ID A0A1E5KWK1_9ENTE Unreviewed; 302 AA.
AC A0A1E5KWK1;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN ORFNames=BCR26_13800 {ECO:0000313|EMBL:OEH82231.1};
OS Enterococcus rivorum.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=762845 {ECO:0000313|EMBL:OEH82231.1, ECO:0000313|Proteomes:UP000095256};
RN [1] {ECO:0000313|EMBL:OEH82231.1, ECO:0000313|Proteomes:UP000095256}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 25899 {ECO:0000313|EMBL:OEH82231.1,
RC ECO:0000313|Proteomes:UP000095256};
RA Capua I., De Benedictis P., Joannis T., Lombin L.H., Cattoli G.;
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEH82231.1}.
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DR EMBL; MIEK01000025; OEH82231.1; -; Genomic_DNA.
DR RefSeq; WP_069698792.1; NZ_MIEK01000025.1.
DR AlphaFoldDB; A0A1E5KWK1; -.
DR STRING; 762845.BCR26_13800; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000095256; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028};
KW Reference proteome {ECO:0000313|Proteomes:UP000095256};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 13..77
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT ACT_SITE 136
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 302 AA; 34088 MW; 7F1144DE71B5D6F8 CRC64;
MEQINVKIKN ETGRIDKVLS ERLKGHSRSQ IQQWLKEENV RVNNELVKSN YKVKQEDEIV
INVPEPEVLD VLPENIPLTI VYEDQDVVVV NKPQGMVVHP SAGHPTGTLV NALLYHIKDL
SSINGVIRPG IVHRIDKDTS GLLMVAKNDK AHEALAEQLK DKTSLRKYVA LVHGEIGHDK
GEINAPIGRS KTDRKMQAVI EGGKPAVTHF EVLERFSDYT LVQLQLETGR THQIRVHMKY
IGYPVAGDPV YGPRRTLKGN GQFLHAEMLG FKHPTTGEMM VFEAPLPEIF EKTIEQLRNI
EE
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