ID A0A1E5R690_9ASCO Unreviewed; 478 AA.
AC A0A1E5R690;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=Chitobiosyldiphosphodolichol beta-mannosyltransferase {ECO:0000256|ARBA:ARBA00015841};
DE EC=2.4.1.142 {ECO:0000256|ARBA:ARBA00012611};
DE AltName: Full=Asparagine-linked glycosylation protein 1 {ECO:0000256|ARBA:ARBA00030745};
DE AltName: Full=Beta-1,4-mannosyltransferase {ECO:0000256|ARBA:ARBA00031566};
DE AltName: Full=GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase {ECO:0000256|ARBA:ARBA00033088};
DE AltName: Full=GDP-mannose-dolichol diphosphochitobiose mannosyltransferase {ECO:0000256|ARBA:ARBA00031434};
GN ORFNames=AWRI3578_g3391 {ECO:0000313|EMBL:OEJ82412.1};
OS Hanseniaspora opuntiae.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycodaceae; Hanseniaspora.
OX NCBI_TaxID=211096 {ECO:0000313|EMBL:OEJ82412.1, ECO:0000313|Proteomes:UP000095605};
RN [1] {ECO:0000313|Proteomes:UP000095605}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AWRI3578 {ECO:0000313|Proteomes:UP000095605};
RX PubMed=27856586; DOI=10.1128/genomea.01287-16;
RA Sternes P.R., Lee D., Kutyna D.R., Borneman A.R.;
RT "Genome sequences of three species of Hanseniaspora isolated from
RT spontaneous wine fermentations.";
RL Genome Announc. 4:E01287-E01287(2016).
CC -!- FUNCTION: Participates in the formation of the lipid-linked precursor
CC oligosaccharide for N-glycosylation. Involved in assembling the
CC dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic
CC surface of the ER. {ECO:0000256|ARBA:ARBA00024899}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GDP-alpha-D-mannose + N,N'-diacetylchitobiosyl
CC diphosphodolichol = beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-
CC GlcNAc-diphosphodolichol + GDP + H(+); Xref=Rhea:RHEA:13865,
CC Rhea:RHEA-COMP:9520, Rhea:RHEA-COMP:11044, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57269, ChEBI:CHEBI:57527, ChEBI:CHEBI:58189,
CC ChEBI:CHEBI:58472; EC=2.4.1.142;
CC Evidence={ECO:0000256|ARBA:ARBA00001259};
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000256|ARBA:ARBA00004922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEJ82412.1}.
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DR EMBL; LPNL01000008; OEJ82412.1; -; Genomic_DNA.
DR OrthoDB; 1219598at2759; -.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000095605; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004578; F:chitobiosyldiphosphodolichol beta-mannosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR InterPro; IPR026051; ALG1-like.
DR PANTHER; PTHR13036; BETA1,4 MANNOSYLTRANSFERASE; 1.
DR PANTHER; PTHR13036:SF0; CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE; 1.
DR Pfam; PF13692; Glyco_trans_1_4; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 4: Predicted;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676,
KW ECO:0000313|EMBL:OEJ82412.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OEJ82412.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
SQ SEQUENCE 478 AA; 55408 MW; 7236B6A230CA85A2 CRC64;
MRTGDYVLIV SSIIVSFIPI FFYYVLPFIK DTKHMNAFKR KACVIVIGPL VRSPRMMNHI
KALLLSGYDV EVISQATPNE LNELNSKYST IIKSIHXDDD QEXLNNVPTI NFHLLESEKY
KKQSYIMKAL SQMFTLLKMI WSVRSCDYAL VQNPPSIPIL PILITSKLIF NWKVIIDWHN
FGYSIFLMKG NSSKIKLLGN LYFKLELYFG RLADHHLVVT KMMADVLIKT WRWESKYPKV
EGDGFKVNDK ITVCYDYPDV QFQPYVNKVQ DRLELINNIP DVADLKNVDI TEYTIGVTST
SFTPDEDLQL LLDSLKDLDS ILKXKGKKFM LIITGKGPLK DXFIKSFEEQ XFHNIQYRFY
YLKLEDYPKI LQLADFGISL HSSSSGWDLP MKVWDMIGCG LPCVAYSFPA LDKELVHANK
TGLLFKDKSE LVHCLEEMLT NXDLMRXLTR NSLXNLNQMD RWVESWNKSV RKYIQPGC
//
