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Database: UniProt
Entry: A0A1E5AJ96_9RHOB
LinkDB: A0A1E5AJ96_9RHOB
Original site: A0A1E5AJ96_9RHOB 
ID   A0A1E5AJ96_9RHOB        Unreviewed;       268 AA.
AC   A0A1E5AJ96;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   24-JAN-2024, entry version 21.
DE   RecName: Full=Small-conductance mechanosensitive channel {ECO:0000256|RuleBase:RU369025};
GN   ORFNames=AB838_02360 {ECO:0000313|EMBL:OED50367.1};
OS   Rhodobacteraceae bacterium (ex Bugula neritina AB1).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC   Paracoccaceae.
OX   NCBI_TaxID=1868286 {ECO:0000313|EMBL:OED50367.1, ECO:0000313|Proteomes:UP000094333};
RN   [1] {ECO:0000313|EMBL:OED50367.1, ECO:0000313|Proteomes:UP000094333}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AB1-7 {ECO:0000313|EMBL:OED50367.1};
RX   PubMed=27681823; DOI=10.1038/srep34362;
RA   Miller I.J., Weyna T.R., Vanee N., Fong S., Lim-Fong G.E.;
RT   "Single sample resolution of rare microbial dark matter in a marine
RT   invertebrate metagenome.";
RL   Sci. Rep. 6:34362-34362(2016).
CC   -!- FUNCTION: Mechanosensitive channel that participates in the regulation
CC       of osmotic pressure changes within the cell, opening in response to
CC       stretch forces in the membrane lipid bilayer, without the need for
CC       other proteins. Contributes to normal resistance to hypoosmotic shock.
CC       Forms an ion channel of 1.0 nanosiemens conductance with a slight
CC       preference for anions. {ECO:0000256|RuleBase:RU369025}.
CC   -!- SUBUNIT: Homoheptamer. {ECO:0000256|RuleBase:RU369025}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000256|RuleBase:RU369025}; Multi-pass membrane protein
CC       {ECO:0000256|RuleBase:RU369025}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family.
CC       {ECO:0000256|ARBA:ARBA00008017, ECO:0000256|RuleBase:RU369025}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|RuleBase:RU369025}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OED50367.1}.
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DR   EMBL; MDLF01000032; OED50367.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1E5AJ96; -.
DR   STRING; 1868286.AB838_02360; -.
DR   Proteomes; UP000094333; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008381; F:mechanosensitive monoatomic ion channel activity; IEA:InterPro.
DR   Gene3D; 1.10.287.1260; -; 1.
DR   Gene3D; 2.30.30.60; -; 1.
DR   Gene3D; 3.30.70.100; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR049142; MS_channel_1st.
DR   InterPro; IPR049278; MS_channel_C.
DR   InterPro; IPR045275; MscS_archaea/bacteria_type.
DR   InterPro; IPR023408; MscS_beta-dom_sf.
DR   InterPro; IPR006685; MscS_channel_2nd.
DR   InterPro; IPR011066; MscS_channel_C_sf.
DR   InterPro; IPR011014; MscS_channel_TM-2.
DR   PANTHER; PTHR30221; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR   PANTHER; PTHR30221:SF1; SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL; 1.
DR   Pfam; PF21088; MS_channel_1st; 1.
DR   Pfam; PF00924; MS_channel_2nd; 1.
DR   Pfam; PF21082; MS_channel_3rd; 1.
DR   SUPFAM; SSF82689; Mechanosensitive channel protein MscS (YggB), C-terminal domain; 1.
DR   SUPFAM; SSF82861; Mechanosensitive channel protein MscS (YggB), transmembrane region; 1.
DR   SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane {ECO:0000256|RuleBase:RU369025};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Ion channel {ECO:0000256|RuleBase:RU369025};
KW   Ion transport {ECO:0000256|RuleBase:RU369025};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU369025};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW   ECO:0000256|RuleBase:RU369025};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|RuleBase:RU369025}; Transport {ECO:0000256|RuleBase:RU369025}.
FT   TRANSMEM        21..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU369025"
FT   TRANSMEM        60..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU369025"
FT   TRANSMEM        85..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU369025"
FT   DOMAIN          61..100
FT                   /note="Mechanosensitive ion channel transmembrane helices
FT                   2/3"
FT                   /evidence="ECO:0000259|Pfam:PF21088"
FT   DOMAIN          102..168
FT                   /note="Mechanosensitive ion channel MscS"
FT                   /evidence="ECO:0000259|Pfam:PF00924"
FT   DOMAIN          174..256
FT                   /note="Mechanosensitive ion channel MscS C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF21082"
SQ   SEQUENCE   268 AA;  28725 MW;  C7BDB4E28A6EF2BE CRC64;
     MEQMIEQAGA YWPLMVSGVK ALAVLILGWM ATGWISGVAR RRINNTPNID PTLGNFAASV
     IRWVLLAVVL VAVLGIFGIQ ATSLVAMLGA ATLAIGLALQ GTLSDLAAGF MLILFRPYKI
     GQYVDIGGTA GTVKDLNLFI TELATPDNVQ IIVPNGQAWG AIITNFSHHD TRRVDMVFGI
     DYGDSADAAK AIILEQAAAD ARVLKDPEPW VRVTNLGDSS VDLTARVWCD AADYWEVKFA
     LTQGVKEAFD AKGISIPYPH SVEIKKAG
//
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