ID A0A1E4UYK8_9PSED Unreviewed; 368 AA.
AC A0A1E4UYK8;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Multifunctional fusion protein {ECO:0000256|RuleBase:RU363066, ECO:0000256|RuleBase:RU369079};
DE Includes:
DE RecName: Full=Gluconokinase {ECO:0000256|RuleBase:RU363066};
DE EC=2.7.1.12 {ECO:0000256|RuleBase:RU363066};
DE Includes:
DE RecName: Full=TRAP transporter small permease protein {ECO:0000256|RuleBase:RU369079};
GN ORFNames=A7D25_11765 {ECO:0000313|EMBL:OEC34782.1};
OS Pseudomonas sp. 21C1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1843690 {ECO:0000313|EMBL:OEC34782.1, ECO:0000313|Proteomes:UP000094259};
RN [1] {ECO:0000313|EMBL:OEC34782.1, ECO:0000313|Proteomes:UP000094259}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=21C1 {ECO:0000313|EMBL:OEC34782.1,
RC ECO:0000313|Proteomes:UP000094259};
RA Souza V., Ponce-Soto G.Y.;
RT "Genomic analysis from strains isolated from an enrichment experiment in
RT Cuatro Cienegas.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the tripartite ATP-independent periplasmic (TRAP)
CC transport system. {ECO:0000256|RuleBase:RU369079}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-gluconate = 6-phospho-D-gluconate + ADP + H(+);
CC Xref=Rhea:RHEA:19433, ChEBI:CHEBI:15378, ChEBI:CHEBI:18391,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58759, ChEBI:CHEBI:456216;
CC EC=2.7.1.12; Evidence={ECO:0000256|ARBA:ARBA00001329,
CC ECO:0000256|RuleBase:RU363066};
CC -!- PATHWAY: Carbohydrate acid metabolism; D-gluconate degradation.
CC {ECO:0000256|ARBA:ARBA00004875}.
CC -!- SUBUNIT: The complex comprises the extracytoplasmic solute receptor
CC protein and the two transmembrane proteins.
CC {ECO:0000256|RuleBase:RU369079}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|RuleBase:RU369079}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU369079}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the TRAP transporter small permease family.
CC {ECO:0000256|RuleBase:RU369079}.
CC -!- SIMILARITY: Belongs to the gluconokinase GntK/GntV family.
CC {ECO:0000256|ARBA:ARBA00008420, ECO:0000256|RuleBase:RU363066}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEC34782.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LXJR01000012; OEC34782.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1E4UYK8; -.
DR Proteomes; UP000094259; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046316; F:gluconokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd02021; GntK; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR006001; Therm_gnt_kin.
DR InterPro; IPR007387; TRAP_DctQ.
DR NCBIfam; TIGR01313; therm_gnt_kin; 1.
DR PANTHER; PTHR43442; GLUCONOKINASE-RELATED; 1.
DR PANTHER; PTHR43442:SF3; GLUCONOKINASE-RELATED; 1.
DR Pfam; PF13671; AAA_33; 1.
DR Pfam; PF04290; DctQ; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363066};
KW Cell inner membrane {ECO:0000256|RuleBase:RU369079};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU363066};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU369079};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363066};
KW Reference proteome {ECO:0000313|Proteomes:UP000094259};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363066};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU369079};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU369079}; Transport {ECO:0000256|RuleBase:RU369079}.
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369079"
FT TRANSMEM 250..268
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369079"
FT TRANSMEM 289..311
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369079"
FT TRANSMEM 323..348
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369079"
SQ SEQUENCE 368 AA; 39844 MW; 7D260041CC7309F4 CRC64;
MQHITAPAIP ADRRTLVIMG VSGSGKTEIS QGVAHTLGWR HIEADQFHPA ENVERMRAGI
PLSDDDRIHW LDALIEAMQA SQTAGESFVL ACSALKQRYR DRLRAAVPGL QFAHLDIDYA
TALQRVGGRA GHFMPTSLVD SQFATLESPA DEAHVLVVDA VQPREQVIAQ VCAWARRADT
TEQPDMAEID SANLAEPHAD PIYAGRVASA FDRLTDGLMA VLMGFMVVAV FGNVVLRYAF
GTGWAGAEEL SRLAFVWLVF VGVASSMRRG ELMRFSMLRD RFPLAARRLV DSASWLLVIA
ASLLAAMGAW SQVGYGWGNV STVVGYPVVL GMLPVLGSMV ALAVLALVQL ANVWRRGAPI
STPLNSLD
//
