ID A0A1E4SKR5_9ASCO Unreviewed; 943 AA.
AC A0A1E4SKR5;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Tetrahydrofolate synthase {ECO:0000313|EMBL:ODV80096.1};
GN ORFNames=CANTADRAFT_25819 {ECO:0000313|EMBL:ODV80096.1};
OS Suhomyces tanzawaensis NRRL Y-17324.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Suhomyces.
OX NCBI_TaxID=984487 {ECO:0000313|EMBL:ODV80096.1, ECO:0000313|Proteomes:UP000094285};
RN [1] {ECO:0000313|Proteomes:UP000094285}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL Y-17324 {ECO:0000313|Proteomes:UP000094285};
RG DOE Joint Genome Institute;
RA Riley R., Haridas S., Wolfe K.H., Lopes M.R., Hittinger C.T., Goker M.,
RA Salamov A., Wisecaver J., Long T.M., Aerts A.L., Barry K., Choi C.,
RA Clum A., Coughlan A.Y., Deshpande S., Douglass A.P., Hanson S.J.,
RA Klenk H.-P., Labutti K., Lapidus A., Lindquist E., Lipzen A.,
RA Meier-Kolthoff J.P., Ohm R.A., Otillar R.P., Pangilinan J., Peng Y.,
RA Rokas A., Rosa C.A., Scheuner C., Sibirny A.A., Slot J.C., Stielow J.B.,
RA Sun H., Kurtzman C.P., Blackwell M., Grigoriev I.V., Jeffries T.W.;
RT "Comparative genomics of biotechnologically important yeasts.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the
CC formate--tetrahydrofolate ligase family.
CC {ECO:0000256|ARBA:ARBA00006985}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the tetrahydrofolate
CC dehydrogenase/cyclohydrolase family. {ECO:0000256|ARBA:ARBA00005559}.
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DR EMBL; KV453911; ODV80096.1; -; Genomic_DNA.
DR RefSeq; XP_020065218.1; XM_020207544.1.
DR AlphaFoldDB; A0A1E4SKR5; -.
DR STRING; 984487.A0A1E4SKR5; -.
DR GeneID; 30981681; -.
DR OrthoDB; 651667at2759; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000094285; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR CDD; cd01080; NAD_bind_m-THF_DH_Cyclohyd; 1.
DR Gene3D; 1.10.8.770; -; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.10860; Leucine Dehydrogenase, chain A, domain 1; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_01543; FTHFS; 1.
DR HAMAP; MF_01576; THF_DHG_CYH; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000672; THF_DH/CycHdrlase.
DR InterPro; IPR020630; THF_DH/CycHdrlase_cat_dom.
DR InterPro; IPR020867; THF_DH/CycHdrlase_CS.
DR InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom.
DR PANTHER; PTHR48099:SF5; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR48099; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED; 1.
DR Pfam; PF01268; FTHFS; 1.
DR Pfam; PF00763; THF_DHG_CYH; 1.
DR Pfam; PF02882; THF_DHG_CYH_C; 1.
DR PRINTS; PR00085; THFDHDRGNASE.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
DR PROSITE; PS00766; THF_DHG_CYH_1; 1.
DR PROSITE; PS00767; THF_DHG_CYH_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000094285}.
FT DOMAIN 3..119
FT /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT catalytic"
FT /evidence="ECO:0000259|Pfam:PF00763"
FT DOMAIN 123..290
FT /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF02882"
SQ SEQUENCE 943 AA; 100785 MW; 6BD4631941E7BD85 CRC64;
MIIDGKAIAL DVRTKIHEEI RAIQAKSADF KPSLTIIQVG ARADSTTYVK MKLKAAEEAA
IACNIINLPE SATEFELLNH VQKLNESVDV DGILVQLPLP AHIDEAKVTS AVLPDKDVDG
FGPFNVGELS KKNGEPTFLP CTPKGIMELL ARYNVQIDGA NAVVLGRSDI VGKPVASLLT
KANATVTTLH SKTPQAQLEL FLSQADIVVA AIGQPAFVKG EWLKEGSVVI DVGTNFVDDA
TKKSGSRMVG DIDFESASTK AALITPVPGG VGPMTVASLL ENVVIAAKKH HVKNNATPKF
TNPLKLNLVK PVPSDFEISR AQQPKKIDQV AYESGILDSE LELFGSYKAK VGLHLLERLK
NKKNGKYVLV TGITPTPLGE GKSTTTVGLA QALGAHLNKN VFANVRQPSM GPTFGIKGGA
AGGGYSQVIP MDEFNMHVTG DIHAITMANN LLAAAIDTRM FHESTQKDGP LYKRLVPAKK
GTRSFSPSML RRLAKLGINK TDPDSLTPEE ITQFARLDID PETITWRRVV DCNDRFLRGI
TVGEAATEKG FTRKTGFDIT VASECMAILA LANSLEDMRE RLGNMVIASS KAGVPITAED
IGCAGALTAL LKDAIKPNIM QTLEGTPVFV HAGPFANISI GASSILADKM ALKLAGTSPD
LSEEERKEQE GYVVTEAGFD FTMGGERFIN IKCRSSGLVP DVIVIVATVR ALKVHGGGAE
VKAGAPLAAE YTQENVEFLR AGCANLAKHI SNAKSYGLPV VVAINKMSSD TDREHDVIRE
EALKAGAADA IVSNHWEEGG LGAVDLARGV IEAANSDDKH FKFLYDTAPS VEEKIATIAR
EMYGAGEVEF SPEAQKKIDT YTKQGFGNLP ICIAKTQYSL SHDAALKGVP TGFKFPIRDV
RASIGAGYLY ALAAEIQTIP GLPTHCGFMN VEVNKDGEID GLF
//
