ID A0A1E5D6T0_9VIBR Unreviewed; 201 AA.
AC A0A1E5D6T0;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Glutathione-regulated potassium-efflux system ancillary protein KefG {ECO:0000256|HAMAP-Rule:MF_01415};
DE AltName: Full=Putative quinone oxidoreductase KefG {ECO:0000256|HAMAP-Rule:MF_01415};
DE EC=1.6.5.2 {ECO:0000256|HAMAP-Rule:MF_01415};
GN Name=kefG {ECO:0000256|HAMAP-Rule:MF_01415};
GN ORFNames=A130_11425 {ECO:0000313|EMBL:OEE79337.1};
OS Vibrio genomosp. F6 str. FF-238.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=1191298 {ECO:0000313|EMBL:OEE79337.1, ECO:0000313|Proteomes:UP000094165};
RN [1] {ECO:0000313|EMBL:OEE79337.1, ECO:0000313|Proteomes:UP000094165}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FF-238 {ECO:0000313|EMBL:OEE79337.1,
RC ECO:0000313|Proteomes:UP000094165};
RX PubMed=22955834; DOI=10.1126/science.1219385;
RA Cordero O.X., Wildschutte H., Kirkup B., Proehl S., Ngo L., Hussain F.,
RA Le Roux F., Mincer T., Polz M.F.;
RT "Ecological populations of bacteria act as socially cohesive units of
RT antibiotic production and resistance.";
RL Science 337:1228-1231(2012).
CC -!- FUNCTION: Regulatory subunit of a potassium efflux system that confers
CC protection against electrophiles. Required for full activity of KefB.
CC {ECO:0000256|HAMAP-Rule:MF_01415}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + H(+) + NADH = a quinol + NAD(+);
CC Xref=Rhea:RHEA:46160, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01415};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + H(+) + NADPH = a quinol + NADP(+);
CC Xref=Rhea:RHEA:46164, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:132124; EC=1.6.5.2;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01415};
CC -!- SUBUNIT: Interacts with KefB. {ECO:0000256|HAMAP-Rule:MF_01415}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_01415}; Peripheral membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_01415}; Cytoplasmic side {ECO:0000256|HAMAP-Rule:MF_01415}.
CC -!- SIMILARITY: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefG
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01415}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEE79337.1}.
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DR EMBL; AJYW02000026; OEE79337.1; -; Genomic_DNA.
DR RefSeq; WP_017053162.1; NZ_AJYW02000026.1.
DR AlphaFoldDB; A0A1E5D6T0; -.
DR Proteomes; UP000094165; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0050136; F:NADH dehydrogenase (quinone) activity; IEA:RHEA.
DR GO; GO:0008753; F:NADPH dehydrogenase (quinone) activity; IEA:RHEA.
DR GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; IEA:UniProtKB-UniRule.
DR GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR Gene3D; 3.40.50.360; -; 1.
DR HAMAP; MF_01415; K_H_efflux_KefG; 1.
DR InterPro; IPR003680; Flavodoxin_fold.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR023947; K_H_efflux_KefG.
DR InterPro; IPR046980; KefG/KefF.
DR PANTHER; PTHR47307; GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM ANCILLARY PROTEIN KEFG; 1.
DR PANTHER; PTHR47307:SF1; GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM ANCILLARY PROTEIN KEFG; 1.
DR Pfam; PF02525; Flavodoxin_2; 1.
DR SUPFAM; SSF52218; Flavoproteins; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01415};
KW Cell membrane {ECO:0000256|HAMAP-Rule:MF_01415};
KW Membrane {ECO:0000256|HAMAP-Rule:MF_01415};
KW NAD {ECO:0000256|HAMAP-Rule:MF_01415};
KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01415};
KW Reference proteome {ECO:0000313|Proteomes:UP000094165}.
FT DOMAIN 13..179
FT /note="Flavodoxin-like fold"
FT /evidence="ECO:0000259|Pfam:PF02525"
SQ SEQUENCE 201 AA; 23138 MW; 56AF22E0129D84B3 CRC64;
MSDTQPQVAS PLKILVIYAH PEPHSSIANQ AMIKAIEPLG HVRVHDLYAT YPDFFIDVNF
EHQLLLEHDV IVFQHPLYMY SCPALLKEWF DRVLSKGFAF GHECALEGKY WRSVITTGGQ
EGAFGAQGYN KYPLEEILQP FELTASLCRM HWLDPLVLHW ARNINDKERE HHADRYRHWL
QDPLGKLSEQ LSTTDGDADG Q
//
