ID A0A1E4V5W0_9PSED Unreviewed; 1171 AA.
AC A0A1E4V5W0;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=A7D25_01355 {ECO:0000313|EMBL:OEC37339.1};
OS Pseudomonas sp. 21C1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1843690 {ECO:0000313|EMBL:OEC37339.1, ECO:0000313|Proteomes:UP000094259};
RN [1] {ECO:0000313|EMBL:OEC37339.1, ECO:0000313|Proteomes:UP000094259}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=21C1 {ECO:0000313|EMBL:OEC37339.1,
RC ECO:0000313|Proteomes:UP000094259};
RA Souza V., Ponce-Soto G.Y.;
RT "Genomic analysis from strains isolated from an enrichment experiment in
RT Cuatro Cienegas.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEC37339.1}.
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DR EMBL; LXJR01000001; OEC37339.1; -; Genomic_DNA.
DR RefSeq; WP_069516081.1; NZ_LXJR01000001.1.
DR AlphaFoldDB; A0A1E4V5W0; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000094259; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR048472; DNA_pol_IIIA_C.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF20914; DNA_pol_IIIA_C; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF160975; AF1531-like; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000094259};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..73
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1171 AA; 130548 MW; E5912ADFC9DC72D8 CRC64;
MTASFVHLRL HTEFSLVDGL VRVKPLVKTV AGAGMPAVAV TDQSNMCSLV KFYKASMGAG
IKPICGADIW MASADEDGAL SRMTLLAMNP KGYRNLTELV SRGWTDGQSN DLVIIQREWV
KEAAEGLIAL SGAKEGEVGH ALLNGDRGEA EALLREWLAV FPERFYLEVQ RTSRVNDEEH
LHLAVELADR VGAPLVATND VRFLKQEDFA AHETRVCIGE GRSLDDPRRP RTYSDQQYLK
TPEEMAELFS DLPEALENTL EIAKRCNIDV QLGKYFLPDF PVPAGMTMDE YFRQVSFEGL
DERLAVLLPK DTPDYDAKKQ VYVDRLNFEL DIIIQMGFPG YFLIVMDFIK WAKNNGVPVG
PGRGSGAGSL VAYVQKITDL DPLAYDLLFE RFLNPERVSM PDFDVDFCMD GRDRVIDYVA
EKYGRNAVSQ IITFGTMAAK AVVRDVARAQ GKSYGLADRL SKMIPFEVGM TLEKAYEMEE
ALRDFLKVDE DAREIWEMSL KLEGICRGTG KHAGGVVIAP TKLTDFSPIA CDEEGGGLVT
QFDKDDVESA GLVKFDFLGL RTLTIIKWAM ETINREQAKQ GLPDLNIDFI PLDDKPTFAM
LQKAETTAVF QLESRGMKEL IKKLKPDCLE DMIALVALFR PGPLQSGMVD DFINRKHGRE
PISYPHQDYQ YAGLEPVLKP TYGIILYQEQ VMQIAQVMAG YTLGGADMLR RAMGKKKPEE
MAKQRGGFIE GCANNGIDAN LAGNIFDLVE KFAGYGFNKS HSAAYGLLSY QTAWLKAHHS
APFMAAVLSA DMHNTDKVVT LIEECRSMKL RIDAPDVNTS EFKFTVNEEG WIVYGLGAIK
GVGEGPVEAI VESRRDGPFK DLFDFCSRVD LKRINKRTLE ALIRSGALDR LGPYFHEELK
AYKANIDNNR AVLLAAMEEA VQAAEQTARS HDSGHMDLFG GVFAETTDVY GNHRRARELS
LKERLKGEKD TLGLYLTGHP IDEYESEVRR FARQRIVDLK PARGDQTIAG LIVNLRVMKN
KKGDKMGFIT LDDRSGRIEA SLFAEAFASN QALLQSDALV VVEGEVSNDD FSGGLRLRAK
RVMSLEEART GLADSLRLKI NNAALKGDRL RWLADVCSKH KGACPITLDY SSDDAKALLQ
FGEQWRIDPA DSLIQALRDQ FGRDNVFLHY R
//
