; -.
DR   STRING; 683960.A0A1E3NUN9; -.
DR   GeneID; 30201222; -.
DR   OrthoDB; 129892at2759; -.
DR   Proteomes; UP000094112; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProt.
DR   Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR   InterPro; IPR021846; NFACT-C.
DR   InterPro; IPR008532; NFACT_RNA-bd.
DR   PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR   PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR   Pfam; PF11923; NFACT-C; 1.
DR   Pfam; PF05670; NFACT-R_1; 1.
DR   Pfam; PF05833; NFACT_N; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Reference proteome {ECO:0000313|Proteomes:UP000094112}.
FT   DOMAIN          547..655
FT                   /note="NFACT RNA-binding"
FT                   /evidence="ECO:0000259|Pfam:PF05670"
FT   DOMAIN          851..951
FT                   /note="NFACT protein C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF11923"
FT   REGION          438..469
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          703..795
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          331..365
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COILED          500..534
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COILED          798..829
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COMPBIAS        438..456
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        703..721
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        722..753
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   975 AA;  112337 MW;  50BA2639ABECE481 CRC64;
     MKQRVTSLDL QILVSELQNE ITNYRLQNIY NIATSNRQYL LKFGLPDSKK NLVLDCGFKL
     HLTEFSRPTP QAPSNFVVKL RKHLKSRRLS SIKQVGIDRV LVLSFSDGMY HLVLEFFSAG
     NVVLLDHERR IMALQRLVEE KGNNDRYAVG ETYNLFDESL FEEDEQVEPK FVPIQHDAST
     IQQWINDKKV TPSDDKGKKN KVLSIQKVLY QKVPYLSADL ISKNLHKHSI NPSSSSLEYL
     ERADELDTVL KETESEVQTI LHTKPLTGYL VLKHNPLYDA SKDEEDTKFL YEQFHPFEPA
     QLKDDEELVQ IQGYNKTLDK FFSTIESSKY ALRIQNQENQ AKKRLDQARN DKQQQVQRLV
     DVQAVNSLKG ETIIFNADIV EEAKAAVQAL LDQSMDWKTI EKLIKIEKGK NNKVASAINL
     PLNLKENKIS LNLLTEAAYE DDEEEESESS SDSDSDSDED EKPKAKKDNK VKNSINVTIS
     LGLSAFANAS EYFTVKKSTV EKQKKVEQSA SKALKNIEQR IEKDLKKNLK QENDILRKLR
     NPYFFEKFNW FISNENYLII SGKDDSQTDL IYHRYVNDDD IYVNADIDGS SHVFIKNPNK
     GEVSPSTLMQ AGILSLATSK AWDNKMVTSP WWLYASDVTK NDIDGTILKA GSFRYLKEKN
     FLPPSQLVMG FAFLWKIKTE ENQKELEQQL EELDNLDIND IQQDDKESNE VESKAPEPVE
     EVKEEEPETT EDAEEAEEGE DQESDDQEET PELESQFDTM SLASKASKQK KPVRGKKSKM
     KKIASKYADQ DEEERRLRME ALGTLKQQNQ REEELKKQQI QKINHLKKVE KKKRQEEITA
     NKYANYKENI NYDKIMNELT PTLHKEDEIL EAIPVFAPWS ALQKYRYRVK IQPGNTKKGK
     AVQEVLHYLN MRPTDPSESD RNYDWPREHE AIAGLKDTEL LPSIYVGKLK VLLPGKSSAA
     KSTNKFANSR KSKKK
//