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Database: UniProt
Entry: A0A1E5RAK7_9ASCO
LinkDB: A0A1E5RAK7_9ASCO
Original site: A0A1E5RAK7_9ASCO 
ID   A0A1E5RAK7_9ASCO        Unreviewed;      2194 AA.
AC   A0A1E5RAK7;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   31-JUL-2019, entry version 17.
DE   SubName: Full=Glutamate synthase [NADH] {ECO:0000313|EMBL:OEJ83938.1};
GN   ORFNames=AWRI3579_g2963 {ECO:0000313|EMBL:OEJ83938.1};
OS   Hanseniaspora osmophila.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycodaceae; Hanseniaspora.
OX   NCBI_TaxID=56408 {ECO:0000313|EMBL:OEJ83938.1, ECO:0000313|Proteomes:UP000095728};
RN   [1] {ECO:0000313|Proteomes:UP000095728}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AWRI3579 {ECO:0000313|Proteomes:UP000095728};
RX   PubMed=27856586; DOI=10.1128/genomeA.01287-16;
RA   Sternes P.R., Lee D., Kutyna D.R., Borneman A.R.;
RT   "Genome sequences of three species of Hanseniaspora isolated from
RT   spontaneous wine fermentations.";
RL   Genome Announc. 4:E01287-E01287(2016).
CC   -!- COFACTOR:
CC       Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137;
CC         Evidence={ECO:0000256|PIRSR:PIRSR000187-2};
CC       Note=Binds 1 [3Fe-4S] cluster. {ECO:0000256|PIRSR:PIRSR000187-2};
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:OEJ83938.1}.
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DR   EMBL; LPNM01000008; OEJ83938.1; -; Genomic_DNA.
DR   OrthoDB; 126283at2759; -.
DR   Proteomes; UP000095728; Unassembled WGS sequence.
DR   GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0016040; F:glutamate synthase (NADH) activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0006537; P:glutamate biosynthetic process; IEA:InterPro.
DR   CDD; cd00982; gltB_C; 1.
DR   CDD; cd02808; GltS_FMN; 1.
DR   Gene3D; 1.10.1060.10; -; 1.
DR   Gene3D; 2.160.20.60; -; 1.
DR   Gene3D; 3.20.20.70; -; 2.
DR   Gene3D; 3.50.50.60; -; 2.
DR   Gene3D; 3.60.20.10; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR028261; DPD_II.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR017932; GATase_2_dom.
DR   InterPro; IPR002489; Glu_synth_asu_C.
DR   InterPro; IPR036485; Glu_synth_asu_C_sf.
DR   InterPro; IPR006982; Glu_synth_centr_N.
DR   InterPro; IPR012220; Glu_synth_euk.
DR   InterPro; IPR002932; Glu_synthdom.
DR   InterPro; IPR006005; Glut_synth_ssu1.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   Pfam; PF14691; Fer4_20; 1.
DR   Pfam; PF00310; GATase_2; 1.
DR   Pfam; PF04898; Glu_syn_central; 1.
DR   Pfam; PF01645; Glu_synthase; 1.
DR   Pfam; PF01493; GXGXG; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   PIRSF; PIRSF000187; GOGAT; 1.
DR   SUPFAM; SSF46548; SSF46548; 1.
DR   SUPFAM; SSF56235; SSF56235; 1.
DR   SUPFAM; SSF69336; SSF69336; 1.
DR   TIGRFAMs; TIGR01317; GOGAT_sm_gam; 1.
DR   PROSITE; PS51278; GATASE_TYPE_2; 1.
PE   4: Predicted;
KW   3Fe-4S {ECO:0000256|PIRSR:PIRSR000187-2};
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000095728};
KW   Iron {ECO:0000256|PIRSR:PIRSR000187-2};
KW   Iron-sulfur {ECO:0000256|PIRSR:PIRSR000187-2};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR000187-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000095728}.
FT   DOMAIN       97    495       Glutamine amidotransferase type-2.
FT                                {ECO:0000259|PROSITE:PS51278}.
FT   REGION        1     33       Disordered. {ECO:0000256|SAM:MobiDB-
FT                                lite}.
FT   COILED     1668   1691       {ECO:0000256|SAM:Coils}.
FT   COMPBIAS     16     33       Polar. {ECO:0000256|SAM:MobiDB-lite}.
FT   ACT_SITE     97     97       For GATase activity. {ECO:0000256|PIRSR:
FT                                PIRSR000187-1}.
FT   METAL      1236   1236       Iron-sulfur (3Fe-4S). {ECO:0000256|PIRSR:
FT                                PIRSR000187-2}.
FT   METAL      1242   1242       Iron-sulfur (3Fe-4S). {ECO:0000256|PIRSR:
FT                                PIRSR000187-2}.
FT   METAL      1247   1247       Iron-sulfur (3Fe-4S). {ECO:0000256|PIRSR:
FT                                PIRSR000187-2}.
