ID A0A1E5RCK4_HANUV Unreviewed; 2445 AA.
AC A0A1E5RCK4;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Serine/threonine-protein kinase MEC1 {ECO:0000256|ARBA:ARBA00021345};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
DE AltName: Full=ATR homolog {ECO:0000256|ARBA:ARBA00033001};
DE AltName: Full=DNA-damage checkpoint kinase MEC1 {ECO:0000256|ARBA:ARBA00030459};
DE AltName: Full=Mitosis entry checkpoint protein 1 {ECO:0000256|ARBA:ARBA00029679};
GN ORFNames=AWRI3580_g3496 {ECO:0000313|EMBL:OEJ84624.1};
OS Hanseniaspora uvarum (Yeast) (Kloeckera apiculata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycodaceae; Hanseniaspora.
OX NCBI_TaxID=29833 {ECO:0000313|EMBL:OEJ84624.1, ECO:0000313|Proteomes:UP000095358};
RN [1] {ECO:0000313|Proteomes:UP000095358}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AWRI3580 {ECO:0000313|Proteomes:UP000095358};
RX PubMed=27856586; DOI=10.1128/genomea.01287-16;
RA Sternes P.R., Lee D., Kutyna D.R., Borneman A.R.;
RT "Genome sequences of three species of Hanseniaspora isolated from
RT spontaneous wine fermentations.";
RL Genome Announc. 4:E01287-E01287(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC {ECO:0000256|ARBA:ARBA00010769}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEJ84624.1}.
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DR EMBL; LPNN01000007; OEJ84624.1; -; Genomic_DNA.
DR STRING; 29833.A0A1E5RCK4; -.
DR VEuPathDB; FungiDB:AWRI3580_g3496; -.
DR OrthoDB; 8448at2759; -.
DR Proteomes; UP000095358; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00892; PIKKc_ATR; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR012993; UME.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF124; SERINE_THREONINE-PROTEIN KINASE MEC1; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF08064; UME; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SMART; SM00802; UME; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00023204};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:OEJ84624.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000095358};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1427..2003
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 2101..2408
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 2413..2445
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
SQ SEQUENCE 2445 AA; 284615 MW; 6AE178539AF02F14 CRC64;
MDNMDILISK YKKQWKDIKN NTRSINKGSN TKLHWNYLSI NVELDTINNK DRSNIKYLNT
FIEKLNAETI VQDLKLFDNL IELITYFLLI NPQLFSQTFT KSSDKNSYFQ YGTANKTECV
