ID   A0A1E5RUG9_HANUV        Unreviewed;      1016 AA.
AC   A0A1E5RUG9;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 29.
DE   RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE            EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE   AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN   ORFNames=AWRI3580_g1265 {ECO:0000313|EMBL:OEJ90514.1};
OS   Hanseniaspora uvarum (Yeast) (Kloeckera apiculata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycodaceae; Hanseniaspora.
OX   NCBI_TaxID=29833 {ECO:0000313|EMBL:OEJ90514.1, ECO:0000313|Proteomes:UP000095358};
RN   [1] {ECO:0000313|Proteomes:UP000095358}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AWRI3580 {ECO:0000313|Proteomes:UP000095358};
RX   PubMed=27856586; DOI=10.1128/genomea.01287-16;
RA   Sternes P.R., Lee D., Kutyna D.R., Borneman A.R.;
RT   "Genome sequences of three species of Hanseniaspora isolated from
RT   spontaneous wine fermentations.";
RL   Genome Announc. 4:E01287-E01287(2016).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OEJ90514.1}.
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DR   EMBL; LPNN01000003; OEJ90514.1; -; Genomic_DNA.
DR   STRING; 29833.A0A1E5RUG9; -.
DR   VEuPathDB; FungiDB:AWRI3580_g1265; -.
DR   OrthoDB; 656at2759; -.
DR   Proteomes; UP000095358; Unassembled WGS sequence.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR   Gene3D; 1.10.730.20; -; 1.
DR   Gene3D; 3.40.50.620; HUPs; 2.
DR   Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   NCBIfam; TIGR00392; ileS; 1.
DR   PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW   ECO:0000256|RuleBase:RU363035};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|RuleBase:RU363035};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW   ECO:0000256|RuleBase:RU363035};
KW   Reference proteome {ECO:0000313|Proteomes:UP000095358}.
FT   DOMAIN          64..700
FT                   /note="Aminoacyl-tRNA synthetase class Ia"
FT                   /evidence="ECO:0000259|Pfam:PF00133"
FT   DOMAIN          746..844
FT                   /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT                   anticodon-binding"
FT                   /evidence="ECO:0000259|Pfam:PF08264"
SQ   SEQUENCE   1016 AA;  117536 MW;  62376F6B65B07DCD CRC64;
     MLNKARLCFK QRLYSTANDS KHIYQSTINL PACSFQVKNG SKYKINNDLN EDLTYKSSSL
     FYKQQYDEMR SNNTIKRDDL FILHDGPPFA NGDIHLGHAL NKTVKDVINR SNILHNNKYV
     HYKLGWDTHG LPIEQKVIQN DKLLRKKNKT LSPIEKDPLR LRQKCKELAL EQIEIQKKSF
     KKLAIMTNYD HYYTTLDKKY ELNELSLWNE MIKKNLIYVR NRPVKWSVEY TTALADAEIE
     FNANHVSHAA YVKFPILNQD NLYALIWTTT PWTLLANQAI GINTDMTYCK IQSKNNNEQY
     LVEKELFLAG KIPNTEGFKI IKEYKGDDAS ELLKLDYMNP FVKNGTPLKF ISHPTANNIS
     GTGLVHIAPS HGEIDYEISY ENNITPSTPV TEKGTYDYDP INKPNEWNFN IDFGNVRYPK
     VLDKDTGSKI FSFLKEKNLL LFDHEYIHSY PYDWRSKKPV ITRLTTQLYV DIDKIKKNAK
     ELLSNDKVSF KPATAKNTLS NYISNRDSWC ISRQRAWGLP IPCLNDDKGN MIIDERSTQH
     IIDKINEYGL EKWLDFDDKD MSMWLPXSTS KDLKKSKFTL DVWFDSGSSW KDLQDDFGFL
     NEKDITADLY LEGGDQHRGW FQSSLLIKLM TTGNKTPYKK VVTHGFVLDK TKTKMSKSIG
     NVIKPIELIE GAANKNLYSL DVDGLRMLLA CSNYTTDVSI GSDTVQVTYE LMKKIRNSLK
     YMIGSIAIED SKDFTLLPIN KLSAYDXSIL LKLQSNMKTI EKNYMDYEFG MVSKTVMEFL
     NTTMNTDYIL STKDTLFFEN KASSLRRKQV TSTFVYIIDY LRYSLSPIMP LTFQEVWDTF
     YVKXGLDCEV SGRILKGKTP FTKPWLVEGI IDHANIDKEL ILRDKFNESF KHFIKQSNVK
     DKNGNALTKT VDVDVVVNYK NIENLNRELI EELITASNIT YQKVDTPVEE VEYIKNENDE
     YERTVKLIEL KPDQEYLGIH LSVADKESCN RCWKKTVAKD EHICERCSNA ITTGTA
//