SQ   SEQUENCE   2194 AA;  242426 MW;  B6AFF4D55A19C7DE CRC64;
     MSPPNAADHM QNGEYDMGIN ITSNTNTTTN TNTRHQSNRV VKSDKYDPME AQDCYEGGKP
     MDLETQNFLK DNENKSWAST IPRPVGLYNP DFEVDSCGTG FVAHIKGHAS HKIISDARPL
     LCNMAHRGAT STDGNGDGAG ILVGMPHKFM VREFQLDCQV KVPQQYHYSV GNVFFKKNCD
     LSGSKEIFET VASKFNLKVL AWREVPRDSS ILGSAALSRE PHILQPLVVD ADLDSDLDST
     FDEVKFQTNL YLLRKQASKK IGLENWFYIC SLSNKTIVYK GQLTPSQVYN YYYDLTNAHF
     ESHMALVHSR FSTNTFPSWD RAQPLRLLAH NGEINTLRGN KNWMRSKEGV MHSDTFGEDL
     ENLFPIVEEG GSDSAALDNV LELLTLNGLL SLPEAIMLCV PEATSLEMDS NLKAWYDWAA
     CLMEPWDGPA ALNFTDGRYC GAMLDRNGLR PCRYYITSDD RVICGSEVGV IPVANDLVIK
     KGKLIPGDIL LVDTQVGELV DTEKLKKNFA QRKDFKSWLS KIIKMEDLLS KTEKLLPFEN
     FHDYLLKNAN EMGESLEKVQ KDPRLLAMGY TFEQISLILV PMALNSKEAL GSMGNDAPLA
     CLNQDPVLLY DYFRQLFAQV TNPPIDSTRE TNVMSLECSV GPQGNLLDMN SSQCDRLLLK
     SPILNWKEFE SLKNIERAYP SWSIWQIDIT FPKSKGLLGY TEIIEKITEE ASQAIEQGKK
     MIMLSDRNLS ADRVAVSSMV AVGAIHHHLV RNKQRSQVAL VLETAEAREI HHFCCLLGYG
     VDAIFPYLAM ETLVKMNHEN LIRNANNDDI TIDDEKLIEN YQKAIDTGIL KTMSKMGIST
     LPSYKGAQIF EALGVDNSVV DLCFAGTASR IKGVTFEYIA QDAFSLHERG YPTRTIVEPS
     INLXEAGEYH WRDGGFKHMN DPTAIASLQD SVRNKNEKAW DMYVKKEMEA IRGCTLRGLL
     DLDFKNSTAI PLDQVEPWTE IARRFCTGAM SYGSISLEAH TTLAVAMNRL GAKSNCGEGG
     EDPERSIVHP NGDTMRSAIK QVASGRFGVT SYYLSDAEEI QIKIAQGAKP AVGGHLPGHK
     VTEQIAKTRH STAYVGLISP PPHHDIYSIE DLKQLIYDLK CSNPRATISV KLVSEVGVGI
     VAAGVAKAAA GNVNILISGH DGGTGAATWS DIKSTGLPWE LGLAESHQTL VLNDLRGHTV
     VQTDGQLRTG FDIAVAILLG AESFTLATIP LIAMGCIMLR KCHLNTCAVG VATQDPELRA
     KFKGQPEHVI NFFYYLINDL RKIMAKLGYR TVDEMIGHSE KLVKRETVNT KTINIDLNPI
     LTPAHTIRPG VVTKFTKKLE DKLFARLDNK LIDEAEITLD KGLPVSIDAS IINTDRALGS
     TLSYRVSKRF GPNGLPQDTV VVNIKGSAGQ SFGAFLAPGI TFILDGDAND YVGKGLSGGR
     IIIRPPPGSK FKSDENVIAG NTCFYGATSG YAFLSGCAGE RFAVRNSGAT MVVERIIGNN
     AFEYMTGGRA VVLSQLNSVN AFSGCTGGII YCLTSDHDAF RSKINKDTIE LSSLTDPVEI
     AFVKNLIKEH YNYTKSELAE RILKNFNHYL TSFIKVLPTD YKKVLEKEAA AKKLEEAKAT
     AAYLNKLEGG PCFENDATNG EVHALQNGQK KRALAVASHK ASLKEPKVAD LEDSIQDADQ
     LEKNMEKLDK IKGFMKYKLR HEVYRPSKSR TSDWKELSGP ISKKDAKYQT ARCMDCGVPF
     CTSDTGCPIS NVIPKFNELV FKNQWKLALD KLLETNNFPE FTGRVCPAPC QGACTLGINA
     DAVGIKSIER VIIDNAFKEG WIVPKPPTFK TGRSVAVIGS GPAGLACADQ LNRAGHSVTV
     YERNDRCGGL LMYGIPNMKL DKSIVQRRVD LLNAEGIEFI TNCQIGKDVT MDELKERFDS
     VVYATGSTIP RDLRIPGREL KNIDFAMQLL ETNTKALLNN DLEKVREQIK GKHVIVIGGG
     DTGNDCLGTS VRHEAASVLN FELLPQPPNE RAKDNPWPQW PRIMRVDYGH AEVKEHYGRD
     PREYCILSKE FVGNDEGEVT AIKTVRVEWK KSQSGVWQMV EVPGSEEVYQ ADIVLLSMGF
     VGPEMIEDAS VTKSKRGTID TVHDASYSVD GGKLFACGDC RRGQSLIVWA IQEGRKCAKA
     IDSFLMGSTN LPGNGGIVKR DYKMLEELAA SIPA
//
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