WYLIGNVIEA FIKSFSNASI TLTNDLYESF FFKIIDTLTN NWLQIAVDIY GSSLEFEINK
VFQDQLMDSY LKLTMFINED QDEELDINDF INLLIRECIF LTIIINQDNN TKKNIXVINQ
HLRVILYLFD GLTEYLHQMN DEYSILLFEY DESLDDHKAN CKKLWFILGY FVKLLEKYFM
LNNLTHVNVD NLQFTMDFFK SILELLTNCS LSENETENLS VNFQVVLTST LNNVFDHVVL
MKCELKAIIE NTLGQYIYSL SLTFVSPLKN LFLKMNSNFN RKFETPSTAT NTHFIESLVK
KAPNIDSSSQ LFSQWKDAQE FFLFKIKESN VNVSEIGEII TLVSSLNCEF THNGRCLICD
YSDNYYSYLF KCNAFKRQQN SYGSSYPFKI LREVLNNCDS KRLSSEIILQ ENLLILLLKT
LNHNSKIDFL NDLSLLNDLE TSKQGNAGNL ALLKKLWDLI TFSFESTLIH LKQIASQVLV
LLVSTDFSAE TKLTKFVLKY LAQNNPKFSI PIVMDTIAKI TLNADISSPL RNVLLLKHLD
YIQDSVPPIK SDLAWSNINY ISKITDEYET TYQLLLPILP TVTNADVLKL DNNIIVFKNL
AVLTGRPFGE FLSRFLKYMI PSEIITYYKK DLIKKIANEI GIEKEEIISN SKLKSKIVSY
VITNGNCFKS KKVLRVLRNI SPDFYHKDLS EFDYYSVVTE IFQLYSNPAS LTNYKLEMNS
DLNFSNQLTL IKSFYYASLI QFHVANETQT IEEVEKRIEK FDSIESFKFW PFSEQELFYN
FLENAIIGIL HSLITILCDN SVTTITYDKS KAIDGLTIII IVSKPNQLMS VLPQIVMGLR
TGLESQELRH KSLKAWFFVL VKLGIQPLEY IIDVPITFFQ KHWEFLSDMT KDLFMQFTNQ
IFKYDESKIP YWTLMNFFFD DKLKVLERYP QKFSQILKSK NLFFDKIEAI NNHFHQVIIK
NDSFVIERNL KLLEFQLIKE VDICTSKDEK VXNILSQIGL AVFDLCYKFQ STDFNINLLA
VNCLSLLANR TSTMLKIQMS NIAEKNKGRN RINQVYDFSE QSTRILFMKR LIKYVLVPAF
WIVKTPIYQS YISFVIQEFL KQSKLSKLDE SSHSYRSIEI NEDLNRNFTD LERNIIEPMR
SSEFMITSNF VNYTPLKYPI YKKTMSVNTW LNSLIPDLIR RLAKNPHCEA NPIFINYLQL
NKCHEESIFF ETIFPYVIAQ VIMDDDGKLL YDWKLEVNHI FSIDLKVLNK HQVKNLRSIY
DNIFNAIDYV QKLKADLSNN LALKNDDQKA KIENLNLYIS KILFGGSIAS KALDVSAYER
SVLYMELSYR QNKNKMTSLE WKNNLNTAFS NMQELDALDG VEQFFTTNNS LDDKMDMLSK
SSTNWELAKE CFDELNTGWG GKVSSSSLEI LSENKMYGDI VQQFTTFAYS DLRLINDQTA
ISYNEASKLS IIALKATMLE SKVEMSNELC RAVEQTFAHP NTTLLLNYSL NKVLTNPNNT
DALNNLDLAR AVIGQRFISE FKTMTSLKMK DIVVNLDNVG LLNIMKYHSI LGNEQISSDS
LLFKKHKEMA NLMNKQRSTY LLDFNTIFDT EVFAVTLFSK FGMAHDEDIQ AKLFSFVEQA
RKNHKPEIAI KYLMKWFKNI NPNDSKDTLK FDFEYAKILW EAGEKEYALN IIKDYSDKIT
GKKIQLDKTT DNLKMFSAII RKYTQWCDTL NRKSPTELID QHLTFIEMGG EDDNSYFELG
LYYDRYYQQR QKTIPNKTSN DENFDNELIT NAIKFLLQSL TMTKQNKIIK EALPKVVILW
LDTVEKLSII KEDHPVIKNI NKMIKKAVID WDPNFWYTVI LQLISRILVP NKEARDIIVK
ILTTVFIAYP DYIVWYIVVL HNNRDDYIKQ AGNKIIQTFL KKTNKSGKLL VKNSKLLIDD
LSVICLKKSP DNPKNLIEDF NLDHLTSALP TSMAVPIQQN FSMMLPEFGL VDQMLISINE
IYPEYSVFKS LKKPKKITIK GNNGKHYYII CKKEDVRQDD QYMQFAQTMK YMLNNDAEAS
KRNMSISTYY IMPLNETYGI IEMVPSVVTI RSILTNSYDN RNLQKHRVQY LRQWNKIKKS
DTALKQLFEQ SLNVFKPVLY EWFLSKFPDP AKWYNCKNNF SRSYAVMCMV GHILGLGDRH
FDNILISEID GSVLHVDFDC LFEKGLDLPV PELVPFRLTN NIVDCLGITN VEGVFRKACE
LSMNLIRENE IMLLNEFEMM MIGRMKSLEQ AQKYTTQSSD HSLIYVGREG LSFEQILDVI
RNKMRGIDPK DSVTVSVAAQ VETLLKSAQD QELLYKMYSG WMPIW
//
