ID A0A1E4U1C1_PACTA Unreviewed; 919 AA.
AC A0A1E4U1C1;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=leucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013164};
DE EC=6.1.1.4 {ECO:0000256|ARBA:ARBA00013164};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030520};
GN ORFNames=PACTADRAFT_83214 {ECO:0000313|EMBL:ODV97801.1};
OS Pachysolen tannophilus NRRL Y-2460.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetales incertae sedis; Pachysolen.
OX NCBI_TaxID=669874 {ECO:0000313|EMBL:ODV97801.1, ECO:0000313|Proteomes:UP000094236};
RN [1] {ECO:0000313|Proteomes:UP000094236}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL Y-2460 {ECO:0000313|Proteomes:UP000094236};
RG DOE Joint Genome Institute;
RA Riley R., Haridas S., Wolfe K.H., Lopes M.R., Hittinger C.T., Goker M.,
RA Salamov A., Wisecaver J., Long T.M., Aerts A.L., Barry K., Choi C.,
RA Clum A., Coughlan A.Y., Deshpande S., Douglass A.P., Hanson S.J.,
RA Klenk H.-P., Labutti K., Lapidus A., Lindquist E., Lipzen A.,
RA Meier-Kolthoff J.P., Ohm R.A., Otillar R.P., Pangilinan J., Peng Y.,
RA Rokas A., Rosa C.A., Scheuner C., Sibirny A.A., Slot J.C., Stielow J.B.,
RA Sun H., Kurtzman C.P., Blackwell M., Grigoriev I.V., Jeffries T.W.;
RT "Comparative genomics of biotechnologically important yeasts.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001372};
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DR EMBL; KV454011; ODV97801.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1E4U1C1; -.
DR STRING; 669874.A0A1E4U1C1; -.
DR OrthoDB; 5472610at2759; -.
DR Proteomes; UP000094236; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07959; Anticodon_Ia_Leu_AEc; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00395; leuS_arch; 1.
DR PANTHER; PTHR45794:SF1; LEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR45794; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000094236}.
FT DOMAIN 21..594
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 635..756
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 919 AA; 105128 MW; D45BC1BFAB85B366 CRC64;
MMQLGIPLEE ISKFADPTYW LYYFPQLCEK DVTSFGGRVD WRRSMITTDV NPYYDAFVRW
QMNRMKELGM IKFGERYTIY SVKDGQPCMD HDRQSGEAVL PQEYTAIKIE VVEFAPSAKE
FFNENKFDTA GKKLYLVAAT LRPETMLGQT CCFVSPNIDY GIFDAGNNEY YICTERAFKN
MSYQKLTPKR GYFKSVIDIN GKTLIGSKIH APLAVHKELR ILPMETVLAS KGTGVVTCVP
SDSPDDFITQ KELFHKPEYY GIEKEWAAPE LVPIIKTEKY GEKCAEFLVN ELKIQSPKDS
IQLAKAKELA YKEGFYNGIM IAGKYKNEKV EVAKPKVKQD LIDSKLAFVY NEPESLVISR
SGDECIVSLE DQWYTDYGEP NWKQRSLECL HQMNCYTPEV THAFENVFDW LKSWALSRTY
GLGSKIPWDP KYLVESLSDS TVYHAFYTIN HLLHTDYYGK VIGPLGITSD QMTDEVFDYI
FCRREDIKSD IPIEKLNILR REFEYFYPLD ISISAKDLIP NHLTFFIYCH TALFQKKFYP
KAIRSNGHLL LNNAKMSKST GNFMTLEQIV EKFGADASRI ALADAGDSIE DANFDESNAN
AAILRLTTLK DWSEQIVANI GDLRTGEFNY FDIAFENEMN QLIEETYKQY SLTNYKSALK
HGLFDYQVSR DYYREAVIVE GMHKDLALRY IETQSLMLQP IAPHFAEYIY REVLGNKEKS
AQFVAFPKSS KPVSKSIADS LEYVRSLSRS IRETEGAILK KKKGSKTAEV DSKSPVKLTI
LISSTFPDWQ DQYIELVRES FENHTLNDNS QLKQKINGKD MKRAMPFISL LKQRLTTEPA
EVVFNRKLSF DEIETVKKVL AILKRSTSIF NCQEIQAISF ASGETVGTDI ITGEKVEIVA
SPKVIEAAIP GQPGVVITN
